Cloning and Analysis of Two New PR10 Members in Soybean

  • CHEN Hua-tao ,
  • CHEN Xin ,
  • GU He-ping ,
  • ZHANG Hong-mei ,
  • YUAN Xing-xing ,
  • CUI Xiao-yan
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  • Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received date: 2013-05-16

  Online published: 2014-10-14

Abstract

Two new soybean genes named Glyma09g04530 and Glyma15g15590 which encoding PR10 proteins were cloned from a SMV resistant cultivar Kefeng No. 1. Glyma09g04530 and Glyma15g15590 have a 477 bp ORF and coding 158 amino acids. Sequence alignment and evolutionary tree clustering analysis showed that Gly-ma09g04530 and Glyma15g15590 are new PR10 protein genes in soybean. The genes of Glyma09g04530 and Gly-ma15g15590 expressed in root, stem and leave by using Real- time PCR analysis in soybean. Expression level of Gly-ma09g04530 and Glyma15g15590 were strongly induced and highly expressed in leaves of Kefeng No. 1 after inocu-lation SMV. We speculate that Glyma09g04530 and Glyma15g15590 may be involved for getting resistance to SMV.Key words: Soybean; PR10 protien; Gene cloning; Expression analysis

Cite this article

CHEN Hua-tao , CHEN Xin , GU He-ping , ZHANG Hong-mei , YUAN Xing-xing , CUI Xiao-yan . Cloning and Analysis of Two New PR10 Members in Soybean[J]. Acta Agriculturae Boreali-Sinica, 2013 , 28(4) : 7 -12 . DOI: 10.3969/j.issn.1000-7091.2013.04.002

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