With Chinese chive as an experimental material, four treatment factors including S ( 4g/m2 ) and nitrogen intensity ( 0,12, 24, 36 g/m2 ) were designed to study nitrate reduction and assimilation of Chinese chive in the experiment. The results showed that as the concentration of nitrogen rises, the nitrate content and the activity of nitrate reductase increased,while the activity of glutamic-oxaloacetic-transaminase (GOT) and glutamate-pyruvatetransaminase(GPT), the content of free amino acids and soluble protein, the dry content rate in Chinese chive increased at first and then descended. Sulphur can reduce nitrate accumulation and improve the activity of NR,GOT and GPT in Chinese chive under different nitrogen concentration,especially for 24 g/m2 of nitrogen. The increase amplitudes of NR,GOT and GPT activity , the content of free amino acids and soluble protein were 21.23%, 20.15%,17.74%,22.79%,25.27% respectively. In conclusion,S and suitable concentration of nitrogen improved nitrate reduction and assimilation,which,meanwhile,brought transaminations into play vigorously,and promoted turning NO 3-into free amino acid and dissolvable protein
Li Xiaofeng
,
Li Linyan
,
Xie Xin
,
Xue Zhanjun
,
Wang Junling
,
Gao Zhikui
. Effects of Sulfur and Nitrogen on Decreasing Nitrate Accumulation in Chinese Chive[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(3)
: 175
-178
.
DOI: 10.3969/j.issn.1000-7091.2013.03.031
[1] 高秀瑞,陈凤敏,刁春英,等. 不同形态氮素替代硝态氮对蔬菜硝酸盐含量变化的影响[J]. 华北农学报,2008, 23( 6) : 208 - 211.
[2] 都韶婷,金崇伟,章永松. 蔬菜硝酸盐积累现状及其调控措施研究进展[J]. 中国农业科学,2010,43 ( 17) :3580 - 3589.
[3] 王丰. 蔬菜中硝酸盐累积机制及控制方法研究[J].安徽农业科学, 2009, 37( 27) : 13028 - 13030.
[4] 武鹏鸣,杜忠东,贾田青. 施氮肥对蔬菜硝酸盐、亚硝酸盐积累的影响[J]. 山西农业科学, 2006, 34( 3) : 63 - 65.
[5] 张金盛,任顺荣,赵振达. 蔬菜保护地土壤硝酸盐积累及盐分变化[J]. 天津农业科学, 1998,4( 4) : 36 - 39.
[6] 张文新,张成军,赵同科. 缓释氮肥减少菜田土壤硝酸盐淋溶研究[J]. 华北农学报, 2010, 25( 5) : 166 - 170.
[7] 张素瑛,贾英,王美丽. 蔬菜硝酸盐污染状况及防治途径[J]. 山西农业科学, 2005, 33( 4) : 53 - 55.
[8] 王德芳,潘洁,陆文龙. 蔬菜硝酸盐污染及防治措施[J]. 天津农业科学, 2001,7( 1) : 29 - 32.
[9] 江立庚,曹卫星,甘秀芹,等. 不同施氮水平对南方早稻氮素吸收利用及其产量和品质的影响[J]. 中国农业科学, 2004, 37( 4) : 490 - 496.
[10] 姚春霞,郭开秀,赵志辉,等. 减量施肥对三种蔬菜硝酸盐含量、营养品质和生理特性的影响[J]. 水土保持学报, 2010, 24( 4) : 153 - 156.
[11] 黄东风,李卫华,邱孝煊. 不同硝、铵态氮水平配施对小白菜生长及硝酸盐累积的影响[J]. 土壤通报,2010, 41( 2) : 394 - 398.
[12] Wirtz M,Droux M,Hell R. O-acetylserine( thiol) lyase:an enigmatic enzyme of plant cysteine biosynthesis revisited in Arabidopsis thaliana[J]. Journal of Experimental Botany, 2004, 55: 1785 - 1798.
[13] Gilbert S M,Clarkson D T,Cambridge M. SO4 2 - deprivation has an early effect on content of Ribulose-1,5-Bisphosphale Carbonxylase /Oxygenase and photosynthesis in young leaves of wheat[J]. Plant Physiol,1997,115:1231 - 1239.
[14] 温月香,胡全才. 土施硫磺对甘蓝叶球NO3- N 含量的影响[J]. 农业环境与发展, 1999, 16( 2) : 36 - 38.
[15] 吴曦,陈明昌,杨治平. 碱性土壤施硫磺对油菜生 长、土壤pH 和有效磷含量的影响[J]. 植物营养与肥料学报, 2007, 13( 4) : 671 - 677.
[16] 陈明昌,张强,程滨,等. 土施有机添加剂和硫磺对小白菜生长和养分吸收的影响[J]. 植物营养与肥料学报, 2005, 11( 6) : 793 - 799.
[17] 张英鹏,徐旭军,林咸永,等. 供氮水平对菠菜产量、硝酸盐和草酸累积的影响[J]. 植物营养与肥料学报, 2004, 10( 5) : 494 - 498.
[18] 王朝辉,田霄鸿,李生秀,等. 两种蔬菜对硝态氮的累积和还原[J]. 西北农业大学学报, 1998( 2) : 12 - 16.
[19] Harada H,Yoshimura Y,Sunaga Y, et al. Variations in nitrogen uptake and nitrate-nitrogen concentration among sorghum groups[J]. Soil Sci Plant Nutr,2000,46 ( 1) :97 - 104.
[20] Ma X-M,Li L,Zhao P, et al. Effectofwater controlon activitiesofnitrogen assimilation enzymes and grain quality in winterwheat[J]. Acta PhytoecolSin, 2005, 29( 1) : 48 -53.
[21] 王朝辉,李生秀,田霄鸿. 不同氮肥用量对蔬菜硝酸盐累积的影响[J]. 植物营养与肥料学报,1998,4( 1) : 22 - 28.