Study the relationships between the shoot or root acid phosphatase activity ( APA) and phosphorus utilization ratio under different phosphorus treatments， to provide a basis for screening P efficiency varieties in soybean．Under three different P supply levels， the soybean shoot and root acid phosphatase activities and P utilization ratio of 12 genotypes were determinated and contrasted． The root acid phosphatase activities of 159 genotypes were also detected under 2 μmol /L P concentration． The results showed that soybean root acid phosphatase activity was positively correlated with P utilization ratio significantly ( r ＞ r0． 01) under 2 μmol /L P concentration． Six phosphorus efficiency varieties were screened out from 171 experimental genotypes． Under low phosphorus concentration， soybean root acid phosphatase activity coule be used as an important biochemistry index to screen P efficient genotype．
Liu Yuan
,
Li Xihuan
,
Zhang Caiying
. Relativity Analysis between Acid Phosphatase Activity and Screening Genotypic Variation in P Efficiency of Soybean[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(3)
: 145
-151
.
DOI: 10.3969/j.issn.1000-7091.2013.03.026
[1] 黄宇，张海伟，徐芳森． 植物酸性磷酸酶的研究进展[J]． 华中农业大学学报， 2008， 27(1) : 148 - 154．
[2] 樊明寿，徐冰，王艳． 缺磷条件下玉米根系酸性磷酸酶活性的变化[J]． 中国农业科技导报， 2001，3(3) : 33 -36．
[3] Song J Y ， Shawn M K． Induction of maize acid phosphatase activities under phosphorus starvation[J]． Plant and Soil，2001， 237: 109 - 115．
[4] 魏志强，史衍玺，孔凡美． 缺磷胁迫对花生磷酸酶活性的影响[J]． 中国油料作物学报， 2002， 24(3) : 44 - 46．
[5] 孙海国，张福锁． 缺磷条件下的小麦根系酸性磷酸酶活性研究[J]． 应用生态学报， 2002， 13(3) : 379 - 381．
[6] 周建朝，韩晓日，奚红光． 磷营养水平对不同基因型甜菜根磷酸酶活性的效应． 植物营养与肥料学报， 2006，12(2) : 233 - 239．
[7] Helal H M． Varietal difference in root s phosphatase activity as related to t he utilization of organic phosphorus[M]/ /BASSAM N． Genetic aspect s of plant mineral nut rition． Net herlands: Kluwer Academic Publishers， 1990．
[8] 丁洪，李生秀，郭庆元，等． 酸性磷酸酶活性与大豆耐低磷能力的相关研究[J]． 植物营养与肥料学报，1997，3(2) : 123 - 127．
[9] Mclachlan K D． Acid phosphatase activity of intact root sand phosphorus nutrition in plants． 2 Variations among wheat roots[J]． Australian Journal of Agricultural Research，1980， 31( 3) : 441 448．
[10] Yan X L ，Liao H ，Trull M C ， et al． Induction of a major leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean[J]． Plant Physiology，2001， 125: 1901 1911．
[11] McLachlan K D，Elliott D E，Marco D G D， et al． Leaf acid phosphatase isozymes in the diagnosis of phosphorus status in field-grown wheat[J]． Aust J Agric Res，1987， 38: 1 - 13．
[12] Jungk A，Barber S A． Plant age and the phosphorus uptake characteristics of trimmed and untrimmed corn root systems[J]． Plant Soil， 1975， 42: 227 - 239．
[13] 赵世杰，刘华山，董新纯，等． 植物生理学实验指导[M]． 北京: 中国农业出版社， 1998: 69 - 71．
[14] Furlani A M C，Machado C T T． Variabilidade e heranca da eficiencia na absorcao e utilizacao de nutrientes em ambientes marginais，em germoplasma de soja， trigo，arrozemilho[J]． Topicos em ciencia do solo，2002，2: 337- 391．
[15] Hocking P J，Randall P J． Better growth and phosphorus nutrition of sorghum and wheat following organic acid secreting crops[J]． Developments in Plant and Soil Sciences，2002， 92: 548 - 549．
[16] Xiu R W，Ying X W， Jiang T， et al． Over expressing At-PAP15 enhances phosphorus efficiency in soybean[J]．Plant Physiology， 2009， 151: 233 - 240．
[17] Huet T，Wei Q Q，Brendena H， et al． Biochemical and molecular characterization of AtPAP12 And AtPAP26:the predominant purple acid phosphatase Isozymes secreted by phosphate-starved Arabidopsis thaliana[J]．Plant Cell and Environment， 2010， 33: 1789 - 1803．
[18] Hui F ZH，Wei Q Q，Xu ZH L， et al． Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower[J]． Plant Molecular Biology， 2005， 59: 581 -594．
[19] Vance C P，Uhde-stone C，Allan D L． Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource[J]． New Phytologist， 2003， 157:423 -447．
[20] Giuseppe D，Claus K M，Preben B M， et al． Cloning and characterization of purple acid phosphatase phytases from wheat，barley，maize，and rice[J]． Plant Physiology，2011， 156: 1087 - 1100．
[21] Zhang H W，Huang Y，Ye X SH， et al． Analysis of the contribution of acid phosphatase to P efficiency in Brassica napus under low phosphorus conditions[J]． Science China， 2010， 53(6) : 709 - 717．
[22] Hadjiivanova C，Tzvetkova N． Acid phosphatase activity in sun-exposed and shade beech ( fagus sylvatical) leaves[J]． General and Applied Plant Physiology，2010，36( 1 - 2) : 24 - 27．
[23] Djary M． K，Jean T．G，Betty M． F，et al． Biochemical characterization of two non-specific acid phosphatases from Cucurbitaceae ( Lagenaria siceraria) edible seeds exhibiting phytasic activity[J]． Journal of Animal ＆ Plant Sciences， 2010，7(3) : 860 - 875．
[24] Kenjior T，Yuji M，Masashi U， et al． Occurrent of acidphosphatase inhibitors in soybeans[J]． Biosci Biotech Biochem， 1997， 61( 2) : 387 - 388．
[25] Mariusz O，Bronislawa M，Wieslaw W． Plant purple acid phosphatases-genes， structures and biological function[J]．Acta Biochimica Polonica， 2003， 50(4) : 1245 -1256
