Study the relationships between the shoot or root acid phosphatase activity ( APA) and phosphorus utilization ratio under different phosphorus treatments, to provide a basis for screening P efficiency varieties in soybean.Under three different P supply levels, the soybean shoot and root acid phosphatase activities and P utilization ratio of 12 genotypes were determinated and contrasted. The root acid phosphatase activities of 159 genotypes were also detected under 2 μmol /L P concentration. The results showed that soybean root acid phosphatase activity was positively correlated with P utilization ratio significantly ( r > r0. 01) under 2 μmol /L P concentration. Six phosphorus efficiency varieties were screened out from 171 experimental genotypes. Under low phosphorus concentration, soybean root acid phosphatase activity coule be used as an important biochemistry index to screen P efficient genotype.
Liu Yuan
,
Li Xihuan
,
Zhang Caiying
. Relativity Analysis between Acid Phosphatase Activity and Screening Genotypic Variation in P Efficiency of Soybean[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(3)
: 145
-151
.
DOI: 10.3969/j.issn.1000-7091.2013.03.026
[1] 黄宇,张海伟,徐芳森. 植物酸性磷酸酶的研究进展[J]. 华中农业大学学报, 2008, 27(1) : 148 - 154.
[2] 樊明寿,徐冰,王艳. 缺磷条件下玉米根系酸性磷酸酶活性的变化[J]. 中国农业科技导报, 2001,3(3) : 33 -36.
[3] Song J Y , Shawn M K. Induction of maize acid phosphatase activities under phosphorus starvation[J]. Plant and Soil,2001, 237: 109 - 115.
[4] 魏志强,史衍玺,孔凡美. 缺磷胁迫对花生磷酸酶活性的影响[J]. 中国油料作物学报, 2002, 24(3) : 44 - 46.
[5] 孙海国,张福锁. 缺磷条件下的小麦根系酸性磷酸酶活性研究[J]. 应用生态学报, 2002, 13(3) : 379 - 381.
[6] 周建朝,韩晓日,奚红光. 磷营养水平对不同基因型甜菜根磷酸酶活性的效应. 植物营养与肥料学报, 2006,12(2) : 233 - 239.
[7] Helal H M. Varietal difference in root s phosphatase activity as related to t he utilization of organic phosphorus[M]/ /BASSAM N. Genetic aspect s of plant mineral nut rition. Net herlands: Kluwer Academic Publishers, 1990.
[8] 丁洪,李生秀,郭庆元,等. 酸性磷酸酶活性与大豆耐低磷能力的相关研究[J]. 植物营养与肥料学报,1997,3(2) : 123 - 127.
[9] Mclachlan K D. Acid phosphatase activity of intact root sand phosphorus nutrition in plants. 2 Variations among wheat roots[J]. Australian Journal of Agricultural Research,1980, 31( 3) : 441 448.
[10] Yan X L ,Liao H ,Trull M C , et al. Induction of a major leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean[J]. Plant Physiology,2001, 125: 1901 1911.
[11] McLachlan K D,Elliott D E,Marco D G D, et al. Leaf acid phosphatase isozymes in the diagnosis of phosphorus status in field-grown wheat[J]. Aust J Agric Res,1987, 38: 1 - 13.
[12] Jungk A,Barber S A. Plant age and the phosphorus uptake characteristics of trimmed and untrimmed corn root systems[J]. Plant Soil, 1975, 42: 227 - 239.
[13] 赵世杰,刘华山,董新纯,等. 植物生理学实验指导[M]. 北京: 中国农业出版社, 1998: 69 - 71.
[14] Furlani A M C,Machado C T T. Variabilidade e heranca da eficiencia na absorcao e utilizacao de nutrientes em ambientes marginais,em germoplasma de soja, trigo,arrozemilho[J]. Topicos em ciencia do solo,2002,2: 337- 391.
[15] Hocking P J,Randall P J. Better growth and phosphorus nutrition of sorghum and wheat following organic acid secreting crops[J]. Developments in Plant and Soil Sciences,2002, 92: 548 - 549.
[16] Xiu R W,Ying X W, Jiang T, et al. Over expressing At-PAP15 enhances phosphorus efficiency in soybean[J].Plant Physiology, 2009, 151: 233 - 240.
[17] Huet T,Wei Q Q,Brendena H, et al. Biochemical and molecular characterization of AtPAP12 And AtPAP26:the predominant purple acid phosphatase Isozymes secreted by phosphate-starved Arabidopsis thaliana[J].Plant Cell and Environment, 2010, 33: 1789 - 1803.
[18] Hui F ZH,Wei Q Q,Xu ZH L, et al. Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower[J]. Plant Molecular Biology, 2005, 59: 581 -594.
[19] Vance C P,Uhde-stone C,Allan D L. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource[J]. New Phytologist, 2003, 157:423 -447.
[20] Giuseppe D,Claus K M,Preben B M, et al. Cloning and characterization of purple acid phosphatase phytases from wheat,barley,maize,and rice[J]. Plant Physiology,2011, 156: 1087 - 1100.
[21] Zhang H W,Huang Y,Ye X SH, et al. Analysis of the contribution of acid phosphatase to P efficiency in Brassica napus under low phosphorus conditions[J]. Science China, 2010, 53(6) : 709 - 717.
[22] Hadjiivanova C,Tzvetkova N. Acid phosphatase activity in sun-exposed and shade beech ( fagus sylvatical) leaves[J]. General and Applied Plant Physiology,2010,36( 1 - 2) : 24 - 27.
[23] Djary M. K,Jean T.G,Betty M. F,et al. Biochemical characterization of two non-specific acid phosphatases from Cucurbitaceae ( Lagenaria siceraria) edible seeds exhibiting phytasic activity[J]. Journal of Animal & Plant Sciences, 2010,7(3) : 860 - 875.
[24] Kenjior T,Yuji M,Masashi U, et al. Occurrent of acidphosphatase inhibitors in soybeans[J]. Biosci Biotech Biochem, 1997, 61( 2) : 387 - 388.
[25] Mariusz O,Bronislawa M,Wieslaw W. Plant purple acid phosphatases-genes, structures and biological function[J].Acta Biochimica Polonica, 2003, 50(4) : 1245 -1256