Construction of a Genetic Linkage Map in Cabbage Based on AFLP,SSR and SRAP Markers

  • Zhu Hongyun ,
  • Xie Jianming ,
  • Yu Jihua ,
  • Jian Yuancai ,
  • Kang Jungen
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  • 1.Gansu Agriculture University, Lanzhou 730070,China;
    2.Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture, Beijing 100097,China

Received date: 2013-03-21

  Revised date: 2013-03-21

  Online published: 2014-10-14

Abstract

A F2 population consisting of 142 individual plants derived from a cross between fusarium wilt resistant cabbage self line R4-P1 and fusarium wilt susceptible line R2-P2 combining with AFLP , SSR and SRAP markers were applied to construct a high density genetic linkage map of Brassica oleracea. In total, 240 markers including 162 AFLP markers, 52 SSR markers and 26 SRAP markers were integrated into 9 groups( LOD≥4) . It covered 864. 4 cM,with the average interval of 3. 6 cM. It is a relatively high dense map involving more kinds of markers especially more reference SSR markers. It is also one of the most comprehensive intrasific genetic linkage map of covering whole genome of cabbage. It will build a good platform for mapping and cloning of the fusarium wilt resistant gene in cabbage, and furthermore,lay the foundation for mapping important agronomic traits for marker assistant breeding.

Cite this article

Zhu Hongyun , Xie Jianming , Yu Jihua , Jian Yuancai , Kang Jungen . Construction of a Genetic Linkage Map in Cabbage Based on AFLP,SSR and SRAP Markers[J]. Acta Agriculturae Boreali-Sinica, 2013 , 28(3) : 82 -87 . DOI: 10.3969/j.issn.1000-7091.2013.03.016

References

[1] Slocum. Linkage arrangement of restriction fragement length polymorphism loci in Brassica oleracea[J]. Theor Appl Genet, 1990, 80: 57 - 64.
[2] Kianian S F,Quiros C F. Generation of a Brassica oleracea composite RFLP map: linkage arrangements among various populations and evolution implications[J]. Theor Appl Genet, 1992, 84: 544 - 544.
[3] Voorrips R E, Jongerius M C,Kanne H J. Mapping of two genes for resistance to clubroot( PlasmodioPhora brassieae) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers[J]. Theor Appl Genet, 1997, 94: 75 - 82.
[4] Hu J,Sadowski J,Osborn T C, et al. Linkage group alignment from four independent Brassica RFLP maps [J]. Genome,1998, 41: 22 - 235.
[5] 胡学军,邹国林. 甘蓝分子连锁图的构建与品质性状的QTL 定位[J]. 武汉植物学研究, 2004, 22( 6) : 482 -485.
[6] 缪体云. 结球甘蓝遗传图谱的构建及主要农艺性状的QTL 定位[D]. 北京: 中国农业科学院, 2007.
[7] 李梅. 结球甘蓝抽薹开花性状的遗传、QTL 定位及生理研究[D]. 北京: 中国农业科学院, 2009.
[8] Gao M,Li G,Yang B, et al,High-density Brassica oleracea linkage map: identification of useful new linkages[J]. Theor Appl Genet, 2007( 115) : 277 - 287.
[9] Wang X W,Lou P,Bonnema G, et al. Linkage mapping of a dominant male sterility gene Ms-cd1 in Brasscia oleracea[J]. Genome, 2005, 48: 848 - 854.
[10] Heusden A W van,Ooijen J W van,Vrielink-van Ginkel R, et al. A genetic map of an interspecific cross in Allium based OD amplified fragment 1ength polymorphism( AFLP) markers[J]. Theoretical and Applied Geneties,2000, 100: 118 - 126.
[11] Saal B,Plieske J,Hu J, et al,Microsatellite markers for genome analysis in Brassica, II. Assignment of rapeseed microsatellite to the A and C genomes and genetic mapping in Brassica oleracea L. [J]. Theor Appl Genet,2001, 102(5) : 695 - 699.
[12] LI G,Quiros C F. Sequence-related amplified polymorphism(SRAP) ,a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theor Appl Genet, 2001(103) : 455- 461.
[13] Bassam B J,Caetano-A nolles G,Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamide gels [J]. Analytical Biochemistry, 1991, 196( 1) : 80 -83.
[14] Landry B S,Hubert N,Crete R, et al. A geneticmap for Brassica oleracea basedon RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora Brassica ( Woronin)[J]. Genome, 1992, 35: 409 - 420.
[15] Camargo L E A,Savides L, Jung G, et al. Location of the selfincompatibility locus in an RFLP and RAPD map of Brassica oleracea[J]. J Hered, 1995, 88(1) : 57 - 59.
[16] Fang X J,Wu W R,Tang J L. Crop markers assisted breeding[M]. Beijing: Science Press, 2001.
[17] Foisset N,Delourme R,Barret P, et al. Molecular mapping analysis of Brassica napus using isozyme,RAPD and RFLP markers on double haploid progeny. Theoretical and Applied Genetics , 1996, 93: 1017 - 1025.
[18] Ajisaka H,Kuginuki Y,Shiratori M, et al. Mapping of loci affecting the cultural efficiency of microspore culture of Brassica rapa L. syn. campestris L. using DNA polymorphism[J]. Breed Sci, 1999, 49: 187 - 192.
[19] Xu S J,Singh R J,Hymowitz T. Establishment of a cytogenetic map soybean: progress and prospective[J]. Soybean Genetics Newsletter, 1997, 24: 121 - 122.
[20] Knox M R,Ellis T H N. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations[J]. Genetics, 2002, 162: 861 - 873.
[21] Lander E S,Green P,Abrahamson J, et al. MAPMAKER:an interactive computer package for constructing primary genetic linkage maps with experimenta1 and natural populations[J]. Genomics, 1987(1) : 174 - 181
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