The Gene Cloning and Bioinformatics Analysis of Endo-β-1,4 -glucanas Gene of Chili Pear

  • Zhang Xiaofei ,
  • Zhang Xianan ,
  • Wang Ran ,
  • Zhang Xinfu ,
  • Yang Shaolan
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  • College of Horticulture,Qingdao Agricultural University, Qingdao 266109,China

Received date: 2013-03-07

  Revised date: 2013-03-07

  Online published: 2014-10-14

Abstract

One full-length cDNA sequence of homologous gene endo-β-1,4-glucanas from Pyrus bretschneideri Rehd. Cv. Chili pear was cloned by polymerase chain reaction ( PCR) and RACE method,which was named as PbEG. The PbEG was 1 971 bp and the open reading frame encoded 493 amino acids,while the estimated molecular weight and isoelectric point of the putative protein were 54. 596 1 kDa and 9. 14. The protein encoded by PbEG belonged to Glycosyl-hydrolases-family-9. There was no membrane-spanning domain in the protein but a signal peptide in the N-terminus. And the deduced amino acids homology analysis indicated that PbEG has a high homology with RcEG(84%).

Cite this article

Zhang Xiaofei , Zhang Xianan , Wang Ran , Zhang Xinfu , Yang Shaolan . The Gene Cloning and Bioinformatics Analysis of Endo-β-1,4 -glucanas Gene of Chili Pear[J]. Acta Agriculturae Boreali-Sinica, 2013 , 28(3) : 53 -57 . DOI: 10.3969/j.issn.1000-7091.2013.03.010

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