In this study ,the tolerance to aluminum of eight accessions of 0. rufipogou indigenous to Gaozhou was identified by culturing in simple CaCI2solution,and some important agronomic traits of rice plants cultured in pot soils with low pH or adding A1 were investigated , Al-olerance related QTLs were also mapped primarily by using the advanced backcross population BC3F3 between Huajingxian 74 and accessions of 0. rufipogou indigenous to Gaozhou. The results were as follow. After seedling roots of 0. rufipogon were treated in 0.5 mmol/L CaCl2 solution with 50 μ mol/L A3+(pH=4.5) for 24 h , the relative root elongation(RRE) of eight accessions were all higher than 0.5.Meanwhile,RRE of the regenerative roots from tillerings of the four out of the eight accessions also higher than 0.5 .It is considered that the experimental accessions of 0. rufipgogon were A1 tolerant.Investigation of plant traits in the pot soil cultured experiments showed that the rice plant height was obviously affected by A1 stress ,also as seed setting rate and 1000 rain weight. Four QTLs related to Al-olerance were mapped in BC3+F3+ population by the method of MCIM. Hereinto,qRRE-6-2and qRRE-7-2,which were mapped in BC3+ F3+ population derived from Huajingxian 74 x GZW003,qRRE-4and qRRE-2-2,which were mapped in BC3F3 population derived from Huajingxian 74 x GZW006 and Huajingxian 74 x GZW087,respectively. These four QTLs explained 18. 33%,9. 18%,19. 02% and 24. 88% of the phenotypic variance,respectively. Except of qRRE-6-2,the favorable alleles of the other three QTLs were all derived from the donor parent 0.rufipogon.
Chu Shaowei
,
Wang Lin
,
Liu Guifu
,
Liu Xiangdong
,
Lu Yonggen
,
Fu Xuelin
. Aluminum Tolerance Identification and QTL Mapping in Oryza ru fipogon Indigenous to Gaozhou[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(3)
: 12
-18
.
DOI: 10.3969/j.issn.1000-7091.2013.03.003
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