The Peach some varieties were used to adopt uniform design,the SRAP2PCR reaction system Taq DNA polymerase,the template DNA,dNTPs,Mg2 +,primers five components to optimize the concentration. The results showed that 10μL Peach SRAP reaction system of the best components,including 215μL 10 ×PCR Buffer + Mg2 + U Taq DNA polymerase,40 ng template DNA,012 mmol/ L dNTPs,012μmol/ L primer1Established by the use of the system to other parts of peach germplasm to amplify the results of clear and reliable,good polymorphism1And using random selection of the SRAP primer and SSR primer respectively distinction between identification of eight kinds of peach germplasm and found that SRAP can be used to separate peach germplasm,and the SSR was used to separate,that the peach variety iden2 tification on the SRAP in more than SSR Convenient,and relatively simple operation,the applicability.
GUO Rui
,
LI Xiao-yan
,
WANG Li-rong
,
WANG Guo-shan
. Peach SRAP System Optimization and SSR Peach Varieties in the Comparative Identification[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 102
-105
.
DOI: 10.7668/hbnxb.2009.04.021
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