Dehydrin is a LEA protein in plant, it can substantial expression in the late embryo development period and stress environment in plants. First, the primers were designed according to the sequence of Ammopiptanthus mongolicus dehydrin cDNA library, the dehydrin gene was amplified with PCR, then cloned into E. coli Top10 by using pGM-T vector. Sequence analysis shows that the cloned 760 bp,which the same size as the known cDNA library Ammopiptanthus mongolicus dehydrin gene size. It note that this gene sequence without intron. the recombinant plasmid of cloned digested by Bgl ??and Best ??same as the pCAMBIA3301 plasmid, then linked by T4DNA ligase. Now, we successfully constructed the expression vector of dehydrin gene,To lay the foundation for the research of resistance to stress environment.
QIAO Hui-lei
,
WANG Rui-gang
,
GUO Jiu-feng
,
SUN Guo-qin
,
LI Guo-jing
. The Cloning and Expression Vector of Ammopiptanthus mongolicus Dehydrin Gene[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 88
-91
.
DOI: 10.7668/hbnxb.2009.04.018
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