:In order to further study expressed level and condition of Glucose oxidase (GOD) from Aspergillus niger 31758 in E. coli,the coding region of GOD from A. niger 3. 758 was inserted into the prokaryotic expression vector pET2 11a,and the recombinant plasmid was transformed into E. coli BL21 (DE3) plysS. The result of SDS2PAGE showed that the GOD gene was expressed by IPTGinduction,and the molecular weight of the fusion protein was about 66. 5 kDa.An2 tiserum was produced in rabbit immunized with commercial GOD,and ELISA analysis proved that the titer of this antibody was 106. The Western2blotting result confirmed that the antibody reacted specifically to the expressed fusion protein. The study established basis for preparation of GOD antibody from Aspergillus niger 3. 758.
AN Yu-lin
,
SUN Rui-fen
,
ZHANG He-ling
,
GUO Shu-chun
,
YAN Su-li
. Prokaryotic Expression and Western-blot Analysis of Glucose Oxidase Gene from Aspergillus niger[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 84
-87
.
DOI: 10.7668/hbnxb.2009.04.017
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