With untransformed rice and transgenic rices with different C4 photosynthesis genes as the materials, the activity of C4 photosynthesis enzymes, the net photosynthetic rate ( Pn) and the indexes of act ive oxygen metabolism were determined.The activities of C4 photosynthesis enzymes in untransformed rice were very low, while those of corresponding enzymes in different transgenic rices were all highly expressed. The Pn of the PPDK transgenic rice was not increased, that of the ME transgenic rice was decreased by 6. 1%, that of the transgenic ricewith PEPC and PPDK genes( CK) was simi?? lar with that of the PEPC transgenic rice under high light intensity. After treatment with ATP and its activator NaHSO3, the photosynthetic rate of O2 evolution were significant ly increased in the CK transgenic rice and the transgenic rice with PEPC, PPDK andME genes( CKM), which reached 80. 7% of that in maize and exhibited photosynthetic character of C4?? like plants.This indicated that ATP is the key limited factor for construct ing C4??like rice. In addition, after MV( artificial photooxidative activator) treatment, the CKM transgenic ricewas more tolerant to photooxidative stress.These results would provide a new technical approach to constructing C4??like rice.
ZHANG Bian-jiang
,
LING Li-li
,
CHEN Quan-zhan
,
HUA Chun
,
JIAO De-mao
. A Key Limited Factor ATP of Constructing C4-like Rice[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 17
-22
.
DOI: 10.7668/hbnxb.2009.04.004
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