The degenerate primers were designed based on the published information of sequence of Solanaceae plants. The cDNA clones encoding UDP2glucose : flavonoid 32O2glucosyltransferase ( 3GT) have been isolated from Solanum tuberosum cv. Chieftain with the degenerate primers by RT2PCR. The cDNA clone of 3GT encodes a predicted polypeptides of 448 amino acids,and the deduce protein showed 76 % highest identity with 3GT of Petunia hybrida and Solanum melongena,and the multiple alignment and phylogenetic analysis demonstrated that it belong to a new member of the corresponding 3GT2family enzymes. The mRNA expression analysis demonstrated that the accumulation of 3GT tran2 scripts in root,leaf and tuber was much more than that in stem and flower. The accumulation of 3GT transcripts in red2 pigmented callus was much more than that in non2pigmented callus of S. tuberosum cv. Chieftain. The expression of 3GT gene followed by anthocyanin accumulation.
LU Qi-neng
,
YANG Qing
,
SHEN Chun-xiu
. Cloning and Expression Analysis of Flavonoid 3-O-gulcosyltransferase Gene from Potato[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 11
-16
.
DOI: 10.7668/hbnxb.2009.04.003
[1] Tanaka Y,Katsumoto Y,Brugliera F,et al.Genetic engineering in floriculture[J].Plant Cell,Tissue and Organ Culture,2005,80:1-24.
[2] Schwinn K L,Davies K M,Deroles S C,et al.Expression of an Antirrhinum majus UDP-glucose:flavonoid-3-O-glucostransferase transgene alters flavonoid glycosylation and acylation in lisianthus (Eustoma grandiflorum Grise.)[J].Plant Sci99725:53-61.
[3] Yamazaki M,Yamagishi E,Gong Z,et al.Two flavoniod glucosyltransferases from Petunia Hybrida:molecular cloning,biochemical properties and developmentally regulated expression[J].Plant Mol Biol,2002,48:401-411.
[4] 孙明霞,王宝增,范海,等.叶片的花色素苷及其对植物适应环境的意义[J].植物生理学通讯,2003,39 (6):688-694.
[5] 张树军,李元,韩冰.矮牵牛的组织培养与快繁[J].内蒙古农业科技,2001(4):5-6.
[6] 高银.植物抗逆机制与基因工程研究进展[J].内蒙古农业科技,2007(5):75-78.
[7] 杨晓清,李正英,杨志华.荞麦皮黑色素提取条件及基本特性的研究[J].内蒙古农业科技,2000(6):17-18.
[8] 徐亚民,马越,赵晓燕,等.紫苏等4种天然色素抗氧化能力的比较[J].华北农学报,2007,22(2):187-190.
[9] Kobayashi S,Ishimaru M,Ding C K,et al.Comparison of UDP-glucose:Flavonoid 3-O-glucosyltransferase (UFGT)gene sequences between white grapes (Vitis Vinifera)and their sports with red skin[J].Plant Sci,200160:543-550.
[10] Afifi M,El-kdreamy A,Legrand V,et al.Control of anthocyanin biosynthesis pathway gene expression by eutypine,a toxin from Eutypa lata,in grape cell tiussue cultures[J].J Plant Physiol,200360:971-975.
[11] Nakatsuka T,Nishihara M,Mishiba K,et al.Two different muations are involved in the formation of white-flowered gentian plants[J].Plant Sci,200569:949-958.
[12] Holton T,Cornish E C.Genetics and biochemistry of anthocyanin biosynthesis[J].Plant Cell995,7:1071-1083.
[13] 卢其能,杨清,却志群,等.马铃薯CHS基因cDNA的克隆与表达分析[J].华北农学报,2008,23(5):17-22.
[14] Lu Q N,Yang Q.cDNA cloning and expression of anthocyanin biosynthetic genes in wild potato (Solanum pinnatisectum)[J].African Journal of Biotechnology,2006,5(10):811-818.
[15] De Jong W S,De Jong D M,De Jong H.An allele of dihydroflavonol 4-reductase associated with the ability to produce red anthocyanin pigments in potato (Solanum tuberosum L.)[J].Theor Appl Genet,200307:1375-1383.
[16] Jung C S,Griffiths H M,De Jong D M,et al.The potato plocus codes for flavonoid 3',5'-hydroxylase[J].Theor Appl Genet,200510:269-275.
[17] Knud L.Molecular cloning and characterization of cDNAs encoding cinnamoyl CoA reductase (CCR)from barley (Hordeum vulgare)and potato (Solanum tuberum)[J].J Plant Physiol,200461:105-112.
[18] Mancinelli A L,Rossi F,Moroni A.Cryptochrome,phytochrome,and anthocyanin production[J].Plant physiol991,96:1079-1085.
[19] Yamazaki M,Gong Z,Fukuchi-Mizutani M,et al.Molecular cloning and biochemical characterization of a novel anthocyanin 5-O-glucosyltransferase by mRNA differential display for plant forms regarding anthocyanin[J].J Biol Chem999,274:7405-7411.
[20] Dooner H K,Robins T P,Jorgensen R A.Genetic and developmental control of anthocyanin biosynthesis[J].Annu Rev Genet991,25:173-199.
[21] Hung C Y,Murray J R,Ohmann S O,et al.Anghocyanin accumulation during potato tuber development[J].J Amer Soc Hort Sci99722 (1):20-23.
[22] Honda C,Kotoda N,Wada M,et al.Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin[J].Plant Physiol Biochem,2002,40:955-962.
