In the paper,an indica variety Guangluai 4 and a japonica variety Taichung 65 were used to construct high generational backcross segregation population. Using 145 SSR markers which distributed on rice chromosomes,the polymorphism of parents and heading date of a BC3F2 mapping population were analyzed. The results showed that 114 markers displayed polymorphism between two parents,the rate of polymorphism is 78. 6 %; In BC3F1,the genetic back2 ground analysis detected ten SSR markers,which distributed on rice chromosome 2,3,4,7 and chromosome 10 ; In BC3F2 mapping population,the early2heading plants and late2heading plants segregated in a ratio of 146∶47,fitting the expected ratio of 3∶1,the former was 21 days earlier than the latter ;Linkage analyses suggested that the gene Ehd was tightly linked with SSR marker RM184 and RM271,the genetic distance between the two markers and Ehd were 216 cM and 211 cM,respectively. The mapping of the locus Ehd would be useful for its molecular marker2assisted selection.
ZHU Wen-yin
,
LI Wen-tao
,
DING Xiao-hua
,
ZHANG Ze-min
,
ZENG Rui-zhen
,
ZHU Hai-tao
,
ZHANG Gui-quan
. Mapping of the Dominant Earliness Gene Ehd Using SSR Markers in Rice[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(4)
: 7
-10
.
DOI: 10.7668/hbnxb.2009.04.002
[1] 水稻光温协作组.中国水稻品种的光温生态[M].北京:科学出版杜978:1-40.
[2] 董春林,孙业盈,邓晓建.水稻抽穗期基因研究进展[J].中国农学通报,2005,21(6):75-78.
[3] Li Z,Pinson S R M,Stansel J W,et al.Identification of two major genes and quantitative trait loci (QTLs)for heading date and plant height in cultivated rice (Oryza sativa L.)[J].Theor Appl Genet995,91:374-381.
[4] Yano M,Harushima Y,Nagamura,et al.Identification of quantitative trait loci controlling HD in rice using a high-density linkage map[J].Theor Appl Genet997,95:1025-1032.
[5] 邢永忠,徐才国,华金平,等.水稻株高和抽穗期基因的定位和分离[J].植物学报,2001,43(7):721-726.
[6] Yu S B,Li J X,Xu C G,et al.Identification for plant height and HD in rice[J].Theor Appl Genet,200204:619-625.
[7] Mei H W,Luo L J,Ying C S,et al.Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two testcross populations[J].Theor Appl Genet,200307:89-101.
[8] 何风华,席章营,曾瑞珍,等.利用单片段代换系定位水稻抽穗期QTL[J].中国农业科学,2005,38(8):1505-1513.
[9] 刘冠明,李文涛,曾瑞珍,等.水稻单片段代换系代换片段的QTL鉴定[J].遗传学报,2004,31(12):1395-1400.
[10] Tsai K H.Studies on earliness genes in rice,with special reference to analysis of isoalleles at the E locus[J].Japanese Journal of Genetics976,51(2):115-128.
[11] Tsai K H.Gene loci and alleles controlling the duration of basic vegetative growth of rice[C]//Rice Genetics.Philippines:International Rice Research Institute986:339-349.
[12] Tsai K H.Multiple alleles detected at the Ef-1 locus[J].Rice Genet Newsl9952:204-206.
[13] Sato S,Ogata K,Shinjyo C.Thermo-sensitive action of an earliness gene Ef-x in rice,Oryza sativa[J].Japanese Journal of Genetics992,67:473-482.
[14] 邓晓建,周开达,李仁端,等.水稻完全显性早熟性的发现和基因定位[J].中国农业科学,2001,34(3):233-239.
[15] Temnykh S,DeClerck G,Lukashova A,et al.Computational and experimental analysis of microsatellites in rice (Oryza sativa L.):Frequency,length variation,transposon associations,and genetic marker potential[J].Genome Res,20011:1441-1452.
[16] Zheng K L,Huang N,Bennett J,et al.PCR-based marker-assisted selection in rice seeding[M].IRRI Discussion Paper Series No.12.Internation Rice Research Institute,Philippines995.
[17] Ishii T,Brar D S,Multani D S,et al.Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice,Oryza sativa[J].Genome994,37:217-221.
[18] Wu K S,Tanksley S D.Abundance,polymorphism and genetic mapping of microsatellites in rice[J].Mol Gen Genet993,241:225-235.
[19] Doi K,Yoshimura A.RFLP mapping of a gene for heading date in an African rice[J].Rice Genet Newsl9985:148-149.
[20] Doi K,Izawa T,Fuse T,et al.Ehd1,a B-type response regulator in rice,confers shoRT-day promotion of flowering and controls FT-like gene expression independently of Hd1[J].Genes and Dev,20048:926-936.
