By measuring systematically in simulated chemical systems through the ways of scavenging DPPH,ABTS and superoxide anion radicals and FRAP method,the antioxidant activity of acetone-extracting from kiwifruits pulp with flash extraction and the content of the total phenol and total flavonoids of the extract were measured. The results showed that the content of total phenol and total flavonoids of the extract were(3. 19 ± 0.53)mg/g and(0.26 ±0.07)mg/g,respectively. The research in simulated chemical systems showed that the acetone-extracting from kiwifruits hadgreat scavenging effect against·OH,DPPH·andO2-·in the experimental range. When the con-centrate of sample was 1g/mL,the scavenging rate to superoxide anion radicals was up to 90.7%. And when the concentrate was 2g/mL,the scavenging rate to ·OH and DPPH· was 84.5% and 82. 2%,respectively. With the increasing concentration,the scavenging effect enhanced,which suggested the presence of dose-effect relations. Be-side,the FRAP value of acetone-extracting from kiwifruits also increased with concentration,which had excellent significant correlation with its DPPH· andO2-·radical-scavenging activity,significant correlation with its·OH rad-ical-scavenging activity. The results provide the theory basic for kiwifruits exploitation.
WANG Hua
,
CAO Jing
,
ZHAI Li-juan
,
XUE Zhao-hui
. Investigation on the Anti-oxidative Activity of Kiwifruits Pulp Extracts[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(2)
: 144
-149
.
DOI: 10.3969/j.issn.1000-7091.2013.02.026
[1] Valko M,Leibfritz D,Moncol J,et al. Free radicals and antioxidants in normal physiological functions and human disease[J]. International Journal of Biochemistry ＆ Cell Biology,2007,39(1):44-84. 2 期王华等: 猕猴桃果肉提取物抗氧化活性研究 149
[2] 杨艳杰,白新鹏,裘爱泳. 猕猴桃属植物的研究进展
[J]. 安徽农业科学,2007,35(35):11454-11457.
[3] 王建东,杨 静.京津地区猕猴桃栽培管理技术[J]. 天津农业科学,2010,16(3):144-146.
[4] 吴 玉.猕猴桃研究慨况[J]. 上海医药,2010,31(4): 187-188.
[5] 宋圃菊. 中华猕猴桃的防癌作用(五)阻断大鼠和健康 人体内 N-亚硝基脯氨酸的合成[J]. 营养学报,1988,10(1):50.
[6] 杨艳杰,何弘水. 猕猴桃根提取物体外抗氧化活性研究[J]. 安徽农业科学,2011,39(29):17773-17774.
[7] Wolfe K,Wu X Z,Liu R H. Antioxidant activity of apple peels[J]. Agricultural and Food Chemistry,2003,51(3): 609-614.
[8] 孙波,Bekhita E D,王坤波,等.不同品种蓝莓提取物抗氧化作用的研究 [J]. 食品科学,2007,28(10):61-63.
[9] 吴亮亮,石雪萍,张卫明. 花椒总黄酮测定方法研究
[J]. 食品工业科技,2010,10(3):372-374.
[10] Halliwell B,Gutteridge J M C,Aruoma O I. The deox-cyribose method:A simple 'test-tube' assay for determi-nation of rate constants for reactions of hydroxyi radials
[J]. Analytical biochemistry,1987,165:215-219.
[11] Brandwilliams W,Cuvelier M E,Berset C. Use of a free-radical method to evaluate antioxidant activity[J]. Food Science and Technology-Lebensmittel-Wissenschaft and Technologie,1995,28(1):25-30.
[12] 贾之慎,杨贤强.茶多酚清除活性氧自由基 O2－·和 ·OH的分光光度法研究[J]. 中国茶叶,1993,19 (1):25.
[13] Benzie I F F,Strain J J. The Ferric reducing ability of plasma(FRAP) as a measure of antioxidant power:The FRAP assay[J]. Analytical Biochemistry,1996,239 (1):70-76.
[14] 文镜,贺素华,张博成,等.红景天提取物清除 O2－· 和·OH 的体外实验研究[J]. 食品科学,2005,26 (2):121-124.
[15] 李慎新,曹新志,钟俊波,等.几种中草药抗氧化性成分的还原能力及总酚含量比较研究[J]. 安徽农业科学,2008,36(29):12755-12756.
[16] Lebeau J,Furman C,Bemier J L,et al. Aniioxidant prop-erties of di-tert-butylhydroXylated flavonoids[J]. Free Radical Biology ＆ Medicine,2000,29(9):900-912.
[17] Owen R W,Haubner R,Mier W,et al. Isolation,struc-ture elucidation and antioxidant potential of the major phenolic and flavonoid compounds in brined olive drupes
[J]. Food and Chemical Toxicology,2003,41 (5): 703-717.
[18] Whang J I,Moon H I,Zee O P. Phytochemical constitu-ents of Aetinidia arguta[J]. Korean Journal of Pharma-cognosy,2000,31(3):357-363.
[19] Webby R F. A flavonol triglycoside from Actinidia arguta var.giraldii[J]. Phytochemisry,1991,30(7):2443-2444.
[20] 杨少辉,宋英今,王洁华,等. 雪莲果体外抗氧化和自由基清除能力[J]. 食品科学,2010,31(17):166-170.
[21] 李鹏,李萍,田向学,等. 丹参提取剩余物的体外 抗氧化活性研究[J]. 天津农业科学,2011,17(5):50-52.
[22] 朱尚彬,聂少平,朱盼,等. 黑灵芝不同溶剂提取物抗氧化活性比较研究[J]. 食品科学,2009,30(17): 98-101.
[23] 赵金伟,李范洙,张先.苹果梨酚类物质抗氧化活性研究[J]. 食品科学,2010,31(17):170-173.
