In this paper,the 5-year oldgrapevines of Vitis vinifera L. Cabernet Sauvignon were subjected to reg-ular UV-C irradiation,and the effect of UV-C on the fruit quality formation and accumulation of polyphenols during berry development were studied. The results showed that the pattern of fruit quality formation and accumulation of polyphenols were not changed,but the fruit quality and content of polyphenols in mature berry were changed signifi-cantly by the regular UV-C irradiation. Under the moderate UV-C irradiation,no obvious changes in single berry weight,content of organic acid,and total phenol were observed,but the content of anthocyanins,total sugar were in-creased,and the accumulation of flavonoids and flavanols were induced significantly in mature berry,which sugges-ted that there was obvious improving in fruit quality. In addition,the effect of UV-C on the fruit quality formation and accumulation of polyphenols were development and dose-dependent.
[1] 温鹏飞.葡萄多酚[M]. 北京:中国农业科学技术出版社,2012.
[2] Monagas M,Gómez-Cordovés C,Bartolome B,et al. Mon-omeric,oligomeric,and polymeric flavan-3-ol composition of wines andgrapes from Vitis vinifera L. cv.graciano,Tempranillo,and Cabernet Sauvignon[J]. Journal of Agri-cultural and Food Chemistry,2003,51:6475-6481.
[3] Zimman A,Joslin W S,Lyon M L,et al. Maceration varia-bles affecting phenolic composition in commercial-scale Cabernet Sauvignon winemaking trials [J]. American Journal of Enology and Viticulture,2002,53:93-98.
[4] Vidal S,Cartalade D,Souquet J M,et al. Changes in proanthocyanidins chain in wine-like model solutions[J]. Journal of Agricultural and Food Chemistry,2002,50: 2261-2266.
[5] Girona J,Marsal J,Mata M,et al. Phenological sensitivity of berrygrowth and composition of Tempranillograpevines (Vitis vinifera L. )to water stress[J]. Australian Journal ofgrape and Wine Research,2009,15:268-277.
[6] 温鹏飞.葡萄与葡萄酒中黄烷醇类多酚和果实原花色素合成相关酶表达规律的研究[D]. 北京:中国农业大学,2005.
[7] Dixon R A,Xie D Y,Sharma S B. Proanthocyanidins-a fi-nal frontier in flavonoid research[J]. New Phytologist,2005,165:9-28.
[8] 吴鲁阳,张振文,贺猷.紫外线-B辐射增强对葡萄植株形态的影响[J].干旱地区农业研究,2006,24(4): 87-89.
[9] 孙莹,张振文,张景昱,等.紫外线-B辐射增强对葡萄浆果的影响[J].干旱地区农业研究,2010,28(1): 164-167,205.
[10] Sun M,Gu X,Fu H,et al. Change of secondary metabo-lites in leaves ofginkgo biloba L. in response to UV-B induction[J]. Innovative Food Science and Emerging Technologies,2010,11:672-676.
[11] 孙莹,张振文,岳泰新,等.不同强度紫外线UV-B辐射对葡萄品质影响[J].中国酿造,2009,211(10): 15-18.
[12] Interdonato R,Rosa M,Nieva C B,et al. Effects of low UV-B doses on the accumulation of UV-B absorbing compounds and total phenolics and carbohydrate metabo-lism in the peel of harvest lemons[J]. Environmental and Experimental Botany,2011,70:204-211.
[13] Zhang Z Z,Li X X,Chu Y N,et al. Three types of ultra-violet irradiation differentially promote expression of shikimate pathwaygenes and production of anthocyanins ingrape berries[J]. Plant Physiology and Biochemistry,2012,57:74-83.
[14] Wang W,Tang K,Yang H R,et al. Distribution of res-veratrol and stilbene synthase in younggrape plants(Vi-tis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulation[J]. Plant Physiology and Bio-chemistry,2010,48:142-152.
[15] Liu W,Liu C Y,Yang C X,et al. Effect ofgrapegeno-type and tissue type on callusgrowth and production of resveratrols and their piceids after UV-C irradiation[J]. Food Chemistry,2010,122:475-481.
[16] 温鹏飞,邢延富,牛铁泉,等. UV-C对葡萄果实发育过程中黄烷醇类多酚积累及隐色花色素还原酶表达的影响[J]. 中国农业科学,2012,45(21):4428-4436.
[17] Guerrero R F,Puertas B,Fernández M I,et al. Induction of stilbenes ingrapes by UV-C:Comparison of different subspecies of Vitis[J]. Innovative Food Science and E-merging Technologies,2010,11:231-238.
[18] González-Aguilarg A，Zabaleta-Gatica R，Tiznado-Hernández M E. Improving postharvest quality of mango ‘Haden’by UV-C treatment[J]. Postharvest Biology and Technology,2007,45:108-116.
[19] 蔡少芳,江海燕,欧艳萍.山楂不同泡制品有机酸含量测定[J].中成药,2000,22(12):841-842.
[20] 丁礼琴,刘力,徐德生.蒽酮-硫酸法测定生地黄提取物中总糖的含量[J]. 上海医药,2008,29(8): 368-370.
[21] 高俊凤,孙群,曹翠玲,等.植物生理学实验指导
[M].北京:高等教育出版社,2006.
[22] Ivanova V,Stefova M,Vojnoski B,et al. Identification of polyphenolic compounds in red and whitegrape varietiesgrown in R. Macedonia and changes of their content dur-ing ripening[J]. Food Research International,2011,44: 2851-2860.
[23] 温鹏飞,郑宏佳,牛铁泉,等.延迟采收对葡萄果实多酚类物质含量的影响[J]. 山西农业大学学报:自然科学版,2011,31(5):446-450.
[24] 田莉.葡萄果实查耳酮合成酶研究[D]. 北京:中国农业大学,2007.
[25] 贺艳楠,张振文.紫外线辐射对葡萄生长发育的影响
[J]. 北方园艺,2012(4):182-185.
[26] 厉维江,厉超阳,谢亚利,等.采后UV-C处理对葡萄抗病性和品质的影响[J]. 安徽农业科学,2011，39 (9):5351-5353.
[27] Degaulejac N S C,Glories Y,Vivas N. Free radical scavenging effect of anthocyanins in red wines[J]. Food Research International,1999,32:327-333.
[28] 李海平,张惠,李灵芝,等.镧对UV-C胁迫下番茄幼苗叶绿素含量的影响[J]. 山西农业科学,2011,39 (2):33-35.
[29] 周新明,张振文,惠竹梅,等.UV-B辐射增强对葡萄光合作用日变化的影响[J]. 农业工程学报,2009,25 (3):209-212.
[30] 吴业飞,吴鲁阳,张振文.紫外线-B辐射增强对葡萄叶片抗氧化系统的影响[J]. 西北农林科技大学学报:自然科学版,2008,36(12):161-166.
[31] 王文刚,李灵芝,张惠,等.镧对UV-C胁迫下番茄幼苗抗氧化酶活性的影响[J]. 山西农业科学,2011,39(6):46-48,56.
[32] Tegelberg R,Julkunen-Tiitto R,Aphalo P J.The effects of ling-term elevated UV-B on thegrowth and phenolics of field-grown silver birch(Betula pendula)[J].global Change Biology,2001,34:71-89.
