In this study,a Chinese Citrus tristeza virus( CTV) isolate HB1 showing unusual serological characteristic was identified using three antibodies from different sources( PAb-L5,PAb-I,MAbs- S4) in Wuhan,Hubei province. CTV- HB1 could only be recognized by a polyclonal antibody( PAb-L5) raised against a Chinese isolate L5 in both ELISA and DTBIA. To understand the molecular mechanisms underlying such anomalous serological property, the sequences of CP gene were identified for CTV- HB1 and other isolates. Comparison of the CP gene sequences from CTV- HB1with those of other 19 isolates published in GenBank revealed that the sequence similarity was 90. 4 %to 99. 4%at nucleotide level. However,only three amino acid residues in the CP gene from CTV-HB1were different from that of CTV-L5. All the above results suggested that the three changed position( s) might be correlated to the serological property of CTV- HB1
WANG Cai xia
. The Study of Serological Property of CP Gene From a Chinese Citrus Tristeza Virus Isolate HB1[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 212
-214
.
DOI: 10.7668/hbnxb.2009.03.044
[1] Bar-Joseph M,Marcus R,Lee R F.The continuous challenge of citrus tristeza virus control[J].Annu Rev Phytopatho,1989,27:291-316.
[2] Permar T A,Garnsey S M,Compf D J,et al.A monoclonal antibody that discriminate strains of citrus tristeza virus[J].Phytopathology,1990,80:224-228.
[3] Nikolaeva O V,Karasev A V,Powell C A,et al.Mapping of epitopes for citrus tfisteza virus-specific monoclonal,antibodies using bacterially expressed coat protein fragments[J].Phy-topathology,1996,86:974-979.
[4] Nikolaaeva O V,Karasev A V.Serological differentiation of the citrus,tristeza virusisolates causing stem pitting in sweet orange[J].Plant Dis,1998,82:1276-1280.
[5] 王彩霞,王国平,姜波,等.柑橘衰退病毒多克隆和单克隆抗体的制备及检测效果分析[J].生物工程学报,2006,22(4):629-634.
[6] Karasev A V,Boyko V P,Cowda S,et al.Complete sequence of the citrus tristeza virus RNA genome[J].Virology,1995,208:511-520.
