The rice cultivars and lines which attended regional trials and represented the current situation of rice breeding in Northeast Region of China were selected as material to analyze genetic diversity and genetic relationship be2 tween different provinces and cultivars on rice by SSR markers. As a result,only 303 alleles were detected in 103 poly2 morphic loci with the average number of 2194 and the distribution of alleles was uneven. So the genetic diversityof rice in Northeast Region of China was narrow,significantly lower than others rice regions. The analysis of genetic diversity be2 tween different chromosomes showed that the polymorphic loci distribution of Chr. 6,Chr. 9 and Chr. 11 was evener and the polymorphism information content was also more than others chromosomes. The homozygosity of three provinces,Hei2 longjiang was highest,Jilin was secondary and Liaoning was lowest. The degree of genetic diversity was in order of Hei2 longjiang >Jilin >Liaoning. The result of genetic cluster analysis indicated that there was the closer genetic relationship between Heilongjiang and Jilin,correspondingly Liaoning was more different.
CHEN Ying hua
,
LI Hong yu
,
HoU Yu ming
,
XU Zheng jin
,
YUAN Yuan
,
LI Mao bai
. Genetic Diversity of Rice Germplasm Resources in Northeast Region of China[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 165
-173
.
DOI: 10.7668/hbnxb.2009.03.036
[1] 徐正进,李雪梅,张文忠.杂交粳稻后代经济性状变化规律及其相关性研究[J].沈阳农业大学学报,2003,34(5):328-331.
[2] 邱福林,庄杰云,华泽田.北方杂交粳稻骨干亲本遗传差异的SSR标记检测[J].中国水稻科学,2005,19(2):101-10.
[3] 夏铭.遗传多样性研究进展[J].生态学杂志,1999,18(3):59-65.
[4] 程本义,施勇烽,沈伟峰.南方稻区国家水稻区域试验品种的微卫星标记分析[J].中国水稻科学,2007,21(1):7-12.
[5] 王一平,魏兴华,华蕾.不同地理来源早稻种质资源的遗传多样性[J].作物学报,2007,12:2034-2040.
[6] 陈先红,徐利远,彭正松.中国西南地区小麦品种(系)遗传多样性的SSR分析[J].麦类作物学报,2008,28(1):6-1.
[7] 黄朝锋.水稻PSM标记的发展及抗虫基因的分子定位[D].广州:华南农业大学,2003.
[8] Murray M G,Thompson W F.Rapid isolation of high molecu-lar weight plant DNA[J].Nucl Acids Res,1980,8:4321-4325.
[9] Panaud O,Chen X,McCouch S R.Development of a mi-cresatellite markers and characterization of simple sequence length polymorphism(SSLP)in rice(O.sativa L.)[J].Mol Gen Genet,1996,252:597-607.
[10] Yeh FC,Yang R.Popgene v 1.32.http:∥www.ualberta.ca,2003.
[11] Nei M.Moheular Evolutionary Genetics[M].New York:Columbia University Press,1987.
[12] Shannon C E.A mathematical theory of communication[J].Bell Syst Technol,1948,27:379-423.
[13] Wright S.Variability within and among natural populations[M].Vol 4.Chicago:The Univ of Clucago Press,1978.
[14] Hard D L,Clark A G.Principles of population genetics.2nd ed.Sinauer Associates,Sunderland,MA,1989.
[15] Excoitier L,Laval L G,Schneider S.Arlequin ver.3.0:An integrated software package for population genetics data analysis[J].Evol Bioinform Online,2005,1:47-50.
[16] Rohlf F J.NTSYSpc:Numerical taxonomy and multivariate analysis system[M]∥Exeter Software,Applied Biostafstics Inc New York,USA,2000.
[17] 南宫自艳,高宝嘉,刘军侠.四种松毛虫不同地理种群遗传多样性的等位酶分析[J].昆虫学报,2008,51(4):417-423.
[18] Hamriek J L,Godt M J W.Allozyme diversity in plant species[M]//Brown A H D,Clegg M T,Kaher A L,et al.Plant population genetics,breeding and genetic resources.Sunderland:Sinauer,1989:43-63.
[19] 王一平,魏兴华,袁筱萍.海南普通野生稻自然居群间遗传多样性的微卫星分析[J].中国水稻科学,2007,21(6):573-578.
[20] Nei M.Genetic distance between populations[J].Am Nat,1972,106:283-292.
[21] Nei M.Estimation of average heterozygosity and genetic dis-tance from a smallnumber of individuals[J].Genetics,1978,89:583-590.
[22] 齐永文,张冬玲,张洪亮.中国水稻选育品种遗传多样性及其近50年变化趋势[J].科学通报,2006,51:693-699.
[23] 熊振民,蔡洪法.中国水稻[M].北京:中国农业出版社,1990:50-200.
[24] Mackill D J.Classifying japonica rice cultivars with BAPD makers[J].Crop Sci,1995,35:889-894.
