The normal and vitrified plantlets of Isatis indigotica Fort. cultured in vitro were experiment materials, the changes of activitities of antioxidant enzyme and stress by oxygen free radicals were investigated. The results showed that:in the course of culture of Isatis indigotica Fort. shoots, the activity of Peroxidase( POD) in both normal and vitrified shoots increased at first and then decreased.The activity of Catalase( CAT) decreased at first and then increased.The activity of Superoxide dismutase(SOD) andMalondiadehyde ( MDA) content were gradually increased. The activities of CAT and SOD in vitrified shoots were lower than those in normal shoots, but MDA levels in vitrified shoots was higher than that in nor - mal shoots. It can be inferred that the function of protective enzymes of vitrified shoots was abnormal, so the vitrified shoots were stressed and destroyedby oxygen free radicals more heavily than normal shoots. In addition, the belts of Perox - idase isozymes of vitrified shoots lost or added compared with normal shoots. This result indicated that the expression of isozymes was abnormal and the hereditary stability of vitrified shoots have changed.
KE Shao ying
,
ZHANG Sheng zhen
,
LIU Yu jun
. The Changes of Antioxidant Enzymes Activity of Isatis indigotica Fort.during Vitrification in vitro[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 154
-158
.
DOI: 10.7668/hbnxb.2009.03.033
[1] 崔树玉,薛原,杨建莉.板蓝根研究进展[J].中草药,2001,32(7):670-671.
[2] 梁海曼,周菊华.试管苗玻璃化现象的生理生化和机理探讨[J].武汉植物学研究,1994,12(3):281-286.
[3] 李娅莉,张健,播远智.观赏植物组织培养过程中玻璃化现象与解决措施进展[J].四川农业大学学报,2004,22(3):278-280.
[4] 韦梅琴,唐蓉,沈宁东,等.香石竹试管正常苗和玻璃化苗茎叶的解剖特征比较[J].青海大学学报:自然科学版,1997,15(1):18-21.
[5] 李云,田砚亭,罗小芳.玻璃苗中纤维素、木素及元素含量变化的研究[J].农业学报,1997,11(1):103-111.
[6] 贾炜珑,周小梅,杨丽莉,等.康乃馨玻璃苗生理生化探讨[J].山西大学学报:自然科学版,1997,20(2):228-231.
[7] 赵剑,杨文杰.梨芽离体快繁过程中玻璃苗的发生与氧自由基胁迫的关系[J].西北植物学报,1998,18(2):183-189.
[8] 王纪方,李锡香,贾春兰.丝石竹玻璃苗生理特性和形成机理初探[J].农业生物技术学报,1997,1(5):72-78.
[9] 谢芝馨,张玉喜,于元杰,等.大葱试管苗玻璃化机理的探讨[J].分子植物育种,2004,2(1):71-75.
[10] 李云,田砚挺,罗晓芳.珠美海棠试管苗玻璃化发生机理的初步研究[J].北京林业大学学报,1996,18(1):52-58.
[11] 王学奎,张文华,郝再彬.植物生理生化实验原理和技术[M].第2版.北京:高等教育出版社,2006:167-182,270-271.
[12] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社.2000:260-261.
[13] Chen Zu-liang,Zhu Yong-guan.capillary gel electrophoretic separation of superoxide dismulase in leaf extracts of Triticum aestivum L[J].Phytochemical Analysis,2000,11:362-365.
[14] Veneta Kapchina-Toteva,Elena Yakimova.Effect of purine and phenylurea cytokinins on peroxidase activity in relation to apical dominance of in vitro cultivated Rosa hybrida L.[J].Bulg J Plant Physiol,1997,23(1-2):40-48.
[15] 李胜,李唯,杨德龙,等.植物试管苗玻璃化现象研究进展[J].甘肃农业大学学报,2003,3(1):1-16.
[16] 孙国荣,彭永臻,阎秀峰,等.干旱胁迫对白桦实生苗保护酶活性及脂质过氧化作用的影响[J].林业科学,2003,39(1):165-167.
[17] 宋关玲,侯文华,汪群慧,等.低温对青萍生长及保护酶活性影响的研究[J].环境科学研究,2004,17(增刊):45-48.
[18] 武敬亮,田桂香,孙敏.盐碱胁迫和SNP对黄连膜脂过氧化及保护酶活性的影响[J].浙江林业科技,2006,26(5):5-10.
[19] 田国忠,李怀方,裘维蕃.植物过氧化物酶研究进展[J].武汉植物学研究,2001,19(4):332-344.
[20] 张淑改,齐力旺,时宝凌,等.满天星玻璃苗的酯酶和过氧化物酶研究[J].山西农业科学,1999,27(2):68-70.
