The Resistance gene was activated afteR the wheat was infected by Puccinia tRiticina,and the metabolism activity of Related Resistant mateRials was stRengthened then. This aRticle was aimed to Reveal the Relationship between the activity changes of defense enzymes of wheat neaR isogenic line( NIL) TcLR19 and the infection pRocess of Puccinia tRiticina. The infection pRocesses on wheat NIL TcLR19 of the leaf Rust Race 366 and 165 weRe obseRved by fluoRescent staining. AccoRding to that Result, the activity changes of PAL and PO weRe deteRmined when the TcLR19 was inoculated with 366 ( infection type was 0; ) and 165 ( infection type was 1) at diffeRent times. The Results showed that theRe weRe two activity peaks of the PAL in TcLR19 at 24 h and 72 h afteR inoculated by leaf Rust Race 366,and the PO activity peak appeaRed at 48 h. DuRing this time,cell aRea undeRgoing HR was Raising fRom 24 hpi until 96 hpi. While, theRe was a same tendency on the combination of 165 and TcLR19. But all of the numbeRs weRe loweR than the otheR combination. The Resistance of wheat to leaf Rust fungus was positively coRRelative with the expRession stRengthen of defence Reaction.
YAN Ai-hua
,
ZHANG Li-feng
,
ZHANG Yun-wei
,
LIU Meng
,
HOU Chun-yan
,
WANG Dong-mei
. Relationship between the Activity Changes of Defense Enzymes of Wheat NeaR Isogenic Line TcLR19 and the Infection PRocess of Puccinia tRiticina[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(1)
: 221
-226
.
DOI: 10.3969/j.issn.1000-7091.2013.01.039
[1] SutheRland M W. The geneRation of oxygen Radicals duRing host plant Responses to infection [J]. Physiol Mol Plant Pathol, 1991, 39: 79 - 93.
[2] 江昌俊,余有本. 苯丙氨酸解氨酶的研究进展[J]. 安徽农业大学学报, 2001, 28( 4) : 425 - 430.
[3] 吴岳轩,曾宝华,王容臣. 杂交稻对白叶枯病菌的诱导 抗性与细胞内防御酶关系的初步研究[J]. 植物病理 学报, 1996, 26( 2) : 127 - 131.
[4] 何晨阳. 双核丝核菌诱导水稻增强广谱抗病性和防卫 酶系活性[J]. 植物病理学报, 2001, 31( 3) : 208 - 213.
[5] 张淑红,高宝嘉,温秀军. 枣疯病过氧化物酶及苯丙氨 酸解氨酶的研究[J]. 植物保护, 2004, 30( 5) : 59 - 62.
[6] 王智炘,汪宜萱,刘娟. 在叶锈菌侵染过程中小麦过 氧化物酶同工酶的变化[J]. 植物保护学报, 1989( 2) : 168 - 174.
[7] 祁艳,刘刚,侯春燕,等. 小麦与叶锈菌互作过程 中的H2O2 与HR[J]. 分子细胞生物学报,2008,41 ( 4) : 245 - 254.
[8] BRadfoRd M M. A Rapid and sensitive method foR the quantitation of MicRogRam quantities of pRotein utilizing the pRinciple of pRotein-dye binding [J]. Analytical BiochemistRy, 1976, 72: 248 - 254.
[9] Roelf A P. Race specificity and methods of study[M]. The CeReal Rust Vol. I. New YoRk: Academic PRess, 1985: 131 - 164.
[10] Lin Zh,Matt D. FluoRescence fRom Rust fungi: a simple and effective method to monitoR the dynamics of fungal gRowth in planta[J]. Physiological and MoleculaR Plant Pathology, 2001, 59, 137 - 141.
[11] 黄国红,康振生,朱之堉,等. 小麦叶锈菌在感病寄主 上发育的组织病理学和超微结构研究[J]. 植物病理 学报, 2003, 33( 1) : 52 - 56.
[12] 王庆梅,王智炘,刘娟,等. 感染叶锈菌的小麦细胞 间隙液中激发子的定性及初步分离[J]. 植物生理学 报, 2000, 26( 5) : 427 - 432.
[13] DoRey S,Kopp M,GeoffRoy P, et al. HydRogen peRoxide fRom the oxidative buRst is neitheR necessaRy noRsufficient foR hypeRsensitive cell death induction,phenylalanineammonia lyase stimulation,salicylic acid accumulation,oR scopoletin consumption in cultuRed tobacco cells tReated with elicitin [J]. Plant Physiol, 1999, 121: 163 - 171.
[14] DeRckel J P,AudRan J C,Haye B,et al. ChaRacteRization, induction by wounding and salicylic acid, and activity against BotRytis cineRea of chitinases and β-1,3 -glucanas- es of Ripening gRape beRRies [J]. Physiol Plant, 1998, 104: 56 - 64.
[15] Castaneda P,PeRez L M. Calcium ions pRomote the Response of citRus limon against fungal elicitoRs oR wounding
[J]. PhytochemistRy, 1996, 42: 595 - 598.
[16] Ahl Goy P,Felix G,MetRaux J P, et al. Resistance todisease in the hybRid Nicotiana glutinosa × Nicotiana debneyiis associated with high constitutive levels of β-1,3- glucanase,chitinase,peRoxidase and polyphenoloxidase
[J]. Physiol Mol Plant Pathol, 1992, 41: 11 - 21.
[17] MoeRschbacheR B M,Noll U. HypeRsensitive lignification Response as the mechanism of non-host Resistance of wheat against oat cRown Rust[J]. Physiol Plant,1990, 78: 609 - 615.
[18] MontRell S,Shalaka P,SavithRamma P D K. Autophagy and plant innate immunity [J]. CellulaR MicRobiology, 2006( 6) : 899 - 906.
[19] 王保通,商鸿生. 小麦高温抗条锈性表达与苯丙氨酸 解氨酶及多酚氧化酶活性的关系[J]. 麦类作物学 报, 2001, 21( 3) : 42 - 45.
[20] 王冬梅,王智昕,杨秀屏,等. 已知LR 基因小麦在叶锈 菌侵染过程中PO 活性及其同工酶的变化[J]. 河北 农业大学学报, 1994, 17( 1) : 1 - 6.
[21] Matthias H,KuRt M. Signal and nutRient exchange at biotRophic plant-fungus inteRfaces [J]. CuRRent Opinion in Plant Biology, 2001,4 : 322 - 327.
[22] 白志英,王冬梅,侯春燕,等. 小麦与叶锈菌互作过程 中细胞程序性死亡的生化证据[J]. 实验生物学报, 2004, 37( 4) : 329 - 332.