Part of conservative sequence of BADH from Puccinellia chinampoensis has been already known and was used as the basis of the research when specific primer was designed. The BADH gene of Puccinellia chinampoensis was cloned by SMART RACE. These two fragments were assembled and the result showed that we obtained the 3′ end and part of 5′ end. The sequence was 1 502 bp in length,its nucleotide sequence share 89 % with the cDNA of BADH from Ley2 mus chinensis and Hordeum brevisubulatum. Its deduced amino acid sequence contains the conserved domain sequence VT LELGGKSP of BADH and the Cys associated with aldehyde dehydrogenase function.
ZHoNG Ming
,
ZHANG Jia
,
GUo Zhi fu
,
ZHANG Li
,
WANG Lei
. The Amplification of BADH Gene 3'and 5'End from Puccinellia chinampoensis[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 46
-50
.
DOI: 10.7668/hbnxb.2009.03.010
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