Part of conservative sequence of BADH from Puccinellia chinampoensis has been already known and was used as the basis of the research when specific primer was designed. The BADH gene of Puccinellia chinampoensis was cloned by SMART RACE. These two fragments were assembled and the result showed that we obtained the 3′ end and part of 5′ end. The sequence was 1 502 bp in length,its nucleotide sequence share 89 % with the cDNA of BADH from Ley2 mus chinensis and Hordeum brevisubulatum. Its deduced amino acid sequence contains the conserved domain sequence VT LELGGKSP of BADH and the Cys associated with aldehyde dehydrogenase function.
ZHoNG Ming
,
ZHANG Jia
,
GUo Zhi fu
,
ZHANG Li
,
WANG Lei
. The Amplification of BADH Gene 3'and 5'End from Puccinellia chinampoensis[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(3)
: 46
-50
.
DOI: 10.7668/hbnxb.2009.03.010
[1] 杨铮,钟鸣,郭志富,等.盐胁迫下朝鲜碱茅的甜菜碱醛脱氢酶活性变化及其基因保守区的克隆[J].植物生理学通讯,2007,43(3):430-434.
[2] MeCue K F,Hanson A D.Salt.inducible betaine aidehyde de-hydrogenase from sugar beet:eDNA cloning and expression[J].Plant Mol Biol,1992,18:1-11.
[3] Li Q L,Gao X R,Yu X H,et al.Molecular cloning and char-acterizafion of betaine aldehyde dehydrogenase gene from Suaeda liaotangens and its use in improved tolerance to salini-ty in transgenic tobacco[J].Biotechnol Lett,2003,25(17):1431-1436.
[4] Yin X J,Zhao Y X,Luo D,et al.Expression of the betaine aldehyde dehydrogenase(AcBADH)gene and isolation otits promoter from the halophyte Ataplex eentralasiafica[J].Jour-nal of Plant Physiology and Molecular Biology,2002,28(6):479-484.
[5] Legaria J,Rajsbaum R,Mufioz-Clares R A,et al.Molecular characterization of two genes encoding betaine aldehyde dehy-drogenase from amaranth expression in leaves under short-term exposure to osmotic stress or abscisic acid[J].Gene,1998.218:69-76.
[6] Hibino T,Meng Y L,Kawamitsu Y,et al.Molecular cloning and function characterization of two kinds of betaine aldehyde dehydrogenase in betaine-accumulating man grove Avicennia marina(Forsk.)Vierh[J].Plant Mol Bid,2001,45:353-363.
[7] Ishitani M,Nakamum T,Hart S Y,et al.Expression ofthe be-taine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid[J].Plant Mol Biol,1995,27:307-315.
[8] Nakamura T,Nomura M,M ori H,et al.An isozyme of be-taine aldehyde dehydrogenase in barley[J].Plant Cell Physi-ol,2001,42(10):1088-1092.
[9] Wood A J,San eoka H,Rhodes D.et al.Betaine aldehyde dehydrogenase in sorghum molecular cloning and expression of two related genes[J].Plant Physial,1996,110:1301-13018.
[10] Nakamura T,Yokota S,M uramoto Y,et al.Expression of a betaine Mdehyde dehydrogenase gene in rice,a glycinebe-taine Nonaccumulator,and possible localization of its protein in peroxisomes[J].Plant J,1997,11(5):1115-1120.
[11] Li Y H,Zou Q.The sequence of Triticum oestivwn betaine aldehyde dehydrogenase gene WBADH[J].Journal of Plant Physiology and Molecular Biology,2002,28(6):495-496.
[12] Rathinasabathi B,McCue K F,Gago D A,et al.Metabolic engineering of glycine betaine synthesis:plant betaine aide-hyde dehydrogenase lacking typical transit peptides are tar-geted to tobacco chloroplasts where they confer betaine alde-hyde resistance[J].Planta,1994,193:155-162.
[13] 梁峥,马德钦,汤岚,等.菠菜甜菜碱醛脱氢酶基因在烟草中的表达[J].生物工程学报,1997,3(3):236-240.
[14] 刘风华,郭岩,谷冬梅,等.转甜菜碱醛脱氢酶基因植物的耐盐性研究[J].遗传学报,1997,24(1):54-58.
[17] 肖岗,张耕耘,刘风华,等.山菠菜甜菜碱醛脱氢酶基因研究[J].科学通报,1995,40(8):741-745.
[16] 董海滨,管荣展,张红生,等.播娘嵩甜菜碱醛脱氢酶基因的克隆和表达分析[J].分子植物育种,2006,4(2):209-215.
[17] 曾幼玲,幸婷,蔡忠贞,等.盐生植物盐爪爪甜菜碱醛脱氢酶基因的克隆及在盐胁迫下的BADH基因的表达[J].云南植物研究,2007,29(1):79-84.
[18] 刘振林,戴思兰.植物甜菜碱醛脱氢酶基因研究进展[J].西北农林科技大学学报:自然科学版,2004,32(3):10一112.
[19] 崔杰,李滨胜,史淑芝,等.植物甜菜碱醛脱氢酶基因工程研究进展[J].中国甜菜糖业,2006(1):40-44.