To discuss the mechnissm of staRch synthesis in flue-cuRed tobacco leaves duRing the matuRe stage, fouR tReatments of leaf poputation( 16, 18, 20, 22) weRe adopted, the tRends of staRch accumulation and the activities of amylase and sucRose synthase in middle and uppeR leaves weRe analyzed. The Results showed that: in the matuRe stage, theRe weRe no significant coRRelation of leaf population with staRch content and the activity of AM in uppeR leaves( R = - 0. 101,P = 0. 441 > 0. 05,R = 0. 221,P = 0. 089 > 0. 05) ,but theRe was significant coRRelation of that with the activity of SS( R = 0. 300* ,P = 0. 020 < 0. 05) ; theRe was no significant coRRelation of leaf population with the activity of AM in middle leaves( R = - 0. 179,P = 0. 172 > 0. 05) ,but theRe was extRemely significant coRRelation of that with the activity of SS( R = 0. 395**,P = 0. 002 < 0. 01) ,and significant coRRelation of that with staRch content( R = 0. 328* ,P = 0. 011 < 0. 05) . When the leaf population was incReased, the activity of AM decReased slightly, but the the activity of SS went up maRkedly,and the staRch content Rose obviously.
SONG Shu-fang
,
CHEN Jian-jun
,
ZHOU Ji-heng
. Effects of Leaf Population on StaRch Accumulation and Activity of Amylase and SucRose Synthase in Tobacco Leaves[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(1)
: 149
-154
.
DOI: 10.3969/j.issn.1000-7091.2013.01.028
[1] 王怀珠,吕芬,杨焕文. 烤烟淀粉代谢及对烟叶香吃 味的影响[J]. 云南农业大学学报, 2004, 19( 3) : 290 - 294.
[2] 张明方,李志凌. 高等植物中与蔗糖代谢相关的酶
[J]. 植物生理学通讯, 2002, 38( 3) : 289 - 295.
[3] Elling L. Effect of metal ions on sucRose synthase fRom Rice gRains-a study on enzyme inhibition and enzyme topogRaphy
[J]. Glycobiology, 1995,5 ( 2) : 201 - 206.
[4] ZRenneR R,Salanoubat M,WillmitzeR L, et al. Evidence of the cRucial Role of sucRose synthase foR sink stRength using tRansgenic potato plants ( Solanum tubeRosum L.) [J]. Plant JouRnal, 1995,7 : 97 - 107.
[5] D'Aoust M A,Yelle S,Quoc B N. Antisense inhibition of tomato fRuit sucRose synthase decReases fRuit setting and the sucRose unloading capacity of young fRuit[J]. Plant Cell, 1999, 11: 2407 - 2418.
[6] Chengappa S,GuilleRoux M,Phillips W. TRansgenic tomato plants with decReased sucRose synthase aRe unalteRed in staRch and sugaR accumulation in the fRuit[J]. Plant MoleculaR Biology, 1999, 40: 213 - 221.
[7] Whittingham C P,Keys A J,BiRd I F. The enzymology of sucRose synthesis in leaves[J]. Encyclopedia of Plant Physiology, 1979( 6) : 313 - 326.
[8] Akazawa T,Okamoto K. Biosynthesis and metabolism of sucRose[J]. BiochemistRy of Plant, 1980( 3) : 199 - 220.
[9] Yang J,Zhang J,Wang Z, et al. Activities of enzymes involved in sucRose-to-staRch metabolism in Rice gRains subjected to wateR stRess duRing filling[J]. Field CRops ReseaRch, 2003( 2) : 47 - 58.
[10] McCollum T G,HubeR D J,Cantliffe D J. Soluble sugaR accumulation and activity of Related enzymes duRing muskmelon fRuit development[J]. JameR Soc HoRt Science, 1988, 113: 399 - 403.
[11] Wang H L,Lee P D,Chen W L, et al. Osmotic stRessinduced changes of sucRose metabolism in cultuRed sweet potato cells[J]. JouRnal Exp Botany, 2000( 4) : 33 - 37.
[12] Yang Jianchang,Peng Shaobing,Zhang Zujian,et al. GRain and DRy MatteR Yields and PaRtitioning of Assimilates in JaponicaIndica HybRid Rice[J]. CRop Science, 2002( 3) : 17 - 29.
[13] 熊福生,高煜珠,詹勇昌,等. 植物叶片蔗糖、淀粉积 累与其降解酶活性关系研究[J]. 作物学报,1994,1 ( 1) : 52 - 58.
[14] 王怀珠,杨焕文,郭红英,等. 淀粉类酶降解鲜烟叶中 淀粉的研究[J]. 中国烟草科学, 2005( 2) : 37 - 39.
[15] 宫长荣,王能如,汪耀富. 烟叶烘烤原理[M]. 北京: 科学出版社, 1994.
[16] 宫长荣,袁红涛,陈江华. 烘烤过程中环境湿度和烟 叶水分与淀粉代谢动态[J]. 中国农业科学2003,36 ( 2) : 155 - 158.
[17] 宫长荣,袁红涛,陈江华. 烤烟烘烤过程中烟叶淀粉 酶活性变化及色素降解规律的研究[J]. 中国烟草学 报, 2002,8 ( 2) : 16 - 20.
[18] 王怀珠,杨焕文,郭红英. 烘烤过程中温湿度对烤烟 淀粉降解及相关酶活性的影响[J]. 作物学报, 2006, 32( 2) : 313 - 316.
[19] 王怀珠. 杨焕文,郭红英. 烘烤过程中不同成熟度烟 叶淀粉的降解动态[J]. 烟草科技, 2004( 4) : 36 - 39.
[20] 龚顺禹,杨焕文,王怀珠,等. 烟叶中淀粉降解酶活性 与烘烤温湿度的关系研究[J]. 华北农学报, 2005, 20 ( 4) : 17 - 20.
[21] 尹启生,蔡宪杰,王信民,等. 大田中后期烤烟淀粉酶 活性及淀粉含量的变化[J]. 烟草科技,2006( 9) : 55 - 57, 64.
[22] 杨焕文,李佛琳,耿宗泽,等. 烤烟大田生长期淀粉酶 变化及淀粉的积累[J]. 西南农业大学学报: 自然科 学版, 2003, 25( 4) : 321 - 323.
[23] 邓云龙,孔光辉,武锦坤,等. 氮素营养对烤烟叶片淀 粉积累及SPS、淀粉酶活性的影响[J]. 烟草科技, 2001( 11) : 34 - 37.
[24] 刘雷,黄立栋. 变黄阶段温度和空气相对湿度对烟 叶淀粉含量的影响[J]. 河南农业科学, 2007( 6) : 46 - 48, 53.
[25] 李洪勋,王怀珠. 在不同烘烤环境下烟叶淀粉酶和淀 粉磷酸化酶活性的变化规律研究[J]. 安徽农业科 学, 2007, 35( 29) : 9281 - 9282, 9297.
[26] 邹琦. 植物生理学实验指导[M]. 北京: 中国农业 出版社, 1995: 32 - 35.
[27] 阮妙鸿. 钾钙镁营养的相互关系及其对烤烟碳氮代 谢的影响[D]. 福州: 福建农林大学, 2004.
[28] 雷志栋,扬诗秀,许志荣,等. 土壤特性空间变异性初 步研究[J]. 水利学报, 1985( 9) : 10 - 21.
[29] 韩锦峰,史宏志,官春云,等. 不同施氮水平和氮素来 源烟叶碳氮比及其与碳氮代谢的关系[J]. 中国烟草 学报, 1996( 1) : 19 - 25.