According to sequences of PRV and PPV published in GenBank,two pairs of primers were designed,single PCR of PRV and PPV were established,The duplex PCR was developed by optimizing action conditions,This method could detect the template DNA of 10 -10.5/100 μL LTCID50 for PRV,and 10-9.5/100 μL LTCID50 for PPV.Twenty organ samples from different farms of Henan province were respectively detected by duplex PCR,out of which five were PRV positive,eight were PPV positive,three were both PRV and PPV positive,control samples were negative.It suggested that the duplex PCR of PRV and PPV was a rapid,specific and sensitive diagnostic method.
ZHAO Li
,
CUI Bao-an
,
WEN Ying-hui
,
CHEN Hong-ying
. Detection of Porcine PRV and PPV by Duplex Polymerase Chain Reaction[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 206
-209
.
DOI: 10.7668/hbnxb.2009.02.042
[1] 殷震,刘景华.动物病毒学[M].北京:科学出版社,1997.
[2] Kim J,Choi C,Chae C.Pathogenes is of postweaning multisystem icwasting syndrome reproduced byco-infection with Korean solates of pot cine circovirus 2 and porcine parvovirus[J].Journal of Comparative Pathology,2003,128 (1):52-59.
[3] 许立华,王玲,芦银华,等.三种猪繁殖障碍性病毒混合感染的分子生物学调查[J].中国兽医科技,2004,34(7):40-43.
[4] 甘孟侯.当前我国猪传染病的发生特点及防治对策[J].中国兽医志,2005,41(5):64-66.
[5] 张焕容,肖驰,曹三杰,等.猪伪狂犬病、猪细小病毒病及猪流行性乙型脑炎多重PCR诊断方法的建立[J].黑龙江畜牧兽医,2005,7:7-9.
[6] 刘建柱,崔玉东,李鹏,等.多重PCR检测猪瘟病毒、猪细小病毒、猪伪狂犬病病毒[J].中国兽医学报,2003,23(6):535-537.
[7] Choi C,Chae C.Distribution of porcine parvovirus in porcine circovirus 2-infected pigs with postweaning multisystem icwasting syndrome as shown by in-situ hybridization[J].Journal of Comparative Pathology,2000,123 (4):302-305.
[8] 黄伟坚,姜焱,陈德胜,等.伪狂犬病毒上海株糖蛋白G(gG)基因的克隆及序列分析[J].南京农业大学学报,2003,26(1):107-110.
[9] 赵丽,崔保安,方忠意,等.PCR检测猪伪狂犬病病毒方法的研究[J].中国预防兽医学报,2007,29(2):142-146.
[10] 萨姆布鲁克,拉塞尔D W著,黄培堂等译,分子克隆实验指南[M].第3版.北京:科学出版社,2002.
[11] 周海范,夏平安,崔保安,等.猪繁殖与呼吸综合征、猪圆环病复合PCR诊断方法的建立及应用[J].河南农业科学,2007(01):67-70.
[12] 赵绪永,张改平,朱礼倩,等.鸡胚成纤维细胞CDNATT噬菌体表达文为的构成和鉴定[J].河南农业科学,2008(7):102-105.
