According to sequences of PRV and PPV published in GenBank,two pairs of primers were designed,single PCR of PRV and PPV were established,The duplex PCR was developed by optimizing action conditions,This method could detect the template DNA of 10 -10.5/100 μL LTCID50 for PRV,and 10-9.5/100 μL LTCID50 for PPV.Twenty organ samples from different farms of Henan province were respectively detected by duplex PCR,out of which five were PRV positive,eight were PPV positive,three were both PRV and PPV positive,control samples were negative.It suggested that the duplex PCR of PRV and PPV was a rapid,specific and sensitive diagnostic method.
ZHAO Li
,
CUI Bao-an
,
WEN Ying-hui
,
CHEN Hong-ying
. Detection of Porcine PRV and PPV by Duplex Polymerase Chain Reaction[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 206
-209
.
DOI: 10.7668/hbnxb.2009.02.042
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