In order to privide some knowledge for transgenic plant feed, primeres were designed on transgenic soy2 bean meal within CaMV35 S promoter gene and NOS terminator, and effect of heat treatment, autoclave, and other process2 ing technics on degradation of CaMV35 S and NOS in soybean meal was investigated by polymerase chain reaction detec2 tion system in this experiment.The results showed that heat treatment had no effect on degradation of both CaMV35S and NOS.Autoclave treatment also did not affect degradation of CaMV35S,and 246,165,and 101 bp fragments of CaMV35S were all detectable,but resulted in degradation of NOS,and 125 bp fragment was detectable and 217 bp fragment was undetectable.And the same result was obtained also in feeds those processed fromsoybean meal.The promoter gene was relatively stable and terminator gene was relatively easily destroyed.
LIANG Shan
,
LIANG Ning
,
JIANG Ji-zhi1
,
HU Dong-sheng
. Degradation of Non-target Genes During Feed Processes of Genetically Modified Soybean Meal[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 201
-205
.
DOI: 10.7668/hbnxb.2009.02.041
[1] Marc V,Hans P,Franco G,et al.Real-time quantitative PCR detection of genetically modified maximizermaize and roundup ready soybean in some rep resentafive foods[J].Agric Food Chem,1999,47:5261-5266.
[2] Kohli A,Griffiths S,Palacins N,et al.Molecular characteriza tion of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV35S promoter and confirms the predominance of microhomology mediated recombination[J].Plant,1999,17:591-601.
[3] Cummins J,Ho M W,Ryan A.Hazardous CaMV promoter[J].Nature Biotechnol,2000,18:363.
[4] 邵碧英,江树.大豆及其制品中转基因成分的二重PCR检测[J].中国食品学报,2006,6(3):115-120.
[5] 陶震,杨胜利,龚毅.利用MPCR方法快速检测植物转基因背景[J].生物技术通报,2000(6):37-41.
[6] 金红,程奕,赵昕,等.大豆色拉油中转基因成分检测技术研究[J].华北农学报,2004,19(1):24-27.
[7] 蒋原,祝长青,林宏,等.利用PCR技术检测转基因大豆Roundup Ready的研究[J].检验检疫科学,2001,11(4):11-13.
[8] Rottm E,Lawrence T S,Wall E M,et al.Detection and quantification of roundup ready soy in foods by conventional and rea12time polymerase chain reaction[J].J Agric Food Chem,2004,52 (16):5223-5232.
[9] 陈颖,王嫒,葛毅强,等.转基因大豆调控元件在食品加工过程中降解变化的研究[J].中国食品卫生杂志,2005,17(2):135-139.
[10] Miraglia M,Berdal K Q Brera C,et al.Detectinn and traceability of genetically modified organisms in the food production chain[J].Food and Chemical Toxicology,2004,42:1157-1180.
[11] Takeshi Matsuoka,Hideo Kurihara,Ken Takubo,et al.Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays)[J].J Agric Food Chem,2002,50:2100-2109.
[12] Nobuaki Shirai,Keiko Momma,Sachiko Ozawa,et al.Safety assessment of genetically engineered food:detection and monitoring of glyphosate-toletant soybeans[J].Biosci Biotechnol Biocbem,1998,62(7):1461-1464.
[13] 赵文军,陈红运,黄文胜,等.PCR-ELISA在转基因产品检测中的应用[J].植物检疫,2001,15(5):297-299.
[14] Catherine F Terry,Della J Shanahan,Lydia D Ballam,et al.Real-time detection of genetically modified soya using lightcycle and ABI 7700 plant forms with TaqMan,Scorpion,and SYBR Green I Chemistrins[J].Journal of AOAC international,2002,85(4):938-944.
[15] 郑文杰,刘伟.转基因大豆加工产品的定性PCR检测[J].农业生物技术学报,2003,11(5):467-471.
[16] Torsten Bauer,Philipp Weller,Walter P,et al.The effect of processing parameters on DNA degradation in food[J].Eur Food Res Teehnol,2003,217:338-343.
