In this paper, a pot experiment was conducted to study the effects of different nitrogen application on nodule growth,nodulation and nitrogen fixation in soybean( Glycnie max L.).The results showed that nitrogen application affected nodule forming, growth and nitrogen fixation capacity significantly, nodule dryweight and nodule number showed atrend of increasing first and then declining,while nitrogenase activity and leghemoglobin concentration showed the trend of continuosly declining.Proper N application improved nodule growth,however N deficiency or excessive N use restrained nodule growth.There was an order of N100> N200> N50> N25> N0 for nodule dry weight and nodule number.At different growth stages,nodule dryweight showed a trend of increasing first and then declining from seedling stage to podding stage,and for nodule number was flowering stage> seedling stage.N application significantly decreased nitrogenase activity and leghemoglobin concentration with N rates increasing,showed an order of N0> N25> N50> N100> N200, suggested that N application decreased the efficiency of nitrogen fixation.The difference analysis showed that there was a signifi2 cant difference between N application(N25, N50, N100 and N200) and no N(N0) treatment.Therefore,from the view of the balance between N fertilization and nitrogen fixation,proper N application not only makes the best of nitrogen fixation function which economizing N fertilizer application but also obtaining higher output of soybean.
WANG Shu-qi
,
HAN Xiao-zeng
,
QIAO Yun-fa
,
YAN Jun
,
LI Xiao-hui
. Nodule Growth, Nodulation and Nitrogen Fixation in Soybean(Glycine max L.)as Affected by Nitrogen Application[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 176
-179
.
DOI: 10.7668/hbnxb.2009.02.036
[1] Ohwaki Y,Sugahara P.Active extrusion of protons and exudation of carbexylic acids in response to iron deficiency by roots of chickpea(Cicer arietinum L.)[J].Plant Soil,1997,189:49-55.
[2] 桑原真人.タィスの多收条件と室素代谢[J].农业よび园艺,1986,61(5):590-598.
[3] 藤田耕之辅.タィズによける室素の固定、吸收、转流に对する化合室素の影响[J].土壤肥料学杂志,1982,53(1):30-34.
[4] 宋海星,王萍,申斯东,等.大豆共生固氮与叶片全氮含量之间关系的研究[J].吉林农业科学,2000,25(6):911.
[5] Streeter J.Inhibition of legume nodule formation and N2 fixation by nitrate[J].CRC Crit Rev Plant Sci,1988,7:1-23.
[6] Kennedy I R.Primary products of symbiotic nitrogen fixation I.Short-term exposures of serradella nodules to 15N2[J].Biochim Biophys Acta,1966,130:285-294.
[7] 藤田耕之辅.タィズおよびレンクの生育をリびに室素の固定に对する化合态室素ゃ碳酸力スの影响[J].土壤肥料学杂志,1986,57(1):3-12.
[8] Wall L G,Hellstcn A,Huss-Dancll K.Nitrogen,phosphorus,and the ratio between them affect nodulation in Alnus incana and Trifolium pretense[J].Symbiosis,2000,29:91-105.
[9] Tang C,Hinsingcr P J,Drevon J,et al.Phosphorus deficiency impairs early nodule functioning and enhances proton release in Roots of Medicago truncatula L.[J].Annals of Botany,2001,88:131-138.
[10] 左元梅,刘永秀,张福锁.与玉米混作改善花生铁营养对其根瘤形态结构及豆血红蛋白含量的影响[J].植物生理与分子生物学学报,2003,29(1):33-38.
[11] 甘银波,陈静.大豆不同生长阶段施用氮肥对生长、结瘤及产量的影响[J].大豆科学,1998,17(4):45-49.
[12] Eagiesham A R J.Aerial sterna nodules on Aeschyrmncne spp[J].Plant Sci.,1983,29:265-273.
[13] Mukhtar N O,Badreidin A M.NPK fertil ization of groundnuts(Arechis hypogara L.) under irrigated environment[J].Sudan J Agri Res,1998,1 (1):21-26.
[14] 解惠光.日本大豆氮素营养与施肥研究最新进展[J].大豆科学,1990,9(2):163-167.
[15] 张学江.农业生态系统中的共生固氮研究[J].土壤学进展,1990,18(5):1-8,14.
[16] 丁洪,郭庆元.氮肥对大豆不同类型品种结瘤固氮影响的差异性研究[J].大豆科学,1994,13(3):274-278.
