Eight types of blood proteins in serum samples fromMongolian horse,Sanhe horse and Thoroughbred horse were detected on polymorphism by vertical plate discontinuous polyacrylamide gel electrophoresis (PAGE).Genotype frequencies,gene frequency and genetic heterozygosity of these sites were calculated,and the relationship and the genetic distance between the groups were discussed through cluster analysis.The results indicated that eight blood protein (enzyme) sites were of polymorphism.The result showed the average heterozygosity(H) of these eight blood protein (enzyme) sites in Mongolian horse,Sanhe horse and Thoroughbred horse were 0.3782,0.3956,0.2571,respectively.The cluster analysis suggested that the relationship between Mongolian horse and Sanhe horse was closer,however,the relationship between these two types of horses and Thoroughbred horse were relatively farther.
YANG Hong
,
MENG Ke
,
WANG Ye-hui
,
Dugarjaviin Manglai
. Genetic Polymorphism and Cluster Analysis on Blood Protein (Enzyme)of Different Breeds of Horses[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(2)
: 103
-106
.
DOI: 10.7668/hbnxb.2009.02.022
[1] Ashton G C.Serum protein variations in horses[J].Nature,1958,182:1029-1030.
[2] Kaminski M,Gajos E.Comparative examination of carboxylic esterases in sera of horse,donkey and their hybrids[J].Nature,1964,201:716-718.
[3] 王振山.采用血液遗传标记对马亲仔关系案例的检测与分析[J].黑龙江畜牧兽医,2001(11):4-6.
[4] 张才骏,王勇.玉树马血清酯酶的多态性[J].青海畜牧兽医杂志,2001(1):1-2.
[5] Sandberg K.Guidelines for the interpretation of blood typing tests in horses[M].Brisbane,1989,1-8.
[6] 谢成侠.中国养马史[M].北京:农业出版社,1991:193-194.
[7] 王振山,郭永新,李建成.三河马血液遗传标记的研究[J].内蒙古畜牧科学,2001(2):1-3.
[8] 赵天佐.纯血马是世界名贵马种[C] //现代马业选集.北京:中国农业科学技术出版社,2007:269-272.
[9] Diaz S,Dulout F N,Peral-Gareia P.Greater genetic variability in argentine creole than in thoroughbred horses based on serum protein polymorphisms[J].Genetics and Molecular Research,2002,1 (3):261-265.
[10] 李金莲,石有斐,布仁其其格,等.三大不同品种马mtDNA Cyt 6基因PCR-RFLP分析[J].遗传,2006,28(8):933-938.
[11] 孟青龙.马MHC-Ⅱ类分子遗传多样性的研究[D].呼和浩特:内蒙古农业大学,2005.
[12] 李金莲.中国蒙古马遗传多样性和分子系统进化研究[D].呼和浩特:内蒙古农业大学,2006.
