杜梨<i>PbKRP2</i>序列特征与表达特性分析

doi:10.7668/hbnxb.20196258

摘要/Abstract

摘要：

KRP基因家族作为细胞周期调控的关键因子,通过抑制周期蛋白依赖性激酶(CDK)活性调节细胞分裂与核内复制进程,并参与植物非生物胁迫响应。为探索杜梨PbKRP2的结构特征和对环境胁迫的响应特性,采用逆转录PCR技术进行PbKRP2的基因克隆,并结合生物信息学方法对其序列特征、蛋白互作及亲缘关系等进行分析,通过荧光定量PCR技术检测杜梨PbKRP2的组织表达特性和在不同逆境胁迫下的表达模式。结果表明,该基因全长585 bp,包含4个外显子和3个内含子,编码194个氨基酸,含有25个潜在磷酸化位点和1个O-糖基化修饰位点,且在该蛋白C端具有周期蛋白依赖性激酶抑制因子家族特有的CDI结构域;进化树分析显示,杜梨PbKRP2与裸花东京樱花PyKRP2、桃PpKRP2和苹果MdKRP2的亲缘关系较为接近;蛋白质互作网络筛选发现,PbKRP2与10个蛋白存在潜在互作关系,互作蛋白静态结构分析发现,PbKRP2与其靶蛋白PbCDKB1;1和PbCYCD7;1形成空间互作构型;该基因启动子区存在多个响应环境因子与激素信号的顺式调控元件;实时荧光定量PCR结果表明,PbKRP2基因在茎和叶中表达量显著高于根、花瓣和果实,且该基因的转录水平在低温胁迫和ABA处理时持续上调,脱水和盐胁迫下显著下调。综上所述,通过解析PbKRP2的序列特性及其对非生物胁迫的差异化响应模式,为梨抗逆育种及果树遗传改良提供了候选基因。

关键词: 杜梨, PbKRP2基因, 基因克隆, 非生物胁迫, 表达分析

Abstract:

The Kip-related protein(KRP)gene family acts as a key regulator of the cell cycle,modulating cell division and endoreduplication by inhibiting cyclin-dependent kinase(CDK)activity,and participates in plant abiotic stress responses.To investigate the structural characteristics and environmental stress response profile of PbKRP2 in Pyrus betulifolia,the gene was cloned using reverse transcription PCR(RT-PCR).Bioinformatic analysis was conducted to elucidate its sequence features,predicted protein interactions,and phylogenetic relationships.Quantitative Real-time PCR(qRT-PCR)was employed to assess the tissue-specific expression characteristics of PbKRP2 and its expression patterns under various stress conditions.Results showed that PbKRP2 was 585 bp in length,contained 4 exons and 3 introns,and encoded a 194-amino acid protein.The predicted protein had 25 potential phosphorylation sites,a O-glycosylation site,and a conserved C-terminal cyclin-dependent kinase inhibitor(CDI)domain.Phylogenetic analysis indicated that PbKRP2 was closely related to PyKRP2 in Prunus yedoensis var.nudiflora,PpKRP2 in Prunus persica,and MdKRP2 in Malus domestica.Protein interaction network screening identified potential interactions between PbKRP2 and 10 proteins.Analysis of the static structure of potential interacting proteins suggested that PbKRP2 formed spatial interaction conformations with its target proteins PbCDKB1;1 and PbCYCD7;1,respectively.The promoter region of PbKRP2 contained multiple cis-acting regulatory elements responsive to environmental factors and hormonal signals.qRT-PCR analysis demonstrated that PbKRP2 transcript levels were significantly higher in stems and leaves compared to roots,petals,and fruits.Furthermore,PbKRP2 transcription levels were consistently upregulated under low-temperature stress and abscisic acid(ABA)treatment,but significantly downregulated under dehydration and salt stress.In summary,these findings provide a candidate gene for stress-resistant breeding of pear and genetic improvement of fruit trees by elucidating the sequence characteristics of PbKRP2 and its differential response patterns to abiotic stresses.

Key words: Pyrus betulaefolia, PbKRP2 gene, Gene cloning, Abiotic stress, Expression analysis

中图分类号:

靳丛, 徐连理, 刘琦, 张晓小, 张宇双, 徐源, 陈钰祺, 任雅楠, 王纪忠. 杜梨PbKRP2序列特征与表达特性分析[J]. 华北农学报, 2026, 41(2): 36-46. doi: 10.7668/hbnxb.20196258.

JIN Cong, XU Lianli, LIU Qi, ZHANG Xiaoxiao, ZHANG Yushuang, XU Yuan, CHEN Yuqi, REN Yanan, WANG Jizhong. Analysis of Sequence Characteristics and Expression Patterns of PbKRP2 in Pyrus betulaefolia[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(2): 36-46. doi: 10.7668/hbnxb.20196258.

http://www.hbnxb.net/CN/Y2026/V41/I2/36

图/表 12

图1 PbKRP2基因扩增电泳 M.DL2000 DNA Marker;1—2.PbKRP2扩增产物。

Fig.1 Electrophoretogram of amplification product of the PbKRP2 gene M.DL2000 DNA Marker;1—2.PbKRP2 amplification products.

图2 PbKRP2核苷酸序列及其编码的氨基酸序列

Fig.2 Nucleotide sequence of PbKRP2 and its encoded amino acid sequence

图3 PbKRP2基因结构(A)及保守结构域(B)预测

Fig.3 Prediction of the PbKRP2 gene structure(A)and conserved domains(B)

图4 PbKRP2蛋白的三级结构预测紫色高亮区域表示保守的CDI结构域。

Fig.4 Tertiary structure prediction of PbKRP2 protein The purple highlighted regions represent conserved CDI structural domains.

图5 PbKRP2蛋白磷酸化和糖基化修饰位点预测

Fig.5 Prediction of phosphorylation and glycosylation site of PbKRP2 protein

图6 PbKRP2互作蛋白网络预测

Fig.6 Predicted protein-protein interaction network of PbKRP2

图7 PbKRP2与其靶蛋白互作的静态复合物结构预测 A.PbKRP2-PbCDKB1;1复合物;B.PbKRP2-PbCYCD7;1复合物。

Fig.7 Structural prediction of the static complex formed by PbKRP2 interaction with its target proteins A.The PbKRP2-PbCDKB1;1 complex;B.The PbKRP2-PbCYCD7;1 complex.

图8 PbKRP2与同源蛋白的多序列比对实线区域为核定位信号;星号标注为酸性-疏水簇。

Fig.8 Multiple sequence alignment of PbKRP2 with other homologous proteins Areas marked with solid lines indicate the nuclear localization signal;positions marked with asterisks(*)denote the acidic-hydrophobic cluster.

图9 PbKRP2与其他同源蛋白的系统进化(A)及保守基序分析(B)

Fig.9 Phylogenetic analysis of PbKRP2 and other homologous proteins(A)and conserved motif analysis(B)

图10 PbKRP2基因顺式作用元件分析

Fig.10 Cis-acting element analysis of the PbKRP2 gene

图11 PbKRP2在不同组织的表达水平图柱上不同小写字母表示各处理之间存在差异显著(P<0.05)。图12同。

Fig.11 Expression patterns of the PbKRP2 gene in different tissues Different lowercase letters above bars indicate significant differences among treatments(P<0.05).The same as Fig.12.

图12 PbKRP2在不同非生物胁迫下的表达特性 A.4 ℃低温处理;B.脱水处理;C.200 mmol/L NaCl处理;D.100 μmol/L ABA处理。

Fig.12 Expression profiling of PbKRP2 in response to various abiotic stresses A.4 ℃ treatment;B.Dehydration treatment;C.200 mmol/L NaCl treatment;D.100 μmol/L ABA treatment.

参考文献 39

[1]	Harashima H, Dissmeyer N, Schnittger A. Cell cycle control across the eukaryotic Kingdom[J]. Trends in Cell Biology, 2013, 23(7):345-356.doi:10.1016/j.tcb.2013.03.002. pmid: 23566594
[2]	Inzé D, De Veylder L. Cell cycle regulation in plant development[J]. Annual Review of Genetics, 2006, 40:77-105.doi:10.1146/annurev.genet.40.110405.090431. pmid: 17094738
[3]	Sherr C J, Roberts J M. CDK inhibitors:positive and negative regulators of G1-phase progression[J]. Genes & Development, 1999, 13(12):1501-1512.doi:10.1101/gad.13.12.1501. URL
[4]	Wang H, Fowke L C, Crosby W L. A plant cyclin-dependent kinase inhibitor gene[J]. Nature, 1997, 386(6624):451-452.doi:10.1038/386451a0.
[5]	De Veylder L, Beeckman T, Beemster G T S, Krols L, Terras F, Landrieu I, Van Der Schueren E, Maes S, Naudts M, Inzé D. Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis[J]. The Plant Cell, 2001, 13(7):1653-1668.doi:10.1105/tpc.010087. URL
[6]	Wang H, Qi Q G, Schorr P, Cutler A J, Crosby W L, Fowke L C. ICK1,a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3,and its expression is induced by abscisic acid[J]. The Plant Journal, 1998, 15(4):501-510.doi:10.1046/j.1365-313x.1998.00231.x. URL
[7]	Wang H, Zhou Y M, Fowke L C. The emerging importance of cyclin-dependent kinase inhibitors in the regulation of the plant cell cycle and related processes[J]. Canadian Journal of Botany, 2006, 84(4):640-650.doi:10.1139/b06-043. URL
[8]	Coelho C M, Dante R A, Sabelli P A, Sun Y J, Dilkes B P, Gordon-Kamm W J, Larkins B A. Cyclin-dependent kinase inhibitors in maize endosperm and their potential role in endoreduplication[J]. Plant Physiology, 2005, 138(4):2323-2336.doi:10.1104/pp.105.063917. pmid: 16055680
[9]	Barrôco R M, Peres A, Droual A M, De Veylder L, Nguyen L S L, De Wolf J, Mironov V, Peerbolte R, Beemster G T S, Inzé D, Broekaert W F, Frankard V. The cyclin-dependent kinase inhibitor Orysa;KRP1 plays an important role in seed development of rice[J]. Plant Physiology, 2006, 142(3):1053-1064.doi:10.1104/pp.106.087056. pmid: 17012406
[10]	Jasinski S, Leite C S, Domenichini S, Stevens R, Raynaud C, Perennes C, Bergounioux C, Glab N. NtKIS2,a novel tobacco cyclin-dependent kinase inhibitor is differentially expressed during the cell cycle and plant development[J]. Plant Physiology and Biochemistry, 2003, 41(6/7):667-676.doi:10.1016/S0981-9428(03)00082-2. URL
[11]	Nafati M, Frangne N, Hernould M, Chevalier C, G vaudant F. Functional characterization of the tomato cyclin-dependent kinase inhibitor SlKRP1 domains involved in protein-protein interactions[J]. New Phytologist, 2010, 188(1):136-149.doi:10.1111/j.1469-8137.2010.03364.x.
[12]	Pettkó-Szandtner A, Mészáros T, Horváth G V, Bakó L, Csordás-Tóth E, Blastyák A, Zhiponova M, Miskolczi P, Dudits D. Activation of an alfalfa cyclin-dependent kinase inhibitor by calmodulin-like domain protein kinase[J]. The Plant Journal, 2006, 46(1):111-123.doi:10.1111/j.1365-313x.2006.02677.x. URL
[13]	Torres Acosta J A, Fowke L C, Wang H. Analyses of phylogeny,evolution,conserved sequences and genome-wide expression of the ICK/KRP family of plant CDK inhibitors[J]. Annals of Botany, 2011, 107(7):1141-1157.doi:10.1093/aob/mcr034. URL
[14]	Ormenese S, de Almeida Engler J, De Groodt R, De Veylder L, Inzé D, Jacqmard A. Analysis of the spatial expression pattern of seven Kip related proteins(KRPs)in the shoot apex of Arabidopsis thaliana[J]. Annals of Botany, 2004, 93(5):575-580.doi:10.1093/aob/mch077. pmid: 15037450
[15]	Jun S E, Okushima Y, Nam J, Umeda M, Kim G T. Kip-related protein 3 is required for control of endoreduplication in the shoot apical meristem and leaves of Arabidopsis[J]. Molecules and Cells, 2013, 35(1):47-53.doi:10.1007/s10059-013-2270-4. URL
[16]	Jégu T, Latrasse D, Delarue M, Mazubert C, Bourge M, Hudik E, Blanchet S, Soler M N, Charon C, De Veylder L, Raynaud C, Bergounioux C, Benhamed M. Multiple functions of Kip-related protein5 connect endoreduplication and cell elongation[J]. Plant Physiology, 2013, 161(4):1694-1705.doi:10.1104/pp.112.212357. pmid: 23426196
[17]	Guan C F, Ji J, Guan W Z, Li X Z, Jin C, Li J, Wang Y R, Wang G. LcKRP,a cyclin-dependent kinase inhibitor from Lycium chinense,is involved in an ABA-dependent drought stress-signaling pathway[J]. Plant and Soil, 2014, 382(1):43-59.doi:10.1007/s11104-014-2134-5. URL
[18]	Liu G Z, Guan Z F, Ma M X, Wang H Y, Liu X F, Song S Y, Dai N Y, Ma F F, Bao Z L. Genome-wide identification and molecular characterization of SlKRP family members in tomato and their expression profiles in response to abiotic stress[J]. Vegetable Research, 2023, 3(1):27.doi:10.48130/vr-2023-0027.
[19]	Shen L, Yang S X, Xia X, Nie W F, Yang X. Genome-wide identification of Kip-related protein(KRP)gene family members in eggplant and the function of SmKRP3 under salt stress[J]. Vegetable Research, 2024, 4(1):e013.doi:10.48130/vegres-0024-0012.
[20]	Guo B H, Chen L, Dong L, Yang C H, Zhang J H, Geng X Y, Zhou L J, Song L. Characterization of the soybean KRP gene family reveals a key role for GmKRP2a in root development[J]. Frontiers in Plant Science, 2023, 14:1096467.doi:10.3389/fpls.2023.1096467. URL
[21]	张绍铃, 谢智华. 我国梨产业发展现状、趋势、存在问题与对策建议[J]. 果树学报, 2019, 36(8):1067-1072.doi:10.13925/j.cnki.gsxb.20190033.
	Zhang S L, Xie Z H. Current status,trends,main problems and the suggestions on development of pear industry in China[J]. Journal of Fruit Science, 2019, 36(8):1067-1072.
[22]	董星光, 曹玉芬, 王昆, 田路明, 张莹, 齐丹. 中国3个主要梨砧木资源木质部导管分子结构及分布比较[J]. 植物学报, 2015, 50(2):227-233.doi:10.3724/SP.J.1259.2015.00227.
	Dong X G, Cao Y F, Wang K, Tian L M, Zhang Y, Qi D. Comparison of the characters and distribution of vessel elements in xylem among three main pear rootstocks in China[J]. Chinese Bulletin of Botany, 2015, 50(2):227-233. doi: 10.3724/SP.J.1259.2015.00227 URL
[23]	Chen C J, Wu Y, Li J W, Wang X, Zeng Z H, Xu J, Liu Y L, Feng J T, Chen H, He Y H, Xia R. TBtools-Ⅱ:a “one for all,all for one” bioinformatics platform for biological big-data mining[J]. Molecular Plant, 2023, 16(11):1733-1742.doi:10.1016/j.molp.2023.09.010. URL
[24]	Szklarczyk D, Kirsch R, Koutrouli M, Nastou K, Mehryary F, Hachilif R, Gable A L, Fang T, Doncheva N T, Pyysalo S, Bork P, Jensen L J, von Mering C. The STRING database in 2023:protein protein association networks and functional enrichment analyses for any sequenced genome of interest[J]. Nucleic Acids Research, 2023, 51(D1):D638-D646.doi:10.1093/nar/gkac1000.
[25]	Abramson J, Adler J, Dunger J, Evans R, Green T, Pritzel A, Ronneberger O, Willmore L, Ballard A J, Bambrick J, Bodenstein S W, Evans D A, Hung C C, O'Neill M, Reiman D, Tunyasuvunakool K, Wu Z, Žemgulytè A, Arvaniti E, Beattie C, Bertolli O, Bridgland A, Cherepanov A, Congreve M, Cowen-Rivers A I, Cowie A, Figurnov M, Fuchs F B, Gladman H, Jain R, Khan Y A, Low C M R, Perlin K, Potapenko A, Savy P, Singh S, Stecula A, Thillaisundaram A, Tong C, Yakneen S, Zhong E D, Zielinski M, Žídek A, Bapst V, Kohli P, Jaderberg M, Hassabis D, Jumper J M. Accurate structure prediction of biomolecular interactions with AlphaFold 3[J]. Nature, 2024, 630(8016):493-500.doi:10.1038/s41586-024-07487-w.
[26]	Seeliger D, de Groot B L. Ligand docking and binding site analysis with PyMOL and Autodock/Vina[J]. Journal of Computer-Aided Molecular Design, 2010, 24(5):417-422.doi:10.1007/s10822-010-9352-6. pmid: 20401516
[27]	Tamura K, Stecher G, Kumar S. MEGA11:molecular evolutionary genetics analysis version 11[J]. Molecular Biology and Evolution, 2021, 38(7):3022-3027.doi:10.1093/molbev/msab120. URL
[28]	冯清香, 朱哈修, 杜洋, 徐艳茹, 王晨晨, 刘雪, 李大勇, 周军, 张彬. 菊苣ANS基因的克隆及表达分析[J]. 华北农学报, 2025, 40(3):51-59.doi:10.7668/hbnxb.20195328.
	Feng Q X, Zhu H X, Du Y, Xu Y R, Wang C C, Liu X, Li D Y, Zhou J, Zhang B. Cloning and expression analysis of ANS gene in Cichorium intybus[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3):51-59.
[29]	桑莹莹, 李珊珊, 鲍薇, 徐东, 张雪, 赵艳. 大豆P34蛋白基因启动子克隆及其调控活性分析[J]. 华北农学报, 2025, 40(1):22-28.doi:10.7668/hbnxb.20195348.
	Sang Y Y, Li S S, Bao W, Xu D, Zhang X, Zhao Y. Cloning and regulatory activity analysis of soybean P34 protein gene promoter[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1):22-28. doi: 10.7668/hbnxb.20195348
[30]	Yanai I, Benjamin H, Shmoish M, Chalifa-Caspi V, Shklar M, Ophir R, Bar-Even A, Horn-Saban S, Safran M, Domany E, Lancet D, Shmueli O. Genome-wide midrange transcription profiles reveal expression level relationships in human tissue specification[J]. Bioinformatics, 2005, 21(5):650-659.doi:10.1093/bioinformatics/bti042. pmid: 15388519
[31]	Lim S, Kaldis P. Cdks,cyclins and CKIs:roles beyond cell cycle regulation[J]. Development, 2013, 140(15):3079-3093.doi:10.1242/dev.091744. URL
[32]	Nakai T, Kato K, Shinmyo A, Sekine M. Arabidopsis KRPs have distinct inhibitory activity toward cyclin D2-associated kinases,including plant-specific B-type cyclin-dependent kinase[J]. FEBS Letters, 2006, 580(1):336-340.doi:10.1016/j.febslet.2005.12.018. URL
[33]	Zhou Y M, Wang H, Gilmer S, Whitwill S, Fowke L C. Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth,cell size and ploidy in Arabidopsis thaliana[J]. Planta, 2003, 216(4):604-613.doi:10.1007/s00425-002-0935-x.
[34]	Zhou Y M, Li G Y, Brandizzi F, Fowke L C, Wang H. The plant cyclin-dependent kinase inhibitor ICK1 has distinct functional domains for in vivo kinase inhibition,protein instability and nuclear localization[J]. The Plant Journal, 2003, 35(4):476-489.doi:10.1046/j.1365-313x.2003.01821.x. URL
[35]	Peres A, Churchman M L, Hariharan S, Himanen K, Verkest A, Vandepoele K, Magyar Z, Hatzfeld Y, Van Der Schueren E, Beemster G T S, Frankard V, Larkin J C, Inzé D, De Veylder L. Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses[J]. Journal of Biological Chemistry, 2007, 282(35):25588-25596.doi:10.1074/jbc.M703326200. pmid: 17599908
[36]	Zhou Y M, Niu H S, Brandizzi F, Fowke L C, Wang H. Molecular control of nuclear and subnuclear targeting of the plant CDK inhibitor ICK1 and ICK1-mediated nuclear transport of CDKA[J]. Plant Molecular Biology, 2006, 62(1):261-278.doi:10.1007/s11103-006-9019-9. URL
[37]	Wen B, Nieuwland J, Murray J A H. The Arabidopsis CDK inhibitor ICK3/KRP5 is rate limiting for primary root growth and promotes growth through cell elongation and endoreduplication[J]. Journal of Experimental Botany, 2013, 64(4):1135-1144.doi:10.1093/jxb/ert009. pmid: 23440171
[38]	Cheng Y, Liu H, Cao L, Wang S, Li Y P, Zhang Y Y, Jiang W, Zhou Y M, Wang H. Down-regulation of multiple CDK inhibitor ICK/KRP genes promotes cell proliferation,callus induction and plant regeneration in Arabidopsis[J]. Frontiers in Plant Science, 2015, 6:825.doi:10.3389/fpls.2015.00825. pmid: 26528298
[39]	Lui H, Wang H, Delong C, Fowke L C, Crosby W L, Fobert P R. The Arabidopsis Cdc2a-interacting protein ICK2 is structurally related to ICK1 and is a potent inhibitor of cyclin-dependent kinase activity in vitro[J]. The Plant Journal, 2000, 21(4):379-385.doi:10.1046/j.1365-313x.2000.00688.x. URL

选择文件类型/文献管理软件名称

选择包含的内容

杜梨PbKRP2序列特征与表达特性分析

Analysis of Sequence Characteristics and Expression Patterns of PbKRP2 in Pyrus betulaefolia

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 12

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李若琳, 刘文泽, 常佳迎, 张娜, 杨文香. 小麦叶锈菌效应因子Pt6647 ORF克隆及其表达分析[J]. 华北农学报, 2026, 41(2): 187-193.
[2]	邓满, 高英, 杨柏明, 张卫华, 蔡泽宇, 周歆茗, 吴颖. *西瓜脱落酸受体蛋白基因ClPYL8的克隆、亚细胞定位及表达分析*[J]. 华北农学报, 2026, 41(2): 21-27.
[3]	苏成丽, 牛熙, 黄世会, 李升, 冉雪琴, 王嘉福. 沙福芽孢杆菌多铜氧化酶CotA蛋白锰氧化活性研究[J]. 华北农学报, 2026, 41(1): 230-238.
[4]	吴嘉萁, 许玉洁, 陈洋洋, 任旭琴, 周瑾, 熊爱生, 王广龙. *大蒜水通道蛋白基因AsPIP1;1和AsTIP2;1的克隆、鉴定及其对盐胁迫的响应*[J]. 华北农学报, 2026, 41(1): 64-70.
[5]	刘毅程, 洛桑顿珠, 卓玛次仁, 尼玛加措, 平措占堆, 马晓明, 梁春年, 李少斌. *西藏桑桑牦牛RAN基因克隆、生物信息学及组织表达分析*[J]. 华北农学报, 2026, 41(1): 212-221.
[6]	吉夏洁, 雷雅坤, 刘宁, 孙丽敏, 胡景辉. 小麦非生物胁迫响应机制研究进展及抗逆育种展望[J]. 华北农学报, 2025, 40(S1): 40-53.
[7]	何文斌, 平措占堆, 张强, 尼玛加措, 王洪壮, 余道宁, 张梦帆, 王佟, 冯芬, 喇永富, 马晓明, 洛桑顿珠, 梁春年. *牦牛RPL8基因克隆、生物信息学及组织表达分析*[J]. 华北农学报, 2025, 40(6): 186-193.
[8]	邓婧瑛, 于沁冉, 王佟, 李宁, 贾建磊, 包鹏甲, 阎萍. *帕米尔牦牛PPP1R11基因克隆、生物信息学及组织表达分析*[J]. 华北农学报, 2025, 40(6): 194-201.
[9]	李静, 庞博, 张茹, 扎尔加玛丽·阿不都别克, 王正瑞, 史顺宇, 范珍珍, 马晶晶, 陈佳林, 宋武, 李生梅, 高文伟. *棉花GhCRK26基因克隆、亚细胞定位及表达分析*[J]. 华北农学报, 2025, 40(6): 62-70.
[10]	于佳, 郭慧琴, 李宇霞, 雷慧, 任卫波. *扁蓿豆MrAGL8基因的克隆、亚细胞定位及表达分析*[J]. 华北农学报, 2025, 40(5): 55-61.
[11]	卢振华, 梁晨, 张丽, 佟可心, 陈小强, 李明, 丁博, 邱丽娜, 谢晓东, 王俊斌. *小麦TaWRKY70的克隆、亚细胞定位与表达分析*[J]. 华北农学报, 2025, 40(5): 12-19.
[12]	杜冲, 贺付蒙, 隋嘉, 赵潇璨, 车云竹, 张增利, 刘丹, 王雪, 李凤兰. *马铃薯扩展蛋白基因StEXLB1a的表达模式分析及功能初步鉴定*[J]. 华北农学报, 2025, 40(5): 161-170.
[13]	何斐, 汤鑫塬, 李竹梅, 褚洪龙. 花魔芋AkTCP家族基因鉴定及表达分析[J]. 华北农学报, 2025, 40(5): 35-46.
[14]	姜昊, 张琳杰, 蔡嘉茹, 王雪晴, 卢杰, 周毅, 朱玉磊, 王升星. 小麦HBD基因家族全基因组鉴定及功能分析[J]. 华北农学报, 2025, 40(4): 29-40.
[15]	李生梅, 李静, 庞博, 张茹, 耿世伟, 陈佳林, 宋武, 高文伟. *棉花微管蛋白Togaram1基因的克隆及表达分析*[J]. 华北农学报, 2025, 40(4): 76-84.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

杜梨PbKRP2序列特征与表达特性分析

Analysis of Sequence Characteristics and Expression Patterns of PbKRP2 in Pyrus betulaefolia

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 12

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价