NaCl胁迫下切花芍药秀兰·邓波尔的生理生化特性

doi:10.7668/hbnxb.20195391

摘要/Abstract

摘要：

为探究NaCl胁迫下切花芍药的生理生化特性,以切花芍药品种秀兰·邓波尔开花级数为一级的花枝为试验材料,采用水培的方法,用不同浓度NaCl(0,0.2%,0.4%,0.6%)溶液处理,观测花枝的表型变化,用DAB、NBT染色和测定相应的生理生化反应,了解切花芍药秀兰耐盐潜力,探究切花芍药的耐盐机理。结果表明:切花芍药秀兰在0.2%轻度盐胁迫时枝条表型无明显变化,0.4%盐胁迫叶片开始出现轻微黄化卷曲现象,0.6%盐胁迫下盐害症状更加明显,叶片卷曲皱缩。盐胁迫下芍药叶片含水量,叶绿素含量均随着盐浓度的升高显著下降;电解质渗透率,脯氨酸含量显著上升;高浓度盐胁迫导致芍药叶片活性氧($\mathrm{O}^{\bar{.}}_{2}$和 H₂O₂)积累显著,多种抗氧化酶活性显著上调。综上所述,0.2%盐胁迫对切花芍药秀兰影响较小,但0.4%及以上的盐浓度超出秀兰的耐受范围;切花芍药秀兰主要通过增加渗透调节物质和提高抗氧化酶的含量来应对盐胁迫。

关键词: 芍药, NaCl胁迫, 耐盐性, 生理生化指标

Abstract:

To explore the physiological and biochemical characteristics of cut peony under NaCl stress,the flower branches of the cut peony variety Shirley Temple with the first level of flowering series as the test material were treated with different concentrations of NaCl(0,0.2%,0.4%,0.6%)by hydroponics method.The phenotype changes of the flower branches were observed,and the corresponding physiological and biochemical reactions were measured by DAB and NBT staining,so as to understand the salt tolerance potential of the cut peony Shirley.To explore the salt tolerance mechanism of cut flower peony.The results showed that there was no significant change in branch phenotype of peony Xiulan under 0.2% salt stress,the leaf became slightly yellowed and curled under 0.4% salt stress,and the leaf curled and wrinkled under 0.6% salt stress.Under salt stress,leaf water content and chlorophyll content decreased significantly with the increase of salt concentration.Electrolyte permeability and proline content were significantly increased.The accumulation of reactive oxygen species($\mathrm{O}^{\bar{.}}_{2}$ and H₂O₂)and the activity of various antioxidant enzymes were significantly up-regulated under high concentration salt stress.In summary,0.2% salt stress had little effect on Xiulan,but 0.4% and above salt concentration exceeded the tolerance range of Xiulan.The cut peony Xiulan can cope with salt stress mainly by increasing the content of osmoregulatory substances and antioxidant enzymes.

Key words: Peony, NaCl stress, Salt tolerance, Physiological and biochemical index

中图分类号:

S682.12

孟静柔, 陈菊, 郭艳超, 李晓奇, 滕仁艳, 侯宇航, 李永伟. NaCl胁迫下切花芍药秀兰·邓波尔的生理生化特性[J]. 华北农学报, 2025, 40(S1): 171-177. doi: 10.7668/hbnxb.20195391.

MENG Jingrou, CHEN Ju, GUO Yanchao, LI Xiaoqi, TENG Renyan, HOU Yuhang, LI Yongwei. Physiological and Biochemical Characteristics of Cut Peony Shirley Temple under NaCl Stress[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 171-177. doi: 10.7668/hbnxb.20195391.

http://www.hbnxb.net/CN/Y2025/V40/IS1/171

图/表 9

图1 不同NaCl浓度下芍药花枝的表型

Fig.1 Phenotypes of peony branches at different NaCl concentrations

图2 不同浓度盐处理对芍药叶片相对含水量的影响不同字母表示处理间差异显著(P<0.05),图4-7,9同。

Fig.2 Effect of different concentration of salt treatment on relative water content of Paeonia lactiflora leaves Different letters on the column indicated significant difference between treatments(P<0.05),the same as Fig.4-7,9.

图3 试验期间每日无菌水补量

Fig.3 Daily sterile water supplement during the experiment

图4 不同浓度盐处理对芍药脯氨酸含量的影响

Fig.4 Effect of different concentration of salt treatment on the content of proline in Paeonia lactiflora

图5 不同浓度盐处理对芍药电解质渗透率的影响

Fig.5 Effect of different concentration of salt treatment on electrolyte permeability of Paeonia lactiflora

图6 不同浓度NaCl处理对芍药Na⁺含量的影响

Fig.6 Effect of different concentrations of NaCl on Na⁺ content of Paeonia lactiflora

图7 不同浓度盐处理对芍药叶绿素含量的影响

Fig.7 Effect of different concentration of salt treatment on chlorophyll content of Peony

图8 不同浓度NaCl处理下芍药叶片DAB和NBT染色情况

Fig.8 DAB and NBT staining of Paeonia lactiflora leaves treated with different concentrations of NaCl

图9 不同浓度NaCl处理对芍药抗氧化酶活性的影响

Fig.9 Effects of different concentrations of NaCl on the activity of antioxidant enzymes in Paeonia lactiflora

参考文献 32

[1]	王慧娟, 符真珠, 李艳敏, 蒋卉, 高杰, 张和臣. 观赏芍药杂交育种研究进展[J]. 北方园艺, 2021(16):144-149.doi:10.11937/bfyy.20204948.
	Wang H J, Fu Z Z, Li Y M, Jiang H, Gao J, Zhang H C. Research progress on the crossbreeding of ornamental herbaceous peony[J]. Northern Horticulture, 2021(16):144-149.
[2]	沈春宇, 田代科, 曾宋君. 芍药组植物的分布和栽培格局及其促成栽培研究现状[J]. 植物资源与环境学报, 2012, 21(4):100-107.
	Shen C Y, Tian D K, Zeng S J. Distribution and cultivation patterns and research status of forcing cultivation of Sect.Paeonia DC.species[J]. Journal of Plant Resources and Environment, 2012, 21(4):100-107.
[3]	Zhao D Q, Zhang X Y, Wang R, Liu D, Sun J, Tao J. Herbaceous peony tryptophan decarboxylase confers drought and salt stresses tolerance[J]. Environmental and Experimental Botany, 2019, 162:345-356.doi:10.1016/j.envexpbot.2019.03.013. URL
[4]	Yang M Y, Luo Z S, Gao S N, Belwal T, Wang L, Qi M, Ban Z J, Wu B, Wang F Z, Li L. The chemical composition and potential role of epicuticular and intracuticular wax in four cultivars of table grapes[J]. Postharvest Biology and Technology, 2021, 173:111430.doi:10.1016/j.postharvbio.2020.111430. URL
[5]	蒋昌华, 叶康, 高燕, 宋垚, 莫健彬, 赵广琦, 奉树成. 盐胁迫对13种芍药品种部分生理指标的影响研究[J]. 西北林学院学报, 2018, 33(2):70-74.doi:10.3969/j.issn.1001-7461.2018.02.11.
	Jiang C H, Ye K, Gao Y, Song Y, Mo J B, Zhao G Q, Feng S C. Effects of salt stress on physiological indexes in 13 varieties of herbaceous peony[J]. Journal of Northwest Forestry University, 2018, 33(2):70-74.
[6]	张津沪. 芍药切花的贮藏期限及预处理对采后品质的影响[D]. 北京: 中国农业大学, 2022.
	Zhang J H. Effects of cold storage on flower postharvest quality of cut peony[D]. Beijing: China Agricultural University, 2022.
[7]	张殿忠, 汪沛洪, 赵会贤. 测定小麦叶片游离脯氨酸含量的方法[J]. 植物生理学通讯, 1990, 26(4):62-65.doi:10.13592/j.cnki.ppj.1990.04.030.
	Zhang D Z, Wang P H, Zhao H X. Determination of the content of free proline in wheat leaves[J]. Plant Physiology Communications, 1990, 26(4):62-65.
[8]	上海市植物生理学会. 现代植物生理学实验指导指南[M]. 北京: 科学出版社, 1999:12-14,16-18.
	Shanghai Plant Physiology Society. Guide to modern plant physiology experiments[M]. Beijing: Science Press, 1999:12-14,16-18.
[9]	豆昕桐, 王英杰, 王华忠, 岳洁瑜. 耐盐和盐敏感型小麦品种对NaCl胁迫的生理响应及耐盐性差异[J]. 生态学报, 2021, 41(12):4976-4992.doi:10.5846/stxb202005251330.
	Dou X T, Wang Y J, Wang H Z, Yue J Y. Physiological response and tolerance difference of two wheat varieties to NaCl stress[J]. Acta Ecologica Sinica, 2021, 41(12):4976-4992.
[10]	Pan T W, Zhang J, He L, Hafeez A, Ning C C, Cai K Z. Silicon enhances plant resistance of rice against submergence stress[J]. Plants, 2021, 10(4):767.doi:10.3390/plants10040767. URL
[11]	Zhao C Z, Zhang H, Song C P, Zhu J K, Shabala S. Mechanisms of plant responses and adaptation to soil salinity[J]. The Innovation, 2020, 1(1):100017.doi:10.1016/j.xinn.2020.100017. URL
[12]	Chen Z H, Pottosin I I, Cuin T A, Fuglsang A T, Tester M, Jha D, Zepeda-Jazo I, Zhou M X, Palmgren M G, Newman I A, Shabala S. Root plasma membrane transporters controlling K⁺/Na⁺ homeostasis in salt-stressed barley[J]. Plant Physiology, 2007, 145(4):1714-1725.doi:10.1104/pp.107.110262. URL
[13]	El-Shabrawi H, Kumar B, Kaul T, Reddy M K, Singla-Pareek S L, Sopory S K. Redox homeostasis,antioxidant defense,and methylglyoxal detoxification as markers for salt tolerance in Pokkali rice[J]. Protoplasma, 2010, 245(1):85-96.doi:10.1007/s00709-010-0144-6. URL
[14]	王琪, 袁燕波, 于晓南. 盐碱胁迫下2个芍药品种生理特性及耐盐碱性研究[J]. 河北农业大学学报, 2013, 36(6):52-60.doi:10.13320/j.cnki.jauh.2013.06.011.
	Wang Q, Yuan Y B, Yu X N. Study on physiological characteristics and saline-alkali tolerance for two cultivars of herbaceous peony[J]. Journal of Hebei Agricultural University, 2013, 36(6):52-60.
[15]	Lin Z, Li Y, Zhang Z J, Liu X L, Hsu C C, Du Y Y, Sang T, Zhu C, Wang Y B, Satheesh V, Pratibha P, Zhao Y, Song C P, Tao W A, Zhu J K, Wang P C. A RAF-SnRK2 kinase cascade mediates early osmotic stress signaling in higher plants[J]. Nature Communications, 2020,11:613.doi:10.1038/s41467-020-14477-9.
[16]	王晓玲, 杨洁, 陈维峰, 刘芳, 袁宗胜. 盐胁迫对3种灌木植物生长及生理特性的影响[J]. 亚热带农业研究, 2024, 20(2):135-144.doi:10.13321/j.cnki.subtrop.agric.res.2024.02.008.
	Wang X L, Yang J, Chen W F, Liu F, Yuan Z S. Effects of salt stress on the growth status and physiological characteristics of three shrubby plants[J]. Subtropical Agriculture Research, 2024, 20(2):135-144.
[17]	Munns R. Comparative physiology of salt and water stress[J]. Plant,Cell & Environment, 2002, 25(2):239-250.doi:10.1046/j.0016-8025.2001.00808.x.
[18]	Zhang Y, Xu J, Li R F, Ge Y R, Li Y F, Li R L. Plants' response to abiotic stress:mechanisms and strategies[J]. International Journal of Molecular Sciences, 2023, 24(13):10915.doi:10.3390/ijms241310915. URL
[19]	Gong Z Z, Xiong L M, Shi H Z, Yang S H, Herrera-Estrella L R, Xu G H, Chao D Y, Li J R, Wang P Y, Qin F, Li J, Ding Y L, Shi Y T, Wang Y, Yang Y Q, Guo Y, Zhu J K. Plant abiotic stress response and nutrient use efficiency[J]. Science China Life Sciences, 2020, 63(5):635-674.doi:10.1007/s11427-020-1683-x.
[20]	闵筱筱, 杜雨露, 滕云, 王志勇, 李佩玲. 盐胁迫对菊花紫燕翻飞生长及生理特性的影响[J]. 山东农业科学, 2024, 56(6):47-54.doi:10.14083/j.issn.1001-4942.2024.06.007.
	Min X X, Du Y L, Teng Y, Wang Z Y, Li P L. Effects of salt stress on growth and physiological characteristics of chrysanthemum Ziyanfanfei[J]. Shandong Agricultural Sciences, 2024, 56(6):47-54.
[21]	周丹, 李海燕, 王秀军, 李庆卫. 外源褪黑素对盐胁迫下银杏幼苗渗透调节和抗氧化能力的影响[J]. 应用生态学报, 2024, 35(2):431-438.doi:10.13287/j.1001-9332.202402.001.
	Zhou D, Li H Y, Wang X J, Li Q W. Effects of exogenous melatonin on the osmotic regulation and antioxidant capacity of Ginkgo biloba seedlings under salt stress[J]. Chinese Journal of Applied Ecology, 2024, 35(2):431-438.
[22]	冯鑫炜, 乔帅帅, 曹丹妮, 刘露, 杨秀清. 盐分胁迫对白刺幼苗生长及生理指标的影响[J]. 山西农业科学, 2015, 43(8):927-931.doi:10.3969/j.issn.1002-2481.2015.08.04.
	Feng X W, Qiao S S, Cao D N, Liu L, Yang X Q. Effect of salt stress on growth and physiological indexes of introduced Nitraria tangutorum seedlings[J]. Journal of Shanxi Agricultural Sciences, 2015, 43(8):927-931.
[23]	刘国花. 植物抗盐机理研究进展[J]. 安徽农业科学, 2006, 34(23):6111-6112.doi:10.13989/j.cnki.0517-6611.2006.23.017.
	Liu G H. Research progress in mechanism of plant resistance to salt stress[J]. Journal of Anhui Agricultural Sciences, 2006, 34(23):6111-6112.
[24]	Hniličková H, Hnilička F, Orsák M, Hejnák V. Effect of salt stress on growth,electrolyte leakage,Na⁺ and K⁺ content in selected plant species[J]. Plant, Soil and Environment, 2019, 65(2):90-96.doi:10.17221/620/2018-pse.
[25]	Li C, Mur L A J, Wang Q H, Hou X C, Zhao C Q, Chen Z M, Wu J Y, Guo Q. ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity[J]. Frontiers in Plant Science, 2022, 13:979956.doi:10.3389/fpls.2022.979956. URL
[26]	高永生, 王锁民, 宫海军, 赵志光, 张承烈. 盐胁迫下植物离子转运的分子生物学研究[J]. 草业学报, 2003, 12(5):18-25.doi:10.3321/j.issn:1004-5759.2003.05.003.
	Gao Y S, Wang S M, Gong H J, Zhao Z G, Zhang C L. Molecular biological research on the transport of ions in plants under salt stress[J]. Acta Prataculturae Sinica, 2003, 12(5):18-25.
[27]	李峰, 高宏云, 张翀, 张宝英, 马建富, 郭娜, 白苇, 方爱国, 杨志敏, 李源. 盐胁迫对燕麦生长及生理指标的影响[J]. 作物杂志, 2024(6):140-146.doi:10.16035/j.issn.1001-7283.2024.06.019.
	Li F, Gao H Y, Zhang C, Zhang B Y, Ma J F, Guo N, Bai W, Fang A G, Yang Z M, Li Y. Effects of salt stress on growth and physiological indexes of oat[J]. Crops, 2024(6):140-146.
[28]	Sapre S, Gontia-Mishra I, Tiwari S. Klebsiella sp. confers enhanced tolerance to salinity and plant growth promotion in oat seedlings(Avena sativa)[J]. Microbiological Research, 2018, 206:25-32.doi:10.1016/j.micres.2017.09.009. URL
[29]	钱永强, 孙振元, 韩蕾, 巨关升, 刘俊祥, 曹丽. 野牛草叶片活性氧及其清除系统对水分胁迫的响应[J]. 生态学报, 2010, 30(7):1920-1926.doi:10.20103/j.stxb.2010.07.031.
	Qian Y Q, Sun Z Y, Han L, Ju G S, Liu J X, Cao L. Response of reactive oxygen and its scavenging system in leaves of Buchloe dactyloides(Nutt.) engelm to water stress[J]. Acta Ecologica Sinica, 2010, 30(7):1920-1926.
[30]	Bonnecarrère V, Borsani O, Díaz P, Capdevielle F, Blanco P, Monza J. Response to photoxidative stress induced by cold in Japonica rice is genotype dependent[J]. Plant Science, 2011, 180(5):726-732.doi:10.1016/j.plantsci.2011.01.023. pmid: 21421424
[31]	Wu B, Munkhtuya Y, Li J J, Hu Y N, Zhang Q, Zhang Z W. Comparative transcriptional profiling and physiological responses of two contrasting oat genotypes under salt stress[J]. Scientific Reports, 2018, 8(1):16248.doi:10.1038/s41598-018-34505-5. pmid: 30389990
[32]	曹慧, 王孝威, 韩振海, 许雪峰, 王永章. 水分胁迫诱导平邑甜茶叶片衰老期间内肽酶与活性氧累积的关系[J]. 中国农业科学, 2004, 37(2):274-279.doi:10.3321/j.issn:0578-1752.2004.02.019.
	Cao H, Wang X W, Han Z H, Xu X F, Wang Y Z. Relationship between changes of endopeptidases activity and active oxygen in Malus hupehensis leaves during senescence induced by water stress[J]. Scientia Agricultura Sinica, 2004, 37(2):274-279.

选择文件类型/文献管理软件名称

选择包含的内容