UV-B辐射对不同发育时期辣椒主要果实品质的影响

doi:10.7668/hbnxb.20193870

摘要/Abstract

摘要：

为了探讨辣椒果实对UV-B辐射的生理应激反应机制,进一步阐述高UV-B辐射地区蔬菜果实品质劣变的机理,以紫外敏感型品种华美105和耐紫外型品种乐都长辣椒的幼果、青果、红果的果实为试材,研究0(CK),2,4,6 h不同UV-B辐射时长(剂量)对果实生长发育、还原型保护及营养品质相关的物质含量的影响。结果表明:随着辐射时间的延长,华美105青果期和红果期的单果质量、纵横径显著降低,从而抑制其果实的生长;2个品种各个生育期的果实中保护性物质Vc、总酚含量随着处理时长的延长逐渐增加,可溶性蛋白含量逐渐下降,其中6 h大幅度提高了Vc、总酚的含量,可溶性蛋白含量下降幅度最大,且华美105各处理下Vc、总酚、可溶性蛋白含量的变化幅度较乐都长辣椒要大;2个品种果实各生育期营养风味品质物质可溶糖随着辐射时间的延长其含量表现为先升高后降低的趋势,纤维素含量表现为先降低后升高的趋势。总之,UV-B对2个品种幼果期生长发育指标的影响不大,高剂量UV-B可以显著抑制紫外敏感型品种青果期和红果期的生长,并能显著增加2个品种Vc、总酚、纤维素等物质的含量,显著减小可溶性蛋白、可溶性糖含量;而低剂量的UV-B能大幅度提升可溶性糖含量,且上升或下降幅度为紫外敏感型品种>耐紫外性品种。因此,低于1/4致死剂量的UV-B能促进果实品质,高于1/2致死剂量的UV-B使得辣椒果实品质开始劣变。

关键词: 辣椒果实, UV-B辐射, 不同生育期, 果实品质

Abstract:

In order to investigate the physiological stress response mechanism of pepper fruits to UV-B radiation and further elaborate the mechanism of fruit quality deterioration in vegetables in high UV-B radiation areas,the young fruits,green fruits and red fruits of UV-sensitive variety Huamei 105 and UV-resistant variety Ledu long pepper were selected as test materials. The effects of different UV-B radiation duration (dose)of 0 (CK),2,4 and 6 h on fruit growth and development,prototype protection and nutrient quality related substances content were studied. The results showed that with the extension of radiation time,the single fruit weight and transverse diameter of Huamei 105 decreased significantly at the green and red fruit stages,which inhibited the fruit growth. The contents of protective substances Vc and total phenol in fruits of the two varieties at each growth stage increased gradually as the extension of treatment time,while the contents of soluble protein decreased gradually. The contents of Vc and total phenol significantly increased after 6 h,and the contents of soluble protein decreased the most. The contents of Vc,total phenol and soluble protein of Huamei 105 changed greater than those of Ledu long pepper. With the extension of radiation time,the content of soluble sugar showed a trend of first increasing and then decreasing,while the content of cellulose showed a trend of first decreasing and then increasing. In conclusion,UV-B had little effect on the indexes in the young fruit stage of the two varieties. High-dose UV-B could significantly inhibit the growth of the UV-sensitive varieties at the green and red fruit stages,and significantly increase the contents of Vc,total phenol,cellulose and other substances in the two varieties,and significantly reduce the contents of soluble protein and soluble sugar. However,low dose UV-B could significantly increase the soluble sugar content,and the increase or decrease amplitude was higher in UV-sensitive varieties than in UV-resistant varieties. Therefore,lower than 1/4 lethal dose of UV-B can promote fruit quality,and higher than 1/2 lethal dose of UV-B can make pepper fruit quality start to deteriorate.

Key words: Pepper fruit, UV-B radiation, Different growth periods, Quality of fruit

陈艳, 张文娟, 邓昌蓉, 侯全刚, 刘敏, 邵登魁. UV-B辐射对不同发育时期辣椒主要果实品质的影响[J]. 华北农学报, 2023, 38(S1): 180-187. doi: 10.7668/hbnxb.20193870.

CHEN Yan, ZHANG Wenjuan, DENG Changrong, HOU Quangang, LIU Min, SHAO Dengkui. Effects of UV-B Radiation on Main Fruit Quality of Capsicum annuum at Different Developmental Stages[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 180-187. doi: 10.7668/hbnxb.20193870.

http://www.hbnxb.net/CN/Y2023/V38/IS1/180

图/表 8

表1 试验设计方案

Tab.1 Test design scheme

处理Treament	描述Description
CK(对照)	自然光不进行UV-B照射
T1	致死剂量的1/6(60/85 μW/cm²、2 h/d)
T2	致死剂量的1/4(60/85 μW/cm²、4 h/d)
T3	致死剂量的1/2(60/85 μW/cm²、6 h/d)

图1 UV-B照射对辣椒果实平均单果质量的影响注:a、b、c、d表示在0.05水平上同一品种同一果实发育阶段不同处理间差异显著。

Fig.1 Effect of UV-B irradiation on average fruit weight of pepper fruit Note:a, b, c and d indicated significant differences among different treatments at the same fruit development stage of the same variety at the level of 0.05.

图2 UV-B辐射对辣椒果实纵径和横径的影响

Fig.2 Effects of UV-B radiation on longitudinal and transverse diameter of pepper fruit

图3 UV-B辐射对辣椒果实抗坏血酸含量的影响

Fig.3 Effect of UV-B radiation on ascorbic acid content in pepper fruit

图4 UV-B辐射对辣椒果实总酚含量的影响

Fig.4 Effect of UV-B radiation on total phenolic content of pepper fruit

图5 UV-B辐射对辣椒果实可溶性蛋白含量的影响

Fig.5 Effect of UV-B radiation on soluble protein content of pepper fruit

图6 UV-B辐射对辣椒果实可溶性糖含量的影响

Fig.6 Effect of UV-B radiation on soluble sugar content of pepper fruit

图7 UV-B辐射对辣椒果实纤维素含量的影响

Fig.7 Effect of UV-B radiation on cellulose content of pepper fruit

参考文献 22

[1]	姚晓芹. 青藏高原东缘几种树苗对增强紫外线-B和氮供应的响应[D]. 成都: 中国科学院成都生物研究所, 2007.
	Yao X Q. Responses of several saplings in the eastern margin of Qinghai-Tibet Plateau to enhanced UV-B and nitrogen supply[D]. Chengdu: Chengdu Institute of Biology,Chinese Academy of Sciences, 2007.
[2]	周秀骥, 徐祥德, 颜鹏, 翁永辉, 王建林. 2000年春季沙尘暴动力学特征[C]// 推进气象科技创新加快气象事业发展——中国气象学会2004年年会论文集(下册), 2004:466-467.
	Zhou X J, Xu X D, Yan P, Wang Y H, Wang J L. Dynamic characteristics of sandstorms in spring 2000[C]// Promoting the Innovation of Meteorological Science and Technology and Accelerating the Development of Meteorological Undertakings--Proceedings of the 2004 Annual Meeting of the Chinese Meteorological Society (Volume 2), 2004:466-467.
[3]	Teramura A H. Effects of ultraviolet-B radiation on the growth and yield of crop plants[J]. Physiologia Plantarum, 1983, 58(3):415-427.doi:10.1111/j.1399-3054.1983.tb04203.x. URL
[4]	叶超. 青海循化线辣椒带富撒拉人家[N]. 西部时报,2008-11-25(013).
	Ye C. Fusaraja in Qinghai pepper belt of circulating line[N]. Western Times,2008-11-25(013).
[5]	Bieza K, Lois R. An Arabidopsis mutant tolerant to lethal ultraviolet-B levels shows constitutively elevated accumulation of flavonoids and other phenolics[J]. Plant Physiology, 2001, 126(3):1105-1115.doi:10.1104/pp.126.3.1105. pmid: 11457961
[6]	王静, 冯娜娜, 王丹, 姜静, 王太霞, 李景原. UV-B辐射对紫花苜蓿幼苗品质和抗氧化性的影响[J]. 食品科技, 2016, 41(6):56-60.doi:10.13684/j.cnki.spkj.2016.06.014.
	Wang J, Feng N N, Wang D, Jiang J, Wang T X, Li J Y. Effects of UV-B radiation on quality and antioxidant properties of alfalfa(Medicago sativa L.) sprouts[J]. Food Science and Technology, 2016, 41(6):56-60.
[7]	刘敏, 李荣贵, 范海, 杜桂彩. UV-B辐射对烟草光合色素和几种酶的影响[J]. 西北植物学报, 2007, 27(2):291-296.doi:10.3321/j.issn:1000-4025.2007.02.014.
	Liu M, Li R G, Fan H, Du G C. Effects of enhanced UV-B radiation on photosynthetic pigments and some enzymes in tobacco[J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(2):291-296.
[8]	傅敏杰, 朱志玉, 朱月清, 王云霞, 朱祝军, 吴建国. UV-B补光对大棚茄子果实农艺性状和抗病性的影响[J]. 北方园艺, 2022(1):65-71. doi:10.11937/bfyy.20212980.
	Fu M J, Zhu Z Y, Zhu Y Q, Wang Y X, Zhu Z J, Wu J G. Effects of UV-B supplementary light on agronomic characters and disease resistance of eggplant in greenhouse[J]. Northern Horticulture, 2022(1):65-71.
[9]	陈修德. UV-B辐射强度和不同薄膜对设施桃树花果发育特性的影响[D]. 泰安: 山东农业大学, 2009.doi:10.7666/d.Y1539260.
	Chen X D. Effects of UV-B radiation intensity and different films on flower and fruit development characteristics of protected peach trees[D]. Taian: Shandong Agricultural University, 2009.
[10]	周开兵, 李世军, 袁孟玲, 岳堃. 杧果成年树在增强UV-B辐射处理下的损伤与抗氧化响应[J]. 园艺学报, 2019, 46(7):1279-1289. doi:10.16420/j.issn.0513-353x.2018-0448.
	Zhou K B, Li S J, Yuan M L, Yue K. Injuries and the responses on antioxidation of adult mango trees under the treatments of enhanced UV-B radiation treatment[J]. Acta Horticulturae Sinica, 2019, 46(7):1279-1289. doi: 10.16420/j.issn.0513-353x.2018-0448
[11]	袁孟玲, 岳堃, 王红, 郭钰柬, 周开兵. 增强UV-B辐射对芒果成年树光合作用及其产量与常规品质的影响[J]. 南方农业学报, 2018, 49(5):930-937. doi:10.3969/j.issn.2095-1191.2018.05.15.
	Yuan M L, Yue K, Wang H, Guo Y J, Zhou K B. Effects of enhanced UV-B radiation treatment on photosynthesis,yield and conventional quality of adult mango tree[J]. Journal of Southern Agriculture, 2018, 49(5):930-937.
[12]	Dobrikova A G, Apostolova E L. Damage and protection of the photosynthetic apparatus from UV-B radiation. Ⅱ. Effect of quercetin at different pH[J]. Journal of Plant Physiology, 2015, 184:98-105.doi:10.1016/j.jplph.2015.06.008. pmid: 26282614
[13]	孙莹. 紫外线-B(UV-B)辐射增强对葡萄果实品质的影响[D]. 杨凌: 西北农林科技大学, 2009.
	Sun Y. The effect of enhanced ultraviolet-B radiation on the quality of grape[D]. Yangling: Northwest A&F University, 2009.
[14]	刘景玲. UV-B辐射和干旱对丹参生物量及酚酸类成分含量的影响[D]. 杨凌: 西北农林科技大学, 2014.
	Liu J L. Effects of UV-B radiation and drought on biomass and phenolic acids content of Salvia miltiorrhiza Bge.[D]. Yangling: Northwest A&F University, 2014.
[15]	王红, 杨成坤, 岳堃, 郭钰柬, 周开兵. 不同UV-B辐射对杧果树果实品质及其抗氧化的影响[J]. 热带作物学报, 2020, 41(2):275-283.doi: 10.3969/j.issn.1000-2561.2020.02.010.
	Wang H, Yang C K, Yue K, Guo Y J, Zhou K B. Effect of mango trees fruit quality and antioxidant under different UV-B radiation treatments[J]. Chinese Journal of Tropical Crops, 2020, 41(2):275-283.
[16]	陈岚. 补充紫外线-B照射对不结球白菜生长与品质及生理特性的影响[D]. 南京: 南京农业大学, 2007.doi: 10.7666/d.Y1215088.
	Chen L. Effect of supplementary UV-B irradiation on growth,quality and physiological characteristics of non-heading Chinese cabbage[D]. Nanjing: Nanjing Agricultural University, 2007.
[17]	殷红, 郭巍, 毛晓艳, 张涛. 增强的UV-B辐射对水稻光合作用的影响[J]. 作物杂志, 2009(4):41-45. doi:10.3969/j.issn.1001-7283.2009.04.012.
	Yin H, Guo W, Mao X Y, Zhang T. Effect of enhanced UV-B radiation on physiological response of rice[J]. Crops, 2009(4):41-45.
[18]	郑丽萍, 方媛, 刘滨, 任树勇, 彭励. UV-B辐射增强对甘草幼苗生长及生理代谢的影响[C]// 第十届全国植物结构与生殖生物学学术研讨会会议报告摘要,海口, 2012:52-53.
	Zheng L P, Fang Y, Liu B, Ren S Y, Peng L. Effects of enhanced UT-B radiation on growth and physiological metabolism of Licorice seedlings[C]// The Tenth National Symposium on Plant Structure and Reproductive Biology,Haikou, 2012:52-53.
[19]	杨乐, 杨俊枫, 侯智霞, 宫中志, 王冲, 史文君. UV-B对不同发育时期离体蓝莓主要果实品质及相关酶活性的影响[J]. 西北植物学报, 2015, 35(12):2477-2482. doi:10.7606/j.issn.1000-4025.2015.12.2477.
	Yang L, Yang J F, Hou Z X, Gong Z Z, Wang C, Shi W J. Effects of UV-B treatment on the major quality of blueberry and related enzyme activities in different developmental stages[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(12):2477-2482.
[20]	裴惠娟, 张满效, 安黎哲. 非生物胁迫下植物细胞壁组分变化[J]. 生态学杂志, 2011, 30(6):1279-1286.doi:10.13292/j.1000-4890.2011.0235.
	Pei H J, Zhang M X, An L Z. Changes of plant cell wall components under abiotic stresses:a review[J]. Chinese Journal of Ecology, 2011, 30(6):1279-1286.
[21]	金克霞, 江泽慧, 刘杏娥, 杨淑敏, 田根林, 马建锋. 植物细胞壁纤维素纤丝聚集体结构研究进展[J]. 材料导报, 2019, 33(17):2997-3002.doi: 10.1186/1754-6834-4-41.
	Jin K X, Jiang Z H, Liu X E, Yang S M, Tian G L, Ma J F. Research advance in cellulose fibril aggregates structure of plant cell wall[J]. Materials Reports, 2019, 33(17):2997-3002.
[22]	曲颖, 王弋博, 冯虎元, 程佳强, 李文建, 安黎哲. UV-B辐射对豌豆伸长生长和细胞壁多糖组分的影响[J]. 辐射研究与辐射工艺学报, 2012, 30(5):303-308.
	Qu Y, Wang Y B, Feng H Y, Cheng J Q, Li W J, An L Z. Effects of UV-B radiation on stems elongation and cell wall polysaccharides of pea seedlings[J]. Journal of Radiation Research and Radiation Processing, 2012, 30(5):303-308.

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