植物中谷氧还蛋白研究进展

doi:10.7668/hbnxb.20191815

摘要/Abstract

摘要： 谷氧还蛋白（GRX）是一种小分子热稳定的蛋白质，广泛存在于动物、植物和微生物中。其中在动物和人体内研究较多，在植物中研究相对较少，在不同植物中谷氧还蛋白基因的数量和种类也不尽相同。谷氧还蛋白通常含有一个保守的CXXC/S活性位点，根据其活性位点的氨基酸序列可将其分为3类：CPYC型、CGFS型和CC型。其中，前2种为真核生物所共有，而CC类型为陆生植物所特有，且数量最多。谷氧还蛋白发挥作用时有单巯基和双巯基机制，可以在谷胱甘肽分子的帮助下还原二硫键或蛋白质谷胱甘肽加合物。谷氧还蛋白、谷胱甘肽、NADPH和谷胱甘肽还原酶共同组成了谷氧还蛋白系统。谷氧还蛋白在植物中与硫氧还蛋白系统、抗坏血酸谷胱甘肽循环系统等共同参与细胞的氧化还原状态的维持以及各类细胞进程的调节，并且在植物细胞抵抗生物以及非生物的氧化胁迫中起着十分重要的作用。另外，谷氧还蛋白还在植物生长发育、氮代谢、铁硫簇形成等方面具有重要作用。本研究就植物中谷氧还蛋白的命名、分类、作用机制以及在植物体内发挥的功能进行汇总，以期对其有更深入的了解。

关键词: 植物, 谷氧还蛋白, 作用机制, 功能, 生物胁迫

Abstract: Glutaredoxin(GRX)is a small moleculer and heat-stable protein, which is widely found in animals, plants and microorganisms. Among them, there are more studies in animals and humans, and relatively few studies in plants. The number and types of glutaredoxin genes in different plants are not the same. Glutaredoxins usually contain a conservative CXXC/S active site, which can be divided into three types according to the amino acid sequence of the active site:CPYC type, CGFS type and CC type. Among them, the first two types are shared by eukaryotes, while the CC type is unique to terrestrial plants and has the largest number. Glutaredoxin has single sulfhydryl and double sulfhydryl mechanism when it works, and it can reduce disulfide bonds or protein glutathione adducts with the help of glutathione molecules. Glutaredoxin, glutathione, NADPH and glutathione reductase together form the glutathione system. Glutaredoxin in plants, together with the thioredoxin system, the ascorbate glutathione cycle system, and so on. Participate in the maintenance of the redox state of the cell and the regulation of various cell processes, and it resists biological and non-biological oxidation in plant cells. It plays a very important role in coercion. In addition, glutaredoxin also plays an important role in plant growth and development, nitrogen metabolism and iron-sulfur cluster formation. This article briefly introduces the naming, classification, mechanism of action and functions of glutaredoxin in plants in order to have a deeper understanding of it.

Key words: Plants, Glutaredoxin, Mechanism of action, Function, Biological stress

中图分类号:

Q945

张硕, 王硕, 韩胜芳, 王冬梅. 植物中谷氧还蛋白研究进展[J]. 华北农学报, 2021, 36(S1): 202-209. doi: 10.7668/hbnxb.20191815.

ZHANG Shuo, WANG Shuo, HAN Shengfang, WANG Dongmei. The Research Progress of Glutaredoxin in Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 202-209. doi: 10.7668/hbnxb.20191815.

http://www.hbnxb.net/CN/Y2021/V36/IS1/202

参考文献

[1] 刘聪,董腊嫒,林建中,刘选明.逆境胁迫下植物体内活性氧代谢及调控机理研究进展[J].生命科学研究,2019,23(3):253-258.doi:10.16605/j.cnki.1007-7847.2019.03.012.
Liu C,Dong L A,Lin J Z,Liu X M.Researchéadvances on regulation mechanism of reactive oxygen species metabolism under stresses[J]. Life Science Research,2019,23(3):253-258.
[2] Dutilleul C,Garmier M,Noctor G,Mathieu C,Chétrit P,Foyer C H,de Paepe R. Leaf mitochondria modulate whole cell redox homeostasis,set antioxidant capacity,and determine stress resistance through altered signaling and diurnal regulation[J]. The Plant Cell,2003,15(5):1212-1226.doi:10.1105/tpc.009464.
[3] Foyer C H,Noctor G. Redox homeostasis and antioxidant signaling:a metabolic interface between stress perception and physiological responses[J]. The Plant Cell,2005,17(7):1866-1875.doi:10.1105/tpc.105.033589.
[4] Rouhier N. Plant glutaredoxins:pivotal players in redox biology and iron-sulphur centre assembly[J]. New Phytologist,2010,186(2):365-372.doi:10.1111/j.1469-8137.2009.03146.x.
[5] Rouhier N,Gelhaye E,Jacquot J P. Plant glutaredoxins:still mysterious reducing systems[J]. Cellular and Molecular Life Sciences CMLS,2004,61(11):1266-1277.doi:10.1007/s00018-004-3410-y.
[6] Lemaire S D. The glutaredoxin family in oxygenic photosynthetic organisms[J]. Photosynthesis Research,2004,79(3):305-318.doi:10.1023/B:PRES.0000017174.60951.74.
[7] Rouhier N,Couturier J,Jacquot J P. Genome-wide analysis of plant glutaredoxin systems[J]. Journal of Experimental Botany,2006,57(8):1685-1696.doi:10.1093/jxb/erl001.
[8] Xing S,Lauri A,Zachgo S. Redox regulation and flower development:a novel function for glutaredoxins[J]. Plant Biology,2006,8(5):547-555.doi:10.1055/s-2006-924278.
[9] Bell G,Polaina J,Tamarit J,De La Torre M A,Rodríguez-Manzaneque M T,Ros J,Herrero E. Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein[J]. The Journal of Biological Chemistry,2002,277(40):37590-37596.doi:10.1074/jbc.M201688200.
[10] Tamarit J,Belli G,Cabiscol E,Herrero E,Ros J. Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin[J]. Journal of Biological Chemistry,2003,278(28):25745-25751.doi:10.1074/jbc.M303477200.
[11] Fernandes A P,Fladvad M,Berndt C,Andrésen C,Lillig C H,Neubauer P,Sunnerhagen M,Holmgren A,Vlamis-Gardikas A. A novel monothiol glutaredoxin(Grx4)from Escherichia coli can serve as a substrate for thioredoxin reductase[J]. Journal of Biological Chemistry,2005,280(26):24544-24552.doi:10.1074/jbc.M500678200.
[12] Rouhier N,Lemaire S D,Jacquot J P. The Role of glutathione in photosynthetic organisms:emerging functions for glutaredoxins and glutathionylation[J]. Annual Review of Plant Biology,2008,59(1):143-166.doi:10.1146/annurev.arplant.59.032607.092811.
[13] Gutsche N,Thurow C,Zachgo S,Gatz C. Plant-specific CC-type glutaredoxins:functions in developmental processes and stress responses[J]. Biological Chemistry,2015,396(5):495-509.doi:10.1515/hsz-2014-0300.
[14] Malik W A,Wang X G,Wang X L,Shu N,Cui R F,Chen X G,Wang D L,Lu X K,Yin Z J,Wang J J,Ye W W. Genome-wide expression analysis suggests glutaredoxin genes response to various stresses in cotton[J]. International Journal of Biological Macromolecules,2020,153:470-491.doi:10.1016/j.ijbiomac.2020.03.021.
[15] Wang Z,Xing S P,Birkenbihl R P,Zachgo S.Conserved functions of Arabidopsis and rice CC-type glutaredoxins in flower development and pathogen response[J]. Molecular Plant,2009,2(2):323-335.doi:10.1093/mp/ssn078.
[16] Mora-Garcia S,Stolowicz F G,Wolosiuk R A. Redox signal transduction in plant metabolism[M].American Cancer Society,2018,22:150-186.doi:10.1002/9780470988640.ch6.
[17] Rey P,Taupin-Broggini M,Couturier J,Vignols F,Rouhier N. Is there a role for glutaredoxins and BOLAs in the perception of the cellular iron status in plants?[J]. Frontiers in Plant Science,2019,10:712.doi:10.3389/fpls.2019.00712.
[18] Feng Y G,Zhong N,Rouhier N,Hase T,Kusunoki M,Jacquot J P,Jin C W,Xia B. Structural insight into poplar glutaredoxin C1 with a bridging iron-sulfur cluster at the active site[J]. Biochemistry,2006,45(26):7998-8008.doi:10.1021/bi060444t.
[19] Noguera V,Walker O,Rouhier N,Jacquot J P,Krimm I,Lancelin J M.NMR reveals a novel glutaredoxin-glutaredoxin interaction interface[J]. Journal of Molecular Biology,2005,353(3):629-641.doi:10.1016/j.jmb.2005.08.035.
[20] Noguera-Mazon V,Lemoine J,Walker O,Rouhier N,Salvador A,Jacquot J P,Lancelin J M,Krimm I.Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer[J]. Journal of Biological Chemistry, 2006,281(42):31736-31742.doi:10.1016/S0021-9258(19)84088-7.
[21] Xing S P,Rosso M G,Zachgo S. ROXY1,a member of the plant glutaredoxin family,is required for petal development in Arabidopsis thaliana[J]. Development,2005,132(7):1555-1565.doi:10.1242/dev.01725.
[22] Xing S P,Zachgo S. ROXY1 and ROXY2,two Arabidopsis glutaredoxin genes,are required for anther development[J]. The Plant Journal,2008,53(5):790-801.doi:10.1111/j.1365-313X.2007.03375.x.
[23] Wu Q Y,Yang J,Cheng N H,Hirschi K D,White F F,Park S.Glutaredoxins in plant development,abiotic stress response,and iron homeostasis:From model organisms to crops[J]. Environmental and Experimental Botany,2017,139:91-98.doi:10.1016/j.envexpbot.2017.04.007.
[24] Delorme-Hinoux V,Bangash S A K,Meyer A J,Reichheld J P. Nuclear thiol redox systems in plants[J]. Plant Science,2016,243:84-95.doi:10.1016/j.plantsci.2015.12.002.
[25] Liu S J,Fu H,Jiang J M,Chen Z J,Gao J D,Shu H R,Zhang S,Yang C W,Liu J. Overexpression of a CPYC-Type Glutaredoxin,OsGrxC2.2,causes abnormal embryos and an increased grain weight in rice[J]. Frontiers in Plant Science,2019,10:848.doi:10.3389/fpls.2019.00848.
[26] Zaffagnini M,Michelet L,Massot V,Trost P,Lemaire S D.Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii reveals the unique properties of a chloroplastic CGFS-type glutaredoxin[J]. Journal of Biological Chemistry,2008,283(14):8868-8876.doi:10.1074/jbc.M709567200.
[27] Trnka D,Engelke A D,Gellert M,Moseler A,Hossain M F,Lindenberg T T,Pedroletti L,Odermatt B,de Souza J V,Bronowska A K,Dick T P,Mühlenhoff U,Meyer A J,Berndt C,Lillig C H. Molecular basis for the distinct functions of redox-active and FeS-transfering glutaredoxins[J]. Nature Communications,2020,11(1):3445.doi:10.1038/s41467-020-17323-0.
[28] Foyer C H,Noctor G. Ascorbate and glutathione:the heart of the redox hub[J]. Plant Physiology, 2011,155(1):2-18.doi:10.1104/pp.110.167569.
[29] Laporte D,Olate E,Salinas P,Salazar M,Jordana X,Holuigue L. Glutaredoxin GRXS13 plays a key role in protection against photooxidative stress in Arabidopsis[J]. Journal of Experimental Botany,2012,63(1):503-515.doi:10.1093/jxb/err301.
[30] Martins L,Knuesting J,Bariat L,Dard A,Freibert S A,Marchand C,Young D,Dung NHT,Voth W,de Bures A,Saez-Vasquez J,Lemaire S D,Roland L,Messens J,Scheibe R,Reichheld J P,Riondet C. Redox modification of the Fe-S glutaredoxin GRXS17 activates holdase activity and protects plants from heat stress[J]. Plant Physiology,2020,184:676-692.doi:10.1104/pp.20.00906.
[31] Son S,Kim H,Lee K S,Kim S,Park S R.Rice glutaredoxin GRXS15 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae and Fusarium fujikuroi[J]. Biochemical and Biophysical Research Communications,2020,533(4):1385-1392.doi:10.1016/j.bbrc.2020.10.027.
[32] Ning X,Sun Y,Wang C C,Zhang W L,Sun M H,Hu H T,Liu J Z,Yang L. A rice CPYC-type Glutaredoxin OsGRX20 in protection against bacterial blight,methyl viologen and salt stresses[J]. Frontiers in Plant Science,2018,9:111.doi:10.3389/fpls.2018.00111.
[33] Hou J Y,Zhang Q H,Zhou Y,Ahammed G J,Zhou Y H,Yu J Q,Fang H,Xia X J. Glutaredoxin GRXS16 mediates brassinosteroid-induced apoplastic H₂O₂ production to promote pesticide metabolism in tomato[J]. Environmental Pollution,2018,240:227-234.doi:10.1016/j.envpol.2018.04.120.
[34] 冯军.水杨酸介导的草莓抗白粉病的分子调控机制[D].北京:北京林业大学,2020.
Feng J. The molecular regulatory mechanism of salicylic acid-primed strawberry resistance against Podosphaera aphanis[D].Beijing:Beijing Forestry University,2020.
[35] Li T T,Li M Z,Jiang Y M,Duan X W.Genome-wide identification,characterization and expression profile of glutaredoxin gene family in relation to fruit ripening and response to abiotic and biotic stresses in banana(Musa acuminata)[J]. International Journal of Biological Macromolecules,2021,170:636-651.doi:10.1016/j.ijbiomac.2020.12.167.
[36] Ströher E,Grassl J,Carrie C,Fenske R,Whelan J,Millar A H. Glutaredoxin S15 is involved in Fe-S cluster transfer in mitochondria influencing lipoic acid-dependent enzymes,plant growth,and arsenic tolerance in Arabidopsis[J]. Plant Physiology, 2016,170(3):1284-1299.doi:10.1104/pp.15.01308.
[37] Uhrig J F,Huang L J,Barghahn S,Willmer M,Thurow C,Gatz C.CC-type glutaredoxins recruit the transcriptional co-repressor TOPLESS to TGA-dependent target promoters in Arabidopsis thaliana[J]. Biochimica et Biophysica Acta(BBA)-Gene Regulatory Mechanisms, 2017,1860(2):218-226.doi:10.1016/j.bbagrm.2016.11.001.
[38] Ma B J,Suo Y F,Zhang J,Xing N N,Gao Z Q,Lin X F,Zheng L L,Wang Y C. Glutaredoxin like protein(RtGRL1)regulates H₂O₂and Na⁺ accumulation by maintaining the glutathione pool during abiotic stress[J]. Plant Physiology and Biochemistry,2021,159:135-147.doi:10.1016/j.plaphy.2020.11.040.
[39] Di X R,Zheng F,Norton G J,Beesley L,Zhang Z L,Lin H,Zhi S L,Liu X C,Ding Y Z.Physiological responses and transcriptome analyses of upland rice following exposure to arsenite and arsenate[J]. Environmental and Experimental Botany,2021,183:104366.doi:10.1016/j.envexpbot.2020.104366.
[40] Verma P K,Verma S,Pande V,Mallick S,Deo Tripathi R,Dhankher O P,Chakrabarty D. Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana[J]. Frontiers in Plant Science,2016,7:740.doi:10.3389/fpls.2016.00740.
[41] Ding S C, He F Y, Tang W L, Du H W,Wang H W.Identification of maize CC-Type glutaredoxins that are associated with response to drought stress[J]. Genes,2019,10(8):810.doi:10.3390/genes10080610.
[42] Kumar A,Dubey A K,Kumar V,Ansari M A,Narayan S,Meenakshi,Kumar S,Pandey V,Shirke P A,Pande V,Sanyal I. Over-expression of chickpea glutaredoxin(CaGrx)provides tolerance to heavy metals by reducing metal accumulation and improved physiological and antioxidant defence system[J]. Ecotoxicology and Environmental Safety,2020,192:110252.doi:10.1016/j.ecoenv.2020.110252.
[43] Ruan M B,Yang Y L,Li K M,Guo X,Wang B,Yu X L,Peng M. Identification and characterization of drought-responsive CC-type glutaredoxins from cassava cultivars reveals their involvement in ABA signalling[J]. BMC Plant Biology, 2018,18(1):329.doi:10.1186/s12870-018-1528-6.
[44] 杨义伶.木薯CC-type MeGRXs及MeDREB1D 基因抗旱功能研究[D].武汉:华中农业大学,2016.
Yang Y L.Funtional analysis of CC-type MeGRXs and MeDREB1D genes from cassava in drought tolerance[D].Wuhan:Huazhong Agricultural University,2016.
[45] Hu Y,Wu Q Y,Peng Z,Sprague S A,Wang W,Park J,Akhunov E,Jagadish K S V,Nakata P A,Cheng N H,Hirschi K D,White F F,Park S. Silencing of OsGRXS17 in rice improves drought stress tolerance by modulating ROS accumulation and stomatal closure[J] .Scientific Reports,2017,7(1):15950.doi:10.1038/s41598-017-16230-7.
[46] Wu Q Y,Yang J,Cheng N H,Hirschi K D,White F F,Park S.Glutaredoxins in plant development,abiotic stress response,and iron homeostasis:From model organisms to crops[J]. Environmental and Experimental Botany,2017,139:91-98.doi:10.1016/j.envexpbot.2017.04.007.
[47] Liu Y,Xu C J,Zhu Y F,Zhang L N,Chen T Y,Zhou F,Chen H,Lin Y J. The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice[J]. Journal of Integrative Plant Biology,2018,60(2):173-188.doi:10.1111/jipb.12614.
[48] 姜良宝.梅花响应低温胁迫的生理变化和基因表达模式研究[D].北京:北京林业大学,2020.
Jiang L B.Physiological changes and gene expression pattern in response to low temperature stress in Prunus mume[D].Beijing:Beijing Forestry University,2020.
[49] Ehrary A,Rosas M,Carpinelli S,Davalos O,Cowling C,Fernandez F,Escobar M. Glutaredoxin AtGRXS8 represses transcriptional and developmental responses to nitrate in Arabidopsis thaliana roots[J]. Plant Lirect,2020,4(6):e00227.doi:10.1002/pld3.227.
[50] Huang L J,Li N,Thurow C,Wirtz M,Hell R,Gatz C. Ectopically expressed glutaredoxin ROXY19 negatively regulates the detoxification pathway in Arabidopsis thaliana[J]. BMC Plant Biology,2016,16(1):200.doi:10.1186/s12870-016-0886-1.
[51] Rey P,Taupin-Broggini M,Couturier J,Vignols F,Rouhier N. Is There a role for glutaredoxins and BOLAs in the perception of the cellular iron status in plants?[J]. Frontiers in Plant Science,2019,10:712.doi:10.3389/fpls.2019.00712.
[52] I n igo S,Durand A N,Ritter A,Le Gall S,Termathe M,Klassen R,Tohge T,De Coninck B,Van Leene J,De Clercq R,Cammue B P,Fernie A R,Gevaert K,De Jaeger G,Leidel S A,Schaffrath R,Van Lijsebettens M,Pauwels L,Goossens A.Glutaredoxin GRXS17 associates with the cytosolic Iron-Sulfur cluster assembly pathway[J]. Plant Physiology,2016,172(2):858-873.doi:10.1104/pp.16.00261.
[53] Mühlenhoff U,Braymer J J,Christ S,Rietzschel N,Uzarska M A,Weiler B D,Lill R. Glutaredoxins and iron-sulfur protein biogenesis at the interface of redox biology and iron metabolism[J]. Biological Chemistry,2020,401(12):1407-1428.doi:10.1515/hsz-2020-0237.
[54] Daniel T,Faruq H M,Laura Magdalena J,Manuela G,Christopher Horst L. Role of GSH and Iron-Sulfur glutaredoxins in iron metabolism-review[J]. Molecules,2020,25(17):3860.doi:10.3390/molecules25173860.
[55] Zannini F,Moseler A,Bchini R,Dhalleine T,Meyer A J,Rouhier N,Couturier J. The thioredoxin-mediated recycling of Arabidopsis thaliana GRXS16 relies on a conserved C-terminal cysteine[J]. Biochimica et Biophysica Acta(BBA)-General Subjects,2019,1863(2):426-436.doi:10.1016/j.bbagen.2018.11.014.

选择文件类型/文献管理软件名称

选择包含的内容