基于SLAF-seq及BSA技术的甜瓜抗蔓枯病QTL定位分析

doi:10.7668/hbnxb.20191702

摘要/Abstract

摘要： 为研究甜瓜蔓枯病（GSB）抗病基因在甜瓜染色体上的定位情况，对甜瓜蔓枯病抗病亲本P181、感病亲本P01及其F₂遗传分离群体进行蔓枯病抗性鉴定，根据F₂蔓枯病发病情况，选择2个亲本及F₂中极端抗病和极端感病的子代各30个，提取DNA并分别构建抗病和感病基因混池，利用SLAF-seq和BSA技术进行蔓枯病抗病基因QTL定位分析。测序共开发得到188 022个SLAF标签，分析过滤掉低质量的SNP位点后，获得5 676个高质量的SNP标记。采用SNP-index关联分析方法，在甜瓜染色体上获得2个与GSB抗性紧密关联的候选区域，一个定位于Chr01上的9 837 447~11 028 838 bp区间，区间大小为1.19 Mb；另一个定位于Chr04上的33 135 224~33 993 540 bp区间，区间大小为0.86 Mb。共有218个基因定位在关联区域内，依据基因功能注释分析，推测可能与甜瓜蔓枯病抗病相关的候选基因有15个，包括NBS-LRR基因1个，E3泛素蛋白连接酶合成基因5个，RPM1相关蛋白基因1个，锌指蛋白相关基因1个，NAC结构域蛋白基因1个，钙依赖蛋白激酶2个，凝集素相关受体蛋白基因3个，RING-H2 finger蛋白基因1个。研究结果将为甜瓜蔓枯病的抗性基因研究提供参考。

关键词: 甜瓜, 蔓枯病, 抗病基因, SALF-seq, QTL

Abstract: In order to investigate the resistant genes location of gummy stem blight(GSB) on chromosomes of melon, the resistance parent P181, susceptible parent P01 and F₂ progeny were used for GSB resistance genes identification. According to the disease incidence of GSB of F₂,two parents, 30 high-resistance and 30 high-suspect F₂ generations were choice to isolated DNA, in order to construct resistant and susceptible pools. The QTL locations were applicated by SLAF-seq and BSA technique. A total of 188 022 SLAF-labels were obtained, after low-quality SNP sites filterted out, 5 676 high-quality SNP markers were developed. Two GSB-resistance QTLs were identified on chromosomes of melon by the methods of SNP-index, which were located in Chr01 and Chr04, with 1.19 Mb (on the region of 9 837 447-11 028 838 bp) and 0.86 Mb (on the region of 33 135 224-33 993 540 bp) interval, respectively. A total of 218 genes were analysis in QTLs. Furthermore, according to the gene function annotations, 15 candidate genes were speculated with the relationship of GSB resistance, including 1 gene of NBS-LRR, 5 genes of E3 ubiquitin-protein ligase, 1 gene of RPM1-interacting protein, 1 gene of zinc finger protein, 1 gene of NAC domain-containing protein, 2 genes of calcium-dependent protein kinase, 3 genes of L-type lectin-domain containing receptor kinase, 1 gene of RING-H2 finger protein. The results will support reference of the basis of resistance genes of GSB.

Key words: Melon, Gummy stem blight, Resistance genes, SLAF-seq, QTL

中图分类号:

Q78
S652.03

高天一, 郝芳敏, 臧全宇, 马二磊, 黄芸萍, 王毓洪. 基于SLAF-seq及BSA技术的甜瓜抗蔓枯病QTL定位分析[J]. 华北农学报, 2021, 36(2): 40-45. doi: 10.7668/hbnxb.20191702.

GAO Tianyi, HAO Fangmin, ZANG Quanyu, MA Erlei, HUANG Yunping, WANG Yuhong. QTL Analysis of Gummy Stem Blight in Melon by SALF-seq and BSA Technique[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 40-45. doi: 10.7668/hbnxb.20191702.

http://www.hbnxb.net/CN/Y2021/V36/I2/40

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