新铁炮百合朱丽叶器官差异基因表达分析

doi:10.7668/hbnxb.2017.05.005

摘要/Abstract

摘要： 为了探明17个候选基因在新铁炮百合朱丽叶花、叶、鳞片3个器官中的表达情况，利用实时荧光定量PCR技术，对其相对表达量进行了研究。结果表明，根据表达情况，17个候选基因可以分为5类，CL10636.Contig1_All、CL6300.Contig2_All、CL7951.Contig5_All、Unigene8314_All、Unigene19279_All和CL1306.Contig1_All 6个在花中显著高表达，分别与糖转运蛋白-类SWEET4基因、赤霉素调控蛋白基因、线粒体伴侣基因、光依赖性短下胚轴基因、1-脱氧葡萄糖-5-磷酸合成酶基因、牻牛儿基牻牛儿基焦磷酸合成酶基因高度同源；CL9292.Contig2_All、CL212.Contig5_All、Unigene1800_All、CL3468.Contig1_All、CL4520.Contig5_All、Unigene4212_All、CL4079.Contig1_All和Unigene10210_All 8个在叶中显著高表达，分别与GDSL酯酶/脂酶基因、类黄酮O-甲基转移酶、脂氢过氧化物裂解酶基因、4-羟基-3-甲基-2-丁烯基二磷酸合成酶基因、多酚氧化酶基因、类葡苷露聚糖4-β-甘露糖转移酶基因、左旋海松烷合成酶基因和反式罗勒烯合成酶基因高度同源；CL2405.Contig1_All在鳞片中显著高表达，功能未知；CL1114.Contig2_All在花和叶中同时显著高表达，与3-酮酯酰CoA-硫解酶高度同源；CL1772.Contig2_All为非器官差异表达基因，与△24-甾醇还原酶基因高度同源。此结果可为今后研究这些候选基因的功能奠定基础，对百合分子育种具有重要意义。

关键词: 百合, 差异基因, 实时荧光定量PCR, 器官

Abstract: To understand the expression levels of 17 candidate genes in flower,leaf and scale of Lilium formolongi Jilius,Real time quantitative PCR was conducted. The results showed that the 17 candidate genes could be divided into five groups.CL10636.Contig1_All,CL6300.Contig2_All,CL7951. Contig5_All,Unigene8314_All,Unigene19279_All and CL1306.Contig1_All significantly highly expressed in flowers,which were highly homologous with Bidirectional sugar transporter SWEET4-like gene,cold-regulated gibberellin-regulated protein gene bidirectional,mitochondrial chaperone gene,Lightlight-dependent short hypocotyls 4-like gene,1-deoxyxylulose-5-phosphate synthase gene,geranylgeranyl pyrophosphate synthase gene. CL9292.Contig2_All,CL212.Contig5_All,Unigene1800_All,CL3468. Contig1_All,CL4520.Contig5_All,Unigene4212_All,CL4079.Contig1_All and Unigene10210_All eight significantly highly expressed in leaves,which were highly homologous with GDSL esterase/lipase gene,flavonoid O-methyltransferase gene,hydroperoxide lyase gene,4-hydroxy-3-methylbut-2-en-1-yl diphosphate gene,polyphenol oxidase gene,glucomannan 4-beta-mannosyltransferase 1-like gene,Levopimaradiene synthase gene and trans-ocimene synthase gene. CL2405.Contig1_All significantly highly expressed in scales,function unknown. CL1114.Contig2_All significantly highly expressed in both flowers and leaves,highly homologous with 3-ketoacyl-CoA thiolase gene. CL1772.Contig2_All,homologous with delta-(24)-sterol reductase,was non-organ specific gene. The results are valuable for the functional study of those genes and will be benefit for the molecular breeding of lily.

Key words: Lily, Differentially expressed genes, Real-time q-PCR, Organs

中图分类号:

S682.29
Q78

杜方, 王婷, 樊俊苗, 张浩宇. 新铁炮百合朱丽叶器官差异基因表达分析[J]. 华北农学报, 2017, 32(5): 25-30. doi: 10.7668/hbnxb.2017.05.005.

DU Fang, WANG Ting, FAN Junmiao, ZHANG Haoyu. Analysis of Differentially Expressed Genes in Organs of Lilium formolongi Julius[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 25-30. doi: 10.7668/hbnxb.2017.05.005.

http://www.hbnxb.net/CN/Y2017/V32/I5/25

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