二倍体马铃薯SSR遗传图谱的构建

doi:10.7668/hbnxb.2017.05.018

华北农学报 ›› 2017, Vol. 32 ›› Issue (5): 117-123. doi: 10.7668/hbnxb.2017.05.018

所属专题： 薯类作物；

二倍体马铃薯SSR遗传图谱的构建

朱云¹, 郭晓¹, 孙小磊¹, 马伟清¹, 董道峰¹, 郭宝太², 杨煜¹, 杨晓慧¹, 李广存¹

1. 山东省农业科学院蔬菜花卉研究所, 山东省设施蔬菜分子生物学重点实验室, 山东济南 250100;
2. 青岛农业大学生命科学学院, 山东青岛 266109

收稿日期:2017-08-09 出版日期:2017-10-28
通讯作者: 杨晓慧(1980-),女,山东聊城人,助理研究员,博士,主要从事马铃薯分子育种研究。
作者简介:朱云(1987-),女,安徽马鞍山人,硕士,主要从事马铃薯抗病分子生物学研究。
基金资助:
山东省自然科学基金项目（ZR2014YL014；ZR2016CM27）；山东省农业科学院青年科研基金项目（2014QNZ03）

Construction of an SSR-based Genetic Linkage Map of Diploid Potato

ZHU Yun¹, GUO Xiao¹, SUN Xiaolei¹, MA Weiqing¹, DONG Daofeng¹, GUO Baotai², YANG Yu¹, YANG Xiaohui¹, LI Guangcun¹

1. Vegetable and Flower Institute, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory for Biology of Greenhouse Vegetables, Jinan 250100, China;
2. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China

Received:2017-08-09 Published:2017-10-28

摘要/Abstract

摘要： 为了丰富马铃薯分子标记，定位青枯病抗性基因位点，利用具有抗青枯病遗传背景的二倍体马铃薯亲本USW5337.3（C）和772102.37（E）及其123份杂交一代无性系，进行了马铃薯SSR遗传图谱的构建。根据马铃薯全基因组序列设计了864对SSR引物，其中187对在亲本间表现出差异，135对（72.2%）能够在杂交一代中扩增出清晰的条带，最终构建了一张由12个连锁群组成，包含135个SSR标记的马铃薯分子标记遗传图谱。12个连锁群总长度为948.4 cM，标记间平均间距7.03 cM，含有5个标记偏分离区域。不仅为马铃薯饱和分子遗传图谱的构建提供新的SSR标记，对于马铃薯抗青枯病QTL定位、基因克隆和分子标记辅助选择也具有重要的参考意义。

关键词: 二倍体, 马铃薯, SSR, 连锁图谱

Abstract: In order to enrich molecular markers and position the bacterial wilt resistant gene loci in potato,an SSR-based genetic linkage map was constructed from a diploid population consisting of 123 individuals derived from a cross between two potato diploid clones (C and E) which harborer resistance to Ralstonia solanacearum in this study.A total of 864 simple sequence repeat primer pairs were designed according to the potato genome sequence and used to screen parents C and E for polymorphisms.Of 187 polymorphic loci,72.2% showed clear amplifiable and scorable bands in the F₁ population.Then a total of 135 locus were mapped to 12 linkage groups,of which the cumulative length was 948.4 cM,with an average marker interval of 7.03 cM,and 5 segregation distortion regions in three linkage groups.This study provided a set of new SSR markers for genetic map construction and increased saturation of map in potato.The relatively high density map may provide a useful reference for further identification,mapping of quantitative trait loci (QTL) and cloning of potato bacterial wilt resistance genes and marker-assisted breeding.

Key words: Diploid, Potato, Simple sequence repeat, Linkage map

中图分类号:

S532.03

朱云, 郭晓, 孙小磊, 马伟清, 董道峰, 郭宝太, 杨煜, 杨晓慧, 李广存. 二倍体马铃薯SSR遗传图谱的构建[J]. 华北农学报, 2017, 32(5): 117-123. doi: 10.7668/hbnxb.2017.05.018.

ZHU Yun, GUO Xiao, SUN Xiaolei, MA Weiqing, DONG Daofeng, GUO Baotai, YANG Yu, YANG Xiaohui, LI Guangcun. Construction of an SSR-based Genetic Linkage Map of Diploid Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 117-123. doi: 10.7668/hbnxb.2017.05.018.

http://www.hbnxb.net/CN/Y2017/V32/I5/117

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