大豆ZF-HD转录因子<em>GmZHD1</em>的克隆及表达分析

doi:10.7668/hbnxb.2017.02.001

摘要/Abstract

摘要： 为了揭示大豆ZF-HD基因的生物学功能，通过PCR的方法，从大豆栽培豆科丰1号中克隆了ZF-HD转录因子Glyma.02g040100，命名为GmZHD1。生物信息学分析表明，该基因的cDNA全长为888 bp，编码295个氨基酸，GmZHD1蛋白分子量约为32.64 kDa，等电点为7.69；序列分析表明，GmZHD1含有ZF-HD家族保守的锌指结构域和同源异形盒结构域，GmZHD1与FtHB2蛋白序列一致性最高，为44.4%；亚细胞定位结果显示GmZHD1定位于细胞核；荧光定量PCR结果表明，GmZHD1在大豆不同组织中都有表达，其中，花、种子和叶片中表达较高。此外，将GmZHD1基因连接到植物过表达载体pBA002上，为进一步研究大豆GmZHD1基因的功能奠定了基础。

关键词: 大豆, GmZHD1, 亚细胞定位, RT-PCR

Abstract: The ZF-HD (Zinc finger homeodomain)transcription factors,which belong to Homeobox protein family,are unique to plant kingdom and play an important role in the development of flowers and leaves in plants.The Glyma.02g040100 gene,a ZF-HD transcription factor,was isolated from soybean cultivar Kefeng No.1 by PCR method and was named as GmZHD1.Bioinformatics analysis showed that this gene contained 888 bp encoding 295 amino acid residues and the molecular weight of GmZHD1 protein was 32.64 kDa with a theoretical isoelectric point (pI)7.69.Sequence analysis indicated that GmZHD1 possessed two conserved domains,a zinc finger domain and a homeobox domain,and shares highest sequence similarity with FtHB2 (44.4%);The result of subcellular localization indicated that GmZHD1 was located in nucleus;And RT-PCR results showed that GmZHD1 expressed in all tissues detected,and with relatively higher transcript expression levels in flower,seed and leaf.In addition,the GmZHD1 was linked to the plant over-expression vector pBA002 successfully,providing foundation for further study on the function of GmZHD1 in soybean.

Key words: Glycine max, GmZHD1, Subcellular localization, RT-PCR

中图分类号:

S565.1
Q78

张晋玉, 晁毛妮, 杜弘杨, 喻德跃, 黄方. 大豆ZF-HD转录因子GmZHD1的克隆及表达分析[J]. 华北农学报, 2017, 32(2): 1-7. doi: 10.7668/hbnxb.2017.02.001.

ZHANG Jinyu, CHAO Maoni, DU Hongyang, YU Deyue, HUANG Fang. Cloning and Expression Analysis of ZF-HD Transcription Factor GmZHD1 in Glycine max[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 1-7. doi: 10.7668/hbnxb.2017.02.001.

http://www.hbnxb.net/CN/Y2017/V32/I2/1

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大豆ZF-HD转录因子GmZHD1的克隆及表达分析

Cloning and Expression Analysis of ZF-HD Transcription Factor GmZHD1 in Glycine max

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大豆ZF-HD转录因子GmZHD1的克隆及表达分析

Cloning and Expression Analysis of ZF-HD Transcription Factor GmZHD1 in Glycine max

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