摘要： 为了研究微卫星标记在哲罗鱼增殖放流中的应用,采用18对微卫星标记分析了哲罗鱼放流回捕群体(JC)、塔河群体(TH)及放流亲本群体(HT、YZ)的遗传多样性和遗传结构,并对回捕个体进行了鉴定.结果表明,Na、Ne、I、He和H均是HT >JC >TH >YZ,Ho 是YZ群体最高,TH群体最低,这4个群体的遗传多样性均处于中等偏上水平;AMOVA分析表明,4个群体内差异不显著,群体间差异达到显著水平;遗传距离分析显示YZ群体和HT群体的相似度最大为0.718 3,JC群体与其他3个群体的遗传相似度处于中等水平在0.566 5~0.689 5,YZ群体和TH群体的遗传相似性最小为0.495 9.结果显示,微卫星标记能够清晰地显示放流亲本的遗传背景,保证放流子代具有较好的遗传多样性.同时微卫星标记能够区分放流个体和野生个体,可见微卫星标记能够指导哲罗鱼放流工作.为哲罗鱼下一步的放流工作提供了科学依据.

关键词: 微卫星标记, 哲罗鱼, 增殖放流

Abstract: In order to study the application of microsatellites markers in endangered Hucho taimen enhancement and releasing hucho releasing,eighteen microsatellites markers were used to analyze the genetic diversity and structure of release-recapture population (JC),Tahe River population (TH) and released parents' population (HT,YZ) in the present study.The recaptured individuals were also identified.The results indicated that Na,Ne,I,He and H indexes exhibited the same tendency,HT > JC > TH > YZ.The observed heterozygosity (Ho) was highest in YZ population while lowest in TH population.All of these four populations possessed a medium level of genetic diversity.AMOVA analysis showed that there was no significant difference within four groups while extremely significant difference between populations.The genetic similarity was highest between YZ and HT population with the index of 0.718 3,medium-level between JC population and other three populations with the index of 0.566 5-0.689 5, and lowest between YZ and TH population with the index of 0.495 9.The results showed that we could understand the genetic background of the parents and assure the released descendant's polymorphism by microsatellite markers.The released individuals were identified by microsatellite markers.In conclusion,this study provided the basis for the next stage of artificial releasing work.

Key words: Microsatellite markers, Hucho taimen, Enhancement and releasing

中图分类号: 

• S965.1