摘要： 为研究丛枝菌根真菌对黄瓜苗期立枯病的防治效果和相关生理生化变化。以黄瓜品种中农16为材料,播种时接种丛枝菌根真菌,对20 d幼苗人工接种立枯丝核菌。结果表明,接种菌根真菌后能够显著提高黄瓜苗期对立枯病的抗性,降低发病率。黄瓜苗期人工接种立枯丝核菌后菌根苗和非菌根苗发病率分别为16.5%和52.0%,非菌根苗发病严重,病情指数为46.7,接菌病情指数为10,较非接菌苗下降了78.5%,接种菌根真菌提高黄瓜抗病性与改变相关酶活性有关,在病害发生前菌根真菌已经与寄主植物互利共生,提前启动了抵抗病害得相关机制,如提高了保护酶活性,促进植株生长,接种菌根真菌后菌根苗地上鲜质量和地上干质量分别比未接菌处理提高33.10%和25.93%。在病害发生过程中菌根真菌与病原菌互相竞争,抑制病菌对寄主的侵害,减轻细胞膜受损害程度。

关键词: 丛枝菌根真菌, 黄瓜, 立枯病, 增抗机理

Abstract: The cucumber strain Zhongnong 16 were used to screen the effect of arbuscular mycorrhizal fungi on seedling blight of cucumber in solar greenhouse, and related physiological and biochemistry changes were studied.The result indicated that when cucumber seedlings inoculated with mycorrhizal fungi it can significantly enhance the ability of cucumber resistance.After inoculated rhizoctonia solani incidence rates of AM cucumber seedlings and non-AM cucumber seedlings were 16.5% and 52.0%.The mean disease index of cucumber seedling inoculated with AMF was 78.5% lower than that of the control respectively,moreover,there were most obvious the differences between the treatments and the control.Before disease occurrence the sysbiosis of AMF and host plant had formed and the relevant mechanisms of disease resistance had been started.The inoculation of AMF enhanced plant grewth and improved the activity of protect enzyme.During disease occurred AMF and pathogens compete with each other.AMF inhibited the infection of bacteria against host plant.It manifested on activity of Reactive-oxygen-scavenging Enzymes insensitive to the inoculation of pathogens.Degree of membrane lipids peroxidation depressed the damage of cell membrane was smaller.

Key words: Arbuscular mycorrhizal fungi, Cucumber seedling, Blight, Tolerance

中图分类号: 

• S432.4+4