摘要： 为研究利用微生物方法解决养殖水体中氨氮污染的问题，从水产养殖环境中分离出31株氨氮去除菌，并从中筛选出具有较强氨氮去除能力的菌株AD-8，对其脱氮能力进行测定。结果表明，在培养基中氨氮浓度高达200 mg/L时，AD-8菌株对其去除率均达86%以上，15℃下，48 h AD-8对氨氮的去除率可以达到91.2%；在模拟养殖水体中，AD-8氨氮去除率也可达90%。通过形态学特性、生理生化鉴定及16S rDNA序列分析，菌株AD-8初步鉴定为嗜碱假单胞菌（Pseudomonas alcaliphila）。

关键词: 氨氮去除能力, 分离鉴定, 16SrDNA, 嗜碱假单胞菌

Abstract: Microbial method was used to remove ammonia-nitrogen pollutant from the aquaculture water,isolated 31 strains with high Ammonia-nitrogen removing ability from the aquaculture sediment.Among these strains,strain AD-8 showed the highest ability of removing ammonia-nitrogen.In the culture,its removal rate to 200 mg/L ammonia nitrogen was 86%,its removal rate was 91.2% in 48 h in low temperature(15℃).In simulated aquaculture waste water,its net removal efficiency of ammonia nitrogen was 90% after 48 hours.AD-8 was identified as Pseudomonas alcaliphila according to its morphological,physiological and biochemical properties and phylogenetic analysis of its 16S rDNA sequence.

Key words: Ammonia nitrogen removal ability, Isolation and identification, 16S rDNA, Pseudomonas alcaliphila

中图分类号: 

• X172

• X703