拟南芥茉莉酸途径调节植物对根结线虫的防御反应

doi:10.7668/hbnxb.2014.03.042

摘要/Abstract

摘要： 对拟南芥根中的茉莉酸(Jasmonic acid，JA)生物合成和信号转导途径及JA对根结线虫发病机制所起的作用进行了研究。研究发现根结线虫的侵染显著诱导拟南芥根中JA和JA-异亮氨酸共轭物(JA-Ile)的合成，分别为 AOS1、OPR3 和 JAR1 基因调控。根结线虫在JA缺失突变体和JA-Ile缺失突变体(aos1、opr3、jar1)根中的生殖活性明显低于野生型，但是在缺失JA信号转导核心基因的突变体 coi1 和 jin1 的根中同野生型没有显著区别。使用外源JA-Ile可以使根结线虫在 jar1 根中的生殖能力恢复到在野生型根中的水平。这些结果证明，拟南芥根中JA的合成途径为根结线虫侵染所必需。

关键词: 拟南芥, 茉莉酸, 信号通路, 根结线虫, 突变体, 先天免疫

Abstract: Nematode parasites achieve chronic infection by overcoming the innate immunity system of the hosts.In plants, the role of JA to innate immunity is well established in the leaves.The role of JA in defending against parasitic root knot nematode is largely unknown.Here, we showed the root knot nematode Meloidogyne incognita infection induced Allene Oxide Synthase1 (AOS1) and OPR3 (12- oxo-Phytodienoic Acid Reductase 3) dependent JA and Jasmonate Resistant1 (JAR1) dependent JA-Ile biosynthesis.The nematode reproduction ability was significantly lower on the JA-Ile deficient roots(aos1, opr3, jar1) than those of wild types.Two JA signal transduction mutants, the coronatine insensitive1 (coi1) and the JA insensitive1 (jin1), showed wile type susceptibility to the nematode infection.The decreasing nematode reproduction ability on the roots of jar1, specifically missing JA-Ile, could be rescued by exogenous application of JA-Ile.JA synthesis pathway is important in prouss of the nematode infect Arabidopsis root.

Key words: Arabidopsis thaliana, Jasmonic acid, Signaling pathway, Root knot nematode, Mutant, Innate immunity

中图分类号:

Q945

王静, 邬晓红, 其乐木格, 特日格乐, 祁智, 亢燕. 拟南芥茉莉酸途径调节植物对根结线虫的防御反应[J]. 华北农学报, 2014, 29(3): 234-237. doi: 10.7668/hbnxb.2014.03.042.

WANG Jing, WU Xiao-hong, Qilemuge, Terigele, QI Zhi, KANG Yan. JA Mediated Signaling Pathway Regulate Plant Defense Responses of Root Knot Nematode[J]. journal1, 2014, 29(3): 234-237. doi: 10.7668/hbnxb.2014.03.042.

http://www.hbnxb.net/CN/Y2014/V29/I3/234

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