摘要： 为鉴定AtHAK5启动子中的功能域,并进一步确定对应的转录因子。将AtHAK5编码拟南芥高亲和性钾转运体6个不同长度的启动子片段克隆到双元表达载体pORE R1中β-葡萄糖醛酸酶(GUS)报告基因的上游,构成promoter AtHAK5∶GUS融合基因。通过农杆菌侵染野生型拟南芥花序,得到稳定表达该融合基因的T3转基因种子。通过基于GUS活性的组织化学染色法,对这些来自T3转基因种子、经过低钾处理的幼苗进行分析,确定AtHAK5启动子中感应低钾环境的顺式作用元件位于起始转录上游-471～-267范围内。

关键词: 拟南芥, AtHAK5基因, 启动子, 顺式作用元件

Abstract: AtHAK5 encodes a high affinity K +transporter.Six AtHAK5 promoter fragments with different length were cloned into the upstream of β-Glucuronidase reporter gene ( GUS) in pORE R1 binary expression vector, which forms promoter AtHAK5∶∶GUS fusion gene.The T3 generation transformed seeds stably expressing the fusion gene were gotten through Agrobacterium-mediated Arabidopsis transformation with floral dip method.The cis-elements of the AtHAK5 promoter was located at the transcription region of 471-267 thought GUS activity based histochemical staining of the transformed seedlings grown on low potassium condition.This study identified the functional domain within the AtHAK5 promoter,which sets the foundation for further finding of the corresponding transcript factor.

Key words: ARabidopsis, AtHAK5 gene, PRomoteR, Cis-element

中图分类号: 

• Q943

• S184