摘要： 为探索砀山酥梨褐皮芽变果皮褐色形成机理,试验以褐皮芽变果皮为材料,克隆了木质素生物合成相关的PAL2、4CL1、CAD1、PPO1、POD4等5个酶基因3'端序列,并利用Real-time PCR技术,分析各基因在花后25,50,75,100,125,150,175 d等不同时期的表达差异。结果表明,在基因相对表达量各取样时期,锈酥果皮中PAL2、4CL1、CAD1和POD4相对表达量均高于砀山酥梨。砀山酥梨果皮中木质素增量仅与POD4酶基因表达量存在极显著正相关性,而锈酥果皮中木质素增量与PAL2、4CL1、CAD1和POD4酶基因表达量均存在极显著正相关性。PAL2、4CL1、CAD1和POD4酶基因均参与了砀山酥梨芽变锈酥果皮褐色的形成。

关键词: 梨, 褐皮芽变, 基因克隆, 实时定量PCR

Abstract: To study the formation mechanism of the russet mutant of Dangshansuli, the enzyme genes related to the lignin biosynthesis, PAL2,4CL1,CAD1,PPO1,POD4 were cloned and the relative expressions of different genes in the pericarp of Dangshansuli and its russet mutant were analyzed with real -time PCR at 25, 50, 75, 100, 125, 150 and 175 days after full bloom ( DAFB).The results showed that the relative expressions of PAL2,4CL1,CAD1, POD4 and PPO1 genes in pericarp of Xiusu pear were all higher than that of Dangshansuli pear at 25, 50, 75, 100, 125,150 and 175 DAFB.The correlation analysis between the lignin increment and the relative expressions of POD4 enzyme gene exhibited extremely significant differences in the pericarp of Dangshansuli, while that of PAL2, 4 CL1, CAD1 and POD4 enzyme gene in the pericarp of Xiusu.It is believed that PAL2,4CL1,CAD1 and POD4 enzyme genes in biosynthesis of lignin involved in the formation of Xiusu russet skin.

Key words: PyRus, Russet mutant, Gene clone, Real-time PCR

中图分类号: 

• S661.2