摘要： 对沾化冬枣根际解无机磷细菌进行了分离、筛选与鉴定,并对获得的高效溶磷菌进行培养条件及溶磷特性研究。利用透明圈法对沾化冬枣根际土壤中具有溶磷能力的细菌进行初筛;利用改良蒙金娜液体培养基对初筛菌株进行培养后采用钼锑抗比色法测定发酵液的可溶性磷含量,对解磷菌进行复筛;通过形态指标、16S rDNA序列分析对该菌株进行了初步鉴定;同时对该溶磷菌株进行了耐盐试验、液体培养条件下溶磷特性研究、发酵培养基的优化试验,以及促进土壤中有效磷含量增加等一系列研究。结果显示,本试验从沾化冬枣根际土壤中分离出1株耐盐高效解无机磷细菌PSB6,初步鉴定该菌株为土壤杆菌属,能显著增加发酵液和土壤中有效磷的含量。为通过生物途径改善沾化冬枣磷素供应,促进其生长提供了优良的菌株资源。

关键词: 沾化冬枣, 根际土壤, 溶无机磷细菌, 分离和鉴定, 有效磷

Abstract: Phosphate-solubilizing bacterium(PSB) was isolated， screened and identified from the rhizospheric soil of Zhanhua winter jujube( Zizyphus jujuba Mill．) ，and phosphate-dissolving characteristics of PSB were determined．By using the way of clear zone，phosphate-solubilizing bacteria from the rhizospheric soil were preliminarily isolated，and the high effective PSB was further screened by using molybdenum-antimony anti-spectrophotometric method． The PSB was identified by the 16S rDNA sequence analysis combined with morphological characteristics．We carried out the salt-tolerant test，phosphate-solubilizing characteristics of PSB and optimization of fermentation medium in liquid tricalcium phosphate medium． The phosphate-dissolving characteristics of PSB in the soil were carried out under the greenhouse． The results indicated that one strain of high effective PSB was screened and identified as Rhizobium sp． The strain had significant effects on improving the solubilizing phosphate content in the liquid medium and the soil． This study provided excellent strain resource for improving phosphorus supply of Zhanhua winter jujube and promoting its growth．

Key words: Key words: Zhanhua winter jujube, Rhizospheric soil, Phosphate-solubilizing bacteria, Isolation and identification

中图分类号: 

• S432.4