摘要： 以紫娟等15个云南茶树品种为材料,采用高效液相色谱法(HPLC)测定茶叶中的没食子酸(GA)、儿茶素(C)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)、表没食子酸儿茶素(EGC)及表没食子儿茶素没食子酸酯(EGCG)的含量,应用ISSR标记技术分析紫娟的亲缘关系。结果表明:GA、C、ECG、EGCG及儿茶素总量含量在紫娟中较低,而EGC含量在紫娟中含量较高,说明紫娟与其他品种间的儿茶素含量差异较大,在生化上有一定特点。同时,18个ISSR引物共扩增出111个位点,其中89个位点呈现多态性,多态性为80.18%,紫娟与品种间遗传相似系数变幅在0.582～0.800,平均0.693。UPGMA聚类则15个茶树品种分为3组,其中紫娟与云抗27号、73-8、云抗43号等9个品种聚在一起,说明紫娟等15个品种之间的遗传差异较小,遗传基础较窄,亲缘关系比较近。

关键词: 紫娟, 生化特点, ISSR, 遗传关系

Abstract: By using high performance liquid chromatography(HPLC),the gallic acid(GA),catechins(C),epicatechin(EC),epigallocatechin(EGC),epicatechin gallate(ECG) and epigallocatechin gallate(EGCG) contents of 15 tea cultivars from Yunnan Province were measured.Besides,in order to solve the genetic relationship of tea cultivar Zi-juan,the inter-simple sequence repeats(ISSR) markers were used to detect the relationship of 15 tea cultivars including tea cultivar Zi-juan and 14 other cultivars.The results showed that the contents of GA,C,ECG,EGCG and total catechins in tea cultivar Zi-juan were relatively low,but the content of EGC in tea cultivar Zi-juan was relatively high,indicating that there was big difference of various catechin contents among tea cultivar Zi-juan and other tea cultivars,and there exists its biochlemical properties of tea cultivar Zi-juan to some extent.At the same time,the result of ISSR analysis showed that one hundred and eleven DNA fragments among all 15 tea cultivars were amplified by using eighteen reliable ISSR primers,among which 89 DNA bands were polymorphic(PPB=80.18%).The genetic similarity among tea cultivar Zi-juan and 14 other cultivars ranged from 0.582 to 0.800,with a 0.693 average.The cluster analysis presented that these cultivars were divided into three main groups using the unweighted pair-group method based on arithmetic average(UPGMA) based on ISSR molecular marker data,and 10 tea cultivars including Zi-juan,Yunkang 27,Yunkang 43 and so on were grouped together.ISSR analysis results indicated that there existed a small genetic base and fairly close genetic relationships,indicating a narrow gene pool among Zi-juan tea cultivar and 14 other cultivars tested.

Key words: Tea cultivar, Zi-juan, Biochlemical properties, ISSR, Genetic relationship

中图分类号: 

• S571.1