摘要： 扩张蛋白是促进植物细胞壁伸展的一类蛋白质，黄瓜果实膨大过程中扩张蛋白具有重要作用。本研究以发育中的黄瓜果实RNA为试验材料，通过RACE方法克隆了黄瓜Cs-EXP10基因的5’端未知序列，进而获得了该基因的全长，序列分析显示该基因编码287个氨基酸，有扩张蛋白的特征序列。Southern杂交表明Cs-EXP10在基因组中是以单拷贝形式存在的。其表达具有组织特异性，在膨大过程中的果实中表达量高，显示其与果实的膨大有关。在叶和茎组织中也有少量表达，而在根和花中基本不表达。

关键词: 黄瓜, Cs-EXP10基因, 克隆, 表达

Abstract: Expansin is a kind of protein to promote plant cell wall expanding it is important for cucumber fruitenlargement during fruit development. Thisgene coded 287 amino acids after sequence analysis. Expression analysis showed that it expressed highly in expanding fruit and express little in stemsand leaves however almost do not express in roots and flowers which means it correlated with fruit expanding.

Key words: Cucumber, Cs'XP10 gene, Clone, Expression

中图分类号: 

• S642.2