银杏过氧化物酶基因POD1的克隆及表达分析

doi:10.7668/hbnxb.2010.06.009

摘要/Abstract

摘要： 利用RACE技术从银杏中克隆到过氧化物酶基因(GbPOD1,FJ599670)的cDNA全长。GbPOD1基因编码序列为1 092 bp,编码一条长为329Aa的成熟阴离子POD蛋白,预测成熟蛋白分子量为35.8 kDa,等电点为8.10。Southern杂交及进化树分析结果表明,GbPOD1和其他物种的POD源自于相同的祖先,属于植物类型III的POD同工酶编码基因,为多基因家族。RT-PCR分析表明,GbPOD1在银杏的根、茎、叶和果中都有表达,在茎中的相对表达量最高,其次为根和果,而叶部位表达水平最低。植物激素MeJA、金属离子Cu和Cd以及MV和WOU的处理能显著诱导GbPOD1基因的表达。研究结果显示,在银杏叶片中,GbPOD1属于多功能基因,具有潜在的参与重金属污染清除及伤害处理防御方面的功能。

关键词: 银杏, POD1基因, 表达分析

Abstract: The full-length cDNA sequence of novel peroxidasel gene(designed as GbPODl, NCBI accession humber：FJ599670)were isolated from Ginkgo biloba L.The coding region of the gene is l092 bp long, and its deduced mature protein consists of 329 amino acids with a predicted molecular mass of 35.8 kDa and a pI of 8.10.Phylogenetic tree analysis revealed that GbPODl shared the same ancestor with other PODs, could be classified as type III peroxidase.The resuh of southern analysis showed that GbPODl gene was encoded by a multi-gene family in Ginkgo biloba.The expression analysis by RT-PCR showed that GbPODl expressed in a tissue—specific manner in G.biloba.GbPODl was also found to be up-regulated by MeJA, CuS04, CdCl2, MV, and mechanical wounding.These resuhs indicate that the GbPODl has the potential to play a role in response to heavy metal and wounding against foreign attacks, was amultifunction gene.

Key words: Ginkgo biloba L., Peroxidase J, Expression analysis

中图分类号:

S792.95

程华, 李琳玲, 王燕, 程水源. 银杏过氧化物酶基因POD1的克隆及表达分析[J]. 华北农学报, 2010, 25(6): 44-51. doi: 10.7668/hbnxb.2010.06.009.

CHENG Hua, LI Lin-ling, WANG Yan, CHENG Shui-yuan. Molecular Cloning.Characterization and Expression of PODl Gene from Ginkgo blloba L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 44-51. doi: 10.7668/hbnxb.2010.06.009.

http://www.hbnxb.net/CN/Y2010/V25/I6/44

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