摘要： 为了解彩色马铃薯黑美人、拉塞特-01、底西芮、青薯168、水洗红、涩皮红6个品种在DNA分子水平上的遗传差异性,对彩色马铃薯种质鉴定及杂交选育提供依据,试验利用ISSR分子标记技术对其多态性进行了分析.结果表明:12个ISSR适宜引物共扩增出151个条带,其DNA片段长度多为250～2 000 bp,其中多态性条带136个,多态性条带百分率为88.2%.6个彩色马铃薯品种间的遗传距离(GD)为0.38 8～0.6 ,平均为0.539 .以GD值0.55为基准,6个材料聚为3类:第一类为底西芮、青薯168和涩皮红,第二类为拉塞特-01和黑美人,水洗红单独聚为一类,6个彩色马铃薯品种间的亲缘关系存在一定的差异.

关键词: 彩色马铃薯, 品种, 遗传差异, ISSR分析

Abstract: To know the degree of genetic difference among 6 color potato varieties which are Heimeiren,Russet-01,Desiree,Qingshu168,Shuixihong and Sepihong,and provide evidence for potato germplasm evaluation and cross breeding,the polymorphism among the tested plants were analyzed based on ISSR molecular marker. The results showed that 151 bands of ISSR markers were amplified with 12 suitable primers,and the length of DNA fragment were ranged from 250 to 2 000 bp,and 136 bands were polymorphic,and the percentage of polymorphism bands reached 88. 2% . The genetic distance(GD) of 6 tested plants were ranged from 0. 438 8 to 0. 644 4,and the average of genetic distance was 0. 539 4. Using the GD value of 0. 55 as threshold,6 tested plants could be clustered into 3 groups: the first group including Desiree,Qingshu168 and Sepihong,the second groups including Russet-01 and Heimeiren,and the third groups only including Shuixihong,which genetic relationship among 6 color potato varieties are different.

Key words: Color potato, Varieties, Genetic difference, ISSR analysis

中图分类号: 

• S533