西马特罗人工抗原的合成及鼠源多克隆抗血清的制备

doi:10.7668/hbnxb.2010.04.021

华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 97-101. doi: 10.7668/hbnxb.2010.04.021

西马特罗人工抗原的合成及鼠源多克隆抗血清的制备

职爱民, 刘庆堂, 李青梅, 杨苏珍, 胡骁飞, 柴书军, 邓瑞广, 张改平

河南省农业科学院农业部动物免疫学重点实验室, 河南省动物免疫学重点实验室, 河南郑州 450002

收稿日期:2010-07-14 出版日期:2010-08-28
通讯作者: 张改平(1960- )，男，河南内黄人，研究员，博士，博士生导师，中国工程院院士，主要从事动物免疫学和兽药残留研究。
作者简介:职爱民(1976- ),男,河南新乡人,副研究员,博士,主要从事动物营养与免疫研究.
基金资助:
"十一五"国家科技支撑计划(2006BAK02A21)

Synthesis of Cimaterol Artificial Antigen and Development of Its Polyclonal Antiserum

ZHI Ai-min, LIU Qing-tang, LI Qing-mei, YANG Su-zhen, HU Xiao-fei, CHAI Shu-jun, DENG Rui-guang, ZHANG Gai-ping

Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received:2010-07-14 Published:2010-08-28

摘要/Abstract

摘要： 拟合成并鉴定西马特罗(Cimaterol,CIM)人工抗原,通过动物免疫生产亲和力高、特异性好的鼠源CIM多克隆抗血清.通过重氮化方法将西马特罗偶联于载体蛋白BSA和OVA上,分别合成免疫抗原BSA-CIM和包被抗原OVA-CIM,并采用紫外分光光度法和SDS-PAGE进行了鉴定.结果表明,半抗原CIM和载体蛋白偶联成功.动物免疫试验结果显示,6只小鼠效价均达1×10-3以上,且4号小鼠多抗血清抗CIM的IC0为86.9 ng/mL,表明成功获得了高效价、特异性好、亲和力高的的鼠源抗CIM多抗血清.

关键词: 西马特罗, 人工抗原, 合成, 多抗血情

Abstract: The goal of this study was to synthesize artificial antigen of cimaterol and obtain its mice polyclonal antiserum. Artificial antigen BSA-CIM and OVA-CIM were synthesized using diazotization by linking carrier proteins BSA and OVA to CIM. The antigen BSA-CIM and OVA-CIM was identified by ultraviolet scanning，SDS-PAGE. The results showed that the hapten CIM was successfully linked to carrier proteins. The animal immunization results showed that six BALB / c mice indirect ELISA titer against CIM were above 1 × 10^－3 and the IC₅₀ of No. 4 mice was 86. 9 ng /mL and the high-titer，sensitivity CIM polyclonal antiserum has been generated. The artificial antigen of CIM and its mice polyclonal antiserum laid a foundation base for product the monoclonal antibodies and rapid test reagent against CIM.

Key words: Cimaterol, Artificial antigen, Synthesis, Polyclonal antiserum

中图分类号:

S82.2

职爱民, 刘庆堂, 李青梅, 杨苏珍, 胡骁飞, 柴书军, 邓瑞广, 张改平. 西马特罗人工抗原的合成及鼠源多克隆抗血清的制备[J]. 华北农学报, 2010, 25(4): 97-101. doi: 10.7668/hbnxb.2010.04.021.

ZHI Ai-min, LIU Qing-tang, LI Qing-mei, YANG Su-zhen, HU Xiao-fei, CHAI Shu-jun, DENG Rui-guang, ZHANG Gai-ping. Synthesis of Cimaterol Artificial Antigen and Development of Its Polyclonal Antiserum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 97-101. doi: 10.7668/hbnxb.2010.04.021.

http://www.hbnxb.net/CN/Y2010/V25/I4/97

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