摘要： 为提高肉用绵羊的杂交育种效果,采用PCR-RFLP方法检测骨形态发生蛋白受体IB基因(BMPR-IB)在湖羊、小尾寒羊、杜泊绵羊及杜湖、杜寒杂交F1群体中的多态性。结果证明:在湖羊和小尾寒羊中检测到两种基因型(BB和B+),湖羊基因型频率分别为0.909和0.091,小尾寒羊基因型频率分别为0.563和0.437;在杜泊绵羊中只存在一种基因型(++);而在杜湖和杜寒杂交绵羊中发现两种基因型(B+和++),杜湖基因型频率分别为0.875和0.125,杜寒基因型频率分别为0.786和0.214。
关键词:
湖羊,
小尾寒羊,
杜泊绵羊,
杂交羊,
BMPR-IB基因,
PCR-RFLP
Abstract: Polymorphism of Bone morphogenetic protein receptor IB( BMPR-IB) gene in Hu sheep( Hu),Han sheep( Ha),Dorper sheep( D) and their hybrids were detected by PCR-RFLP.The genotype frequencies( BB,B + and + + ) are 0.909,0.091 and 0,0.563,0.437 and 0,0,1.00 and 0,0,0.875 and 0.125( D × Hu),and 0,0.786 and 0.214( D × Ha), respectively．
Key words:
Hu Sheep,
Han Sheep,
Dorper Sheep,
BMPR-IB gene,
PCR-RFLP
中图分类号:
徐小波, 王公金, 窦德宇, 储国良, 赵伟, 花为华, 刘亚柏. 湖羊、小尾寒羊及其杂交后代BMPR-IB基因多态性[J]. 华北农学报, 2010, 25(3): 52-54. doi: 10.7668/hbnxb.2010.03.012.
XU Xiao-bo, WANG Gong-jin, DOU De-yu, CHU Guo-liang, ZHAO Wei, HUA Wei-hua, LIU Ya-bo. Polymorphism of BMPR-IB Gene in Hu Sheep,Han Sheep,Dorper Sheep and Their Hybrids[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 52-54. doi: 10.7668/hbnxb.2010.03.012.