小尾寒羊<i>BMF</i>基因在发情不同阶段卵巢及卵泡发育过程中的表达分析

doi:10.7668/hbnxb.20193551

摘要/Abstract

摘要：

旨在初探BMF基因在小尾寒羊卵泡发育过程中的生物学功能,为将来深入探索其调控机理提供理论依据。对小尾寒羊实施同期发情,采用实时荧光定量PCR(Quantitative real-time PCR,RT-qPCR)、蛋白免疫印迹(Western Blot,WB)及免疫荧光染色(IF)方法检测BMF基因及其编码蛋白在不同生理阶段卵巢中的表达量;随后分离不同直径的卵泡,分析目的基因在大、中、小卵泡中的表达差异。结果显示,BMF mRNA在撤栓后42 h卵巢中的表达量显著高于撤栓后18 h(P<0.05),其编码蛋白表达结果与mRNA基本一致;BMF蛋白主要表达于卵泡颗粒细胞和卵泡膜细胞中;进一步分离卵泡,发现BMF基因及其编码蛋白表达量均在中卵泡中显著高于大卵泡和小卵泡(P<0.05)。以上结果表明,BMF基因可能参与卵巢周期性活动,在卵泡发育过程中发挥重要作用。

关键词: 小尾寒羊, BMF, 卵泡, 卵巢, 繁殖

Abstract:

The aim of this experiment was to investigate the biological function of BMF gene(Bcl-2 modifying factor)in the follicular development of Small-tailed Han Sheep,and to provide the theoretical basis for further exploration of its regulatory mechanism in the future.Firstly,the synchronization estrus was implemented in Small-tailed Han Sheep.Then,we used Quantitative Real-time PCR(RT-qPCR),Western Blot(WB)and Immunofluorescence staining(IF)to detect the expression of the BMF gene and its encoded protein in the ovaries at different physiological stages;subsequently,we isolated follicles of different diameters and analyzed the differences in expression of the target gene in large,middle and small follicles.The results showed that the expression of BMF mRNA was significantly higher in the ovary at 42 h after thrombectomy than at 18 h after thrombectomy(P<0.05),and the results of its encoded protein expression of BMF were basically consistent with the mRNA.BMF protein was mainly expressed in follicular granulosa cells and follicular membrane cells.We further isolated follicles and found that the expression of both the BMF gene and its encoded protein in middle follicles was significantly higher than that in large and small follicles(P<0.05).These results suggest that BMF gene may be involved in the ovarian cyclic activity and play an important role in follicular development.

Key words: Small-tailed Han Sheep, BMF, Follicle, Ovary, Reproduction

李营营, 张昊堃, 贾皓帆, 郭婧瑶, 杨喜喜, 王志亮, 李明娜, 王欣荣. 小尾寒羊BMF基因在发情不同阶段卵巢及卵泡发育过程中的表达分析[J]. 华北农学报, 2023, 38(2): 217-223. doi: 10.7668/hbnxb.20193551.

LI Yingying, ZHANG Haokun, JIA Haofan, GUO Jingyao, YANG Xixi, WANG Zhiliang, LI Mingna, WANG Xinrong. Expression Analysis of BMF Gene During the Ovaries at Different Stages of Estrus and Follicular Development in Small-tailed Han Sheep[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 217-223. doi: 10.7668/hbnxb.20193551.

http://www.hbnxb.net/CN/Y2023/V38/I2/217

图/表 6

表1 引物序列信息

Tab.1 Information of primer sequences

基因 Gene	引物序列(5'-3') Primer sequences (5'-3')	产物大小/bp Amplification size
BMF	F:CCAGTTCCATCGGCTTCATA	115
	R:CCCATTCCTGTTCTCATCTCC
β-actin	F:CTTCCAGCCTTCCTTCCTGG	180
	R:GCCAGGGCAGTGATCTCTTT

图1 BMF mRNA在小尾寒羊不同生理阶段卵巢中的表达不同字母表示差异显著(P<0.05)。图2,4,5同。

Fig.1 Expression of BMF mRNA in ovary of Small-tailed Han Sheep at different physiological stages The different letters means significant difference(P < 0.05). The same as Fig.2,4,5.

图2 BMF蛋白在小尾寒羊不同生理阶段卵巢中的表达 A.不同生理阶段卵巢中BMF蛋白印迹条带; B.BMF蛋白在不同生理阶段卵巢中的表达。

Fig.2 Expression of BMF protein in ovary of Small-tailed Han Sheep at different physiological stages A.BMF blotting bands in ovaries at different physiological stages;B.BMF protein expression in different physiological stages of ovary.

图3 BMF蛋白在小尾寒羊不同生理阶段卵巢中的分布情况(200×) GC.颗粒细胞;LC.间质细胞;TC.膜细胞。

Fig.3 Distribution of BMF protein in ovary of Small-tailed Han Sheep at different physiological stages(200×) GC.Granulosa cells;LC.Leydig cells;TC.Theca cells.

图4 BMF mRNA在不同直径卵泡中的表达

Fig.4 Expression of BMF mRNA in follicles of different diameters

图5 BMF蛋白在不同直径卵泡中的表达 A.不同直径卵泡中BMF蛋白印迹条带:S.小卵泡,M.中卵泡,L.大卵泡;B.BMF蛋白在不同直径卵泡中的表达。

Fig.5 Expression of BMF protein in follicles of different diameters A.BMF imprinted bands in follicles of different diameters:S.Small follicles,M.Middle follicles,L.Large follicles;B.Expression of BMF protein in follicles of different diameters.

参考文献 37

[1]	史金平. 基于比较转录组挖掘小尾寒羊及杜寒F1生长发育和肉质相关的差异表达基因[D]. 兰州: 甘肃农业大学, 2021.
	Shi J P. Based on the comparative transcriptome to discover the degs related to the growth,development and meat quality of Small-tail Han Sheep and Du han F1[D]. Lanzhou: Gansu Agricultural University, 2021.
[2]	国家畜禽遗传资源委员会组. 中国畜禽遗传资源志-羊志[M]. 北京: 中国农业出版社, 2011.
	China National Commission of Animal Genetic Resources. Animal genetic resources in China-sheep and goats[M]. Beijing: China Agriculture Press, 2011.
[3]	朱士恩. 家畜繁殖学[M]. 6版. 北京: 中国农业出版社, 2015.
	Zhu S E. Animal reproduction[M].6rd ed.ed. Beijing: China Agriculture Press, 2015.
[4]	郭晓飞. FecB基因影响小尾寒羊繁殖力的分子机制研究[D]. 北京: 中国农业大学, 2018.
	Guo X F. Study on molecular mechanism of FecB gene for fecundity in Small-tail Han Sheep[D]. Beijing: China Agricultural University, 2018.
[5]	李芝丰. FecBBB型和FecB⁺⁺型小尾寒羊卵泡期和黄体期输卵管转录组表达分析[D]. 扬州: 扬州大学, 2021. doi:10.27441/d.cnki.gyzdu.2021.000976. doi: 10.27441/d.cnki.gyzdu.2021.000976
	Li Z F. Transcriptome analysis of follicular and luteal oviducts of Small-tail Han Ship with FecB^BB type and FecB⁺⁺ genotypes[D]. Yangzhou: Yangzhou University, 2021.
[6]	Miao X Y, Luo Q M, Xie L L, Zhao H J, Qin X Y. Comparative DNA methylome analysis of estrus ewes reveals the complex regulatory pathways of sheep fecundity[J]. Reproductive Biology and Endocrinology, 2020, 18(1):77.doi:10.1186/s12958-020-00633-9. doi: 10.1186/s12958-020-00633-9 pmid: 32753034
[7]	张坤. 宁夏4个绵羊群体繁殖相关基因SNP的检测与分析[D]. 银川: 宁夏大学, 2018.
	Zhang K. Detection and analysis of SNP of reproductive related genes in four sheep populations in Ningxia[D]. Yinchuan: Ningxia University, 2018.
[8]	孙燕勇. 整合eGWAS和eQTL分析绵羊全血转录组与繁殖激素的关联[D]. 呼和浩特: 内蒙古农业大学, 2021.doi:10.27229/d.cnki.gnmnu.2021.000029. doi: 10.27229/d.cnki.gnmnu.2021.000029.
	Sun Y Y. Integrating eGWAS and eQTL to analyze the relationship between transcriptome of sheep whole blood and reproductive hormones[D]. Hohhot: Inner Mongolia Agricultural University, 2021.
[9]	曹晓涵. Bcl-2相关的凋亡基因在绵羊繁殖器官中的表达特征及Bad基因在绵羊发情周期中的作用[D]. 北京: 中国农业科学院, 2018.
	Cao X H. Expression characteristics of Bcl-2-related apoptosis gene in reproductive organs of sheep and the role of Bad gene in estrus cycle of sheep[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
[10]	李玉珍. BMF促细胞凋亡研究进展[J]. 生物化学与生物物理进展, 2017, 44(9):751-756.doi:10.16476/j.pibb.2016.0318. doi: 10.16476/j.pibb.2016.0318
	Li Y Z. Research progress on BMF proapoptosis[J]. Progress in Biochemistry and Biophysics, 2017, 44(9):751-756.
[11]	Hunt A, Evan G. Till death us do part[J]. Science, 2001, 293(5536):1784-1785.doi:10.1126/science.11546862. doi: 10.1126/science.11546862 pmid: 11546862
[12]	Puthalakath H, Villunger A, O'Reilly L A, Beaumont J G, Coultas L, Cheney R E, Huang D C, Strasser A. Bmf:A proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex,activated by anoikis[J]. Science, 2001, 293(5536):1829-1832.doi:10.1126/science.1062257. doi: 10.1126/science.1062257 pmid: 11546872
[13]	Albamonte M I, Albamonte M S, Bou-Khair R M, Zuccardi L, Daniel Vitullo A. The ovarian germinal reserve and apoptosis-related proteins in the infant and adolescent human ovary[J]. Journal of Ovarian Research, 2019, 12(1):22.doi:10.1186/s13048-019-0496-2. doi: 10.1186/s13048-019-0496-2 pmid: 30857552
[14]	Vaskivuo T E, Tapanainen J S. Apoptosis in the human ovary[J]. Reproductive Biomedicine Online, 2003, 6(1):24-35.doi:10.1016/s1472-6483(10)62052-4. doi: 10.1016/s1472-6483(10)62052-4 pmid: 12626140
[15]	Hartley P S, Bayne R A L, Robinson L L L, Fulton N, Anderson R A. Developmental changes in expression of myeloid cell leukemia-1 in human germ cells during oogenesis and early folliculogenesis[J]. The Journal of Clinical Endocrinology & Metabolism, 2002, 87(7):3417-3427.doi:10.1210/jcem.87.7.8644. doi: 10.1210/jcem.87.7.8644 URL
[16]	Aitken R J, Findlay J K, Hutt K J, Kerr J B. Apoptosis in the germ line[J]. Reproduction, 2011, 141(2):139-150.doi:10.1530/REP-10-0232. doi: 10.1530/REP-10-0232 pmid: 21148286
[17]	Vaithiyanathan K, Liew S H, Zerafa N, Gamage T, Cook M, O'Reilly L A, Bouillet P, Scott C L, Strasser A, Findlay J K, Hutt K J. BCL2-modifying factor promotes germ cell loss during murine oogenesis[J]. Reproduction (Cambridge, England), 2016, 151(5):553-562.doi:10.1530/rep-15-0561. doi: 10.1530/REP-15-0561 pmid: 26917450
[18]	Liew S H, Vaithiyanathan K, Cook M, Bouillet P, Scott C L, Kerr J B, Strasser A, Findlay J K, Hutt K J. Loss of the proapoptotic BH3-only protein BCL-2 modifying factor prolongs the fertile life span in female mice[J]. Biology of Reproduction, 2014, 90(4):1-9,77.doi:10.1095/biolreprod.113.116947. doi: 10.1095/biolreprod.113.116947
[19]	Liew S H, Nguyen Q N, Strasser A, Findlay J K, Hutt K J. The ovarian reserve is depleted during puberty in a hormonally driven process dependent on the pro-apoptotic protein BMF[J]. Cell Death & Disease, 2017, 8(8):e2971.doi:10.1038/cddis.2017.361. doi: 10.1038/cddis.2017.361
[20]	Li M N, Liang W W, Zhu C Y, Qin S Z. Smad4 mediates Bmf involvement in sheep granulosa cell apoptosis[J]. Gene, 2022, 817:146231.doi:10.1016/j.gene.2022.146231. doi: 10.1016/j.gene.2022.146231 URL
[21]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCTmethod[J]. Methods, 2001, 25(4):402-408.doi:10.1006/meth.2001.1262. doi: 10.1006/meth.2001.1262 pmid: 11846609
[22]	Miranda V O, Oliveira F C, Dias J H, Vargas Júnior S F V, Goularte K L, Sá Filho M F, Sá Filho O G S, Baldassarre H, Vieira A D, Lucia T, Gasperin B G. Estrus resynchronization in ewes with unknown pregnancy status[J]. Theriogenology, 2018, 106:103-107.doi:10.1016/j.theriogenology.2017.10.019. doi: S0093-691X(17)30493-4 pmid: 29049921
[23]	Rubianes E, Menchaca A, Carbajal B. Response of the 1-5 day-aged ovine corpus luteum to prostaglandin F2alpha[J]. Animal Reproduction Science, 2003, 78(1/2):47-55.doi:10.1016/s0378-4320(03)00046-0. doi: 10.1016/s0378-4320(03)00046-0 URL
[24]	陈维稷. 通过调整卵泡募集改善绵羊同期发情效果的研究[D]. 泰安: 山东农业大学, 2021.doi:10.27277/d.cnki.gsdnu.2021.000959. doi: 10.27277/d.cnki.gsdnu.2021.000959.
	Chen W J Study on improving the effect of estrus synchronization by modulating follicular recruitment in sheep[D]. Taian: Shandong Agricultural University, 2021.
[25]	张英杰, 刘月琴, 储明星. 小尾寒羊高繁殖力和常年发情内分泌机理的研究[J]. 畜牧兽医学报, 2001, 32(6):510-516.doi:10.3321/j.issn:0366-6964.2001.06.006. doi: 10.3321/j.issn:0366-6964.2001.06.006
	Zhang Y J, Liu Y Q, Chu M X. Study on endocrine basis of prolificacy and non-seasonal estrus in Small-tail Han Sheep[J]. Acta Vetereinaria et Zootechnica Sinica, 2001, 32(6):510-516.
[26]	汤继顺. 利用转录组测序和蛋白质组学分析筛选绵羊多羔候选基因的研究[D]. 北京: 中国农业科学院, 2019.
	Tang J S. Screening candidate genes for sheep multiparous by transcriptome sequencing and protein omics analysis[D]. Beijing: Chinese Academy of Agricultural Sciences, 2019.
[27]	Hashemi M, Safdarian M, Kafi M. Estrous response to synchronization of estrus using different progesterone treatments outside the natural breeding season in ewes[J]. Small Ruminant Research, 2006, 65(3):279-283.doi:10.1016/j.smallrumres.2005.07.051. doi: 10.1016/j.smallrumres.2005.07.051 URL
[28]	孙爽. 奶山羊重复同期发情处理对生殖激素及卵巢组织基因转录表达的影响[D]. 杨凌: 西北农林科技大学, 2019. doi:10.27409/d.cnki.gxbnu.2019.000228. doi: 10.27409/d.cnki.gxbnu.2019.000228
	Sun S. Effects of repeated estrus synchronization treatment on reproductive hormones and gene transcription and expression in ovarian tissue of dairy goats[D]. Yangling: Northwest A&F University, 2019.
[29]	郭亚军, 柳苗苗, 付德海, 冉兴荣, 王欣荣. 藏绵羊卵巢组织学及卵泡超微形态的观察[J]. 畜牧兽医学报, 2021, 52(2):389-398.doi:10.11843/j.issn.0366-6964.2021.02.011. doi: 10.11843/j.issn.0366-6964.2021.02.011
	Guo Y J, Liu M M, Fu D H, Ran X R, Wang X R. Observation of ovary histology and ultrastructure of follicles in Tibetan sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2):389-398.
[30]	钱云, 丁家桐, 刘红林. 卵泡生长发育的动态模式及其调控[J]. 中国畜牧杂志, 2000, 36(1):38-40.doi:10.3969/j.issn.0258-7033.2000.01.017. doi: 10.3969/j.issn.0258-7033.2000.01.017
	Qian Y, Ding J T, Liu H L. Dynamic pattern and regulation of follicular growth and development[J]. Chinese Journal of Animal Science, 2000, 36(1):38-40.
[31]	Jing J J, Jiang X L, Chen J W, Yao X L, Zhao M M, Li P F, Pan Y Y, Ren Y S, Liu W Z, Lyu L H. Notch signaling pathway promotes the development of ovine ovarian follicular granulosa cells[J]. Animal Reproduction Science, 2017, 181:69-78.doi:10.1016/j.anireprosci.2017.03.017. doi: S0378-4320(16)30722-9 pmid: 28400072
[32]	Pan Y, Zhu J Z, L Q, Shi D S, Yang S F, Xing Q H, Zhang R M, Cheng J R, Deng Y F. Follicle-stimulating hormone regulates glycolysis of water buffalo follicular granulosa cells through AMPK/SIRT1 signalling pathway[J]. Reproduction in Domestic Animals, 2022, 57(2):185-195.doi:10.1111/rda.14039. doi: 10.1111/rda.14039 URL
[33]	李暄, 佟俊硕, 张大崇, 王丙云, 陈胜锋, 刘璨颖. miRNA调节卵泡颗粒细胞凋亡及其机制的研究进展[J]. 中国畜牧兽医, 2021, 48(12):4429-4441.doi:10.16431/j.cnki.1671-7236.2021.12.011. doi: 10.16431/j.cnki.1671-7236.2021.12.011
	Li X, Tong J S, Zhang D C, Wang B Y, Chen S F, Liu C Y. Research advances on miRNA in regulating the apoptosis of follicular granulosa cells and its mechanism[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(12):4429-4441.
[34]	王楠, 张瑞, 潘阳阳, 何翃宏, 王靖雷, 崔燕, 余四九. 牦牛TGF-β1基因克隆及在雌性生殖系统主要器官中的表达定位[J]. 生物技术通报, 2022, 38(6):279-290.doi:10.13560/j.cnki.biotech.bull.1985.2021-1152. doi: 10.13560/j.cnki.biotech.bull.1985.2021-1152
	Wang N, Zhang R, Pan Y Y, He H H, Wang J L, Cui Y, Yu S J. Cloning of Bos grunniens TGF-β1 gene and its expression in major organs of female reproductive system[J]. Biotechnology Bulletin, 2022, 38(6):279-290.
[35]	郭亚军. 藏绵羊卵巢的组织形态特征及相关因子在不同时期的表达与分布[D]. 兰州: 甘肃农业大学, 2021.doi:10.27025/d.cnki.ggsnu.2021.000066. doi: 10.27025/d.cnki.ggsnu.2021.000066
	Guo Y J.. Histomorphological characteristics of Tibetan sheep ovary and the expression and distribution of related factors in different periods[D]. Lanzhou: Gansu Agricultural University, 2021.
[36]	薛丽娜, 毕锡麟, 王锴, 党文庆, 韩琦, 罗惠娣, 曹宁贤, 吕丽华. WNT2在绵羊卵泡颗粒细胞的表达及功能研究[J]. 畜牧兽医学报, 2020, 51(1):74-82.doi:10.11843/j.issn.0366-6964.2020.01.009. doi: 10.11843/j.issn.0366-6964.2020.01.009
	Xue L N, Bi X L, Wang K, Dang W Q, Han Q, Luo H D, Cao N X, Lü L H. Expression and function analysis of WNT2 in ovine follicular granulosa cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1):74-82.
[37]	宋鹏琰, 锡建中, 张振红, 付强, 岳巧娴, 张配颖, 周荣艳. 绵羊miR-200b对卵泡颗粒细胞周期和凋亡的影响[J]. 畜牧兽医学报, 2021, 52(12):3471-3479.doi:10.11843/j.issn.0366-6964.2021.012.014. doi: 10.11843/j.issn.0366-6964.2021.012.014
	Song P Y, Xi J Z, Zhang Z H, Fu Q, Yue Q X, Zhang P Y, Zhou R Y. Effects of ovine miR-200b on cell cycle and apoptosis of follicular granulosa cells[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12):3471-3479.

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小尾寒羊BMF基因在发情不同阶段卵巢及卵泡发育过程中的表达分析

Expression Analysis of BMF Gene During the Ovaries at Different Stages of Estrus and Follicular Development in Small-tailed Han Sheep

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