摘要： 为研究牦牛肿瘤抑制蛋白Tp53(Tumor protein p53)的基因序列特征及其在牦牛卵巢中的表达情况,采集不同发情时期牦牛卵巢.根据黄牛的基因序列设计5'到3'端特异性引物,RT-PCR扩增基因克隆得到Tp53 基因,并对其基因结构等其他生物信息进行分析.采用Real-time PCR方法分析牦牛不同发情时期卵巢中Tp53 基因的表达差异.结果显示:牦牛Tp53 基因序列的编码区为1 161 bp,编码386个氨基酸.相似性与进化分析显示,与瘤牛Tp53 基因的相似性最高,达到98.4%,与家猫的相似性最低,为80.7%,表明Tp53 基因在进化中具有高度保守性;Real-time PCR检测结果显示,Tp53 基因在各个发情时期卵巢中均有表达,且表达量差异显著(P<0.05).Tp53 基因的表达量出现差异可能是由于在不同发情时期卵巢细胞DNA损伤程度及内分泌等因素的不同所致.牦牛Tp53 基因的成功克隆及生物信息学分析为该基因的进一步研究奠定了一定基础.

关键词: 牦牛, Tp53, 基因克隆, 生物信息学, 卵巢

Abstract: In order to research characteristics of yak Tp53 gene and its expression in different estrus period ovary.Gathered the ovarian during in different periods.The specific primers were on the basis of conservative Tp53 gene sequence of cattle,the gene sequence could be obtained by cloning,analyzed its gene structure and bioinformatics.Real-time PCR method for Tp53 gene expression in yak different estrus period ovary.The results showed Tp53 gene sequence coding region of 1 161 bp,and encoding 386 amino acids on yak.Homology and evolutionary analysis showed the highest homology with Tp53 gene,it could reach 98.4%,the lowest with domestic cats,80.7%,indicated that Tp53 gene was highly conserved in evolution;Real-time PCR detection results showed that the expression of Tp53 in each period was significant different( P<0.05).Tip:the reason why the expression of Tp53 was different might be due to the extent of DNA damage and endocrine factors in different estrus period ovaries.The successful cloning and bioinformatics analysis in yak Tp53 laid a foundation for the further study.

Key words: Bos grunniens, Tp53, Cloning, Bioinformatics, Ovary

中图分类号: 

• S823

• Q78