虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆

doi:10.7668/hbnxb.2010.01.012

华北农学报 ›› 2010, Vol. 25 ›› Issue (1): 61-67. doi: 10.7668/hbnxb.2010.01.012

所属专题： 生物技术；

虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆

王辉^1,2, 张秀清¹, 胡玉红³, 孙君社¹

1. 中国农业大学,食品科学与营养工程学院, 北京 100083;
2. 中国农业大学,食品科学与营养工程学院, 北京 100083;
3. 河北师范大学,实验中心, 河北石家庄 050016

收稿日期:2009-11-02 出版日期:2010-02-28
通讯作者: 孙君社(1961-),男,河北栾城人,教授,博士,主要从事生物技术在农产品加工中应用和酶与发酵方面的教学与研究.
作者简介:王辉(1981-),男,河北石家庄人,博士,主要从事蛙类功能性多肽研究.

Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs

WANG Hui1^1,2, 2¹, ZHANG Xiu-qing1³, HU Yu-hong3¹

1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2. College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China;
3. Experimental Center, Hebei Normal University, Shijiazhuang 050016, China

Received:2009-11-02 Published:2010-02-28

摘要/Abstract

摘要： 运用SMART技术成功构建了虎纹蛙(Hoplobatrachus rugulosus)皮肤组织全长cDNA文库.根据蛙科动物抗菌肽的信号肽保守序列设计简并引物,以PCR法成功从文库中随机挑选的阳性克隆中筛选出2个抗菌肽cDNA序列,命名为tigerinin-HR1和tigerinin-HR2.这2个抗菌肽cDNA序列是首次从虎纹蛙皮肤组织中克隆出的抗菌肽编码基因.

关键词: 虎纹蛙, cDNA文库, 抗菌肽, tigerinin

Abstract: A full length cDNA library of Hoplobatrachus rugulosus skin tissue was constructed with the SMART technique.A special primer was designed from a highly conserved domain of the signal peptide of antimicrobial peptide encoding cDNAs from ranid frogs.Two different cDNA sequences encoding precursor proteins named tigerinin-HR1 and tigerinin-HR2 were screened from the library by PCR.tigerinin-HR1 and tigerinin-HR2 were the first found encoding cDNA sequences of antimicrobial peptide from H.rugulosus.

Key words: Hoplobatrachus rugulosus, cDNA library, Antimicrobial peptides, tigerinin

中图分类号:

Q785

王辉, 张秀清, 胡玉红, 孙君社. 虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆[J]. 华北农学报, 2010, 25(1): 61-67. doi: 10.7668/hbnxb.2010.01.012.

WANG Hui1,2,ZHANG Xiu-qing1,HU Yu-hong3,SUN Jun-she1. Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 61-67. doi: 10.7668/hbnxb.2010.01.012.

http://www.hbnxb.net/CN/Y2010/V25/I1/61

参考文献

[1] 罗琛,刘楚吾,姚占州.虎纹蛙人工养殖的研究初报[J].湖南师范大学:自然科学学报,1992,15(2):175-178.
[2] Ganz T.Defensins:antimicrobial peptides of innate immunity[J].Nat Rev Immunol,2003,3(9):710-720.
[3] Skerlavaj B,Benincasa M,Risso A,et al.SMAP-29:A potent antibacterial and antifungal peptide from sheep leucocytes[J].FEBS Lett,1999,463(1-2):58-62.
[4] Wang H X,Ng T B.Isolation and characterization of an antifungal peptide with antiproliferative activity from seeds of Phaseolus vulgaris cv.'Spotted Bean'[J].Appl Microbiol Biotechnol,2007,74(1):125-130.
[5] Wu S,Jia S,Sun D,et al.Purification and characterization of two novel antimicrobial peptides Subpeptin JM4-A and subpeptin JM4-B produced by Bacillus subtilis JM4[J].Curr Microbiol,2005,51(5):292-296.
[6] Simmaco M,Mignogna G,Canofeni S,et al.Temporins,antimicrobial peptides from the European red frog Rana temporaria[J].Eur J Biochem,1996,242(3):788-792.
[7] Morikawa N,Hagiwara K,Nakajima T.Brevinin-1 and-2,unique antimicrobial peptides from the skin of the frog,Rana brevipoda porsa[J].Biochem Biophys Res Commun,1992,189(1):184-190.
[8] Simmaco M,Mignogna G,Barra D,et al.Antimicrobial peptides from skin secretions of Rana esculenta.Molecular cloning of cDNAs encoding esculentin and brevinins and isolation of new active peptides[J].J Biol Chem,1994,269(16):11956-11961.
[9] Isaacson T,Soto A,Iwamuro S,et al.Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica[J].Peptides,2002,23(3):419-425.
[10] Rao X C,Li S,Hu J C,et al.A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli[J].Protein Expr Purif,2004,36(1):11-18.
[11] Xu Z,Peng L,Zhong Z,et al.High level expression of a soluble functional antimicrobial peptide,human beta-defensin 2,in Escherichia coli[J].Biotechnol Prog,2006,22(2):382-386.
[12] Sai K P,Jagannadham M V,Vairamani M,et al.Tigerinins:novel antimicrobial peptides from the Indian frog Rana tigerina[J].J Biol Chem,2001,276(4):2701-2707.
[13] Song Y,Lu Y,Wang L,et al.Purification,characterization and cloning of two novel tigerinin-like peptides from skin secretions of Fejervarya cancrivora[J].Peptides,2009,30(7):1228-1232.
[14] Chen T,Li L,Zhou M,et al.Amphibian skin peptides and their corresponding cDNAs from single lyophilized secretion samples:identification of novel brevinins from three species of Chinese frogs[J].Peptides,2006,27(1):42-48.
[15] Suzuki H,Iwamuro S,Ohnuma A,et al.Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii[J].Peptides,2007,28(3):505-514.
[16] Mangoni M L.Temporins,anti-infective peptides with expanding properties[J].Cell Mol Life Sci,2006,63(2006):1060-1069.
[17] Park Y,Lee D G,Jang S H,et al.A Leu-Lys-rich antimicrobial peptide:activity and mechanism[J].Biochim Biophys Acta,2003,1645(2):172-182.
[18] Huang L,Ching C B,Jiang R,et al.Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression[J].Protein Expr Purif,2008,61(2):168-174.
[19] Zhou M,Chen T,Walker B,et al.Pelophylaxins:novel antimicrobial peptide homologs from the skin secretion of the Fukien gold-striped pond frog,Pelophylax plancyi fukienensis:identification by "shotgun" cDNA cloning and sequence analysis[J].Peptides,2006,27(1):36-41.
[20] Chen T,Zhou M,Rao P,et al.The Chinese bamboo leaf odorous frog(Rana(Odorrana)versabilis)and North American Rana frogs share the same families of skin antimicrobial peptides[J].Peptides,2006,27(7):1738-1744.
[21] Zhou M,Chen T,Walker B,et al.Lividins:novel antimicrobial peptide homologs from the skin secretion of the Chinese Large Odorous frog,Rana(Odorrana) livida.Identification by "shotgun" cDNA cloning and sequence analysis[J].Peptides,2006,27(9):2118-2123.
[22] 王辉,张秀清,胡玉红,等.阔褶水蛙皮肤组织cDNA文库的构建及抗菌肽brevinin 2-LTs的cDNA克隆和序列分析[J].华北农学报,2009,24(增刊):9-14.
[23] Frost D R,Grant T,Faivovich J,et al.The amphibian tree of life[J].Bull Am Mus Nat His,2006,297:1-370.
[24] Conlon J M,Kolodziejek J,Nowotny N,et al.Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog,Rana dybowskii[J].Toxicon,2007,50(6):746-756.
[25] Conlon J M,Kolodziejek J,Nowotny N.Antimicrobial peptides from ranid frogs:taxonomic and phylogenetic markers and a potential source of new therapeutic agents[J].Biochimica Biophysica Acta,2004,1696(1):1-14.

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虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆

Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs

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虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆

Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs

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