苜蓿叶片PSⅡ和光能分配的日变化特性

doi:10.7668/hbnxb.2009.S2.029

华北农学报

苜蓿叶片PSⅡ和光能分配的日变化特性

杨丹霞^1,2, 孙广玉¹, 蔡敦江², 胡彦波¹

1 东北林业大学生命科学学院黑龙江哈尔滨 150040;
2 黑龙江省农垦科学院农作物研究所黑龙江佳木斯 154007

收稿日期:2009-10-14 出版日期:2009-12-31
通讯作者: 孙广玉(1963-), 男, 黑龙江巴彦人, 教授, 主要从事植物生理学研究.
作者简介:杨丹霞(1964-), 女, 山西保德人, 副研究员, 硕士, 主要从事植物育种和栽培生理研究.
基金资助:
黑龙江省重大科技攻关项目(GA06A301);黑龙江省重点科技攻关项目(GB07B306)

The Diurnal Changes of PS Ⅱ and Absorbed Light Allocation in Leaves of Alfalfa(Medicago sativa L.)

YANG Dan-xia^1,2, SUN Guang-yu¹, CAI Dun-jiang², HU Yan-bo¹

1 College of Life Science, Northeast Forest University, Harbin 150040, China;
2 Institute of Crops, Heilongjiang Academy of Land Reclamation and Agricultural Science, Jiamusi 154007, China

Received:2009-10-14 Published:2009-12-31

摘要/Abstract

摘要： 以自育苜蓿品种越冬之星为试验材料, 利用叶绿素荧光技术, 研究了苜蓿叶片PSⅡ和PSⅡ最大光能转化效率(Fv/Fm)的日变化及其光能分配特性。结果表明, 苜蓿叶片Fv/Fm和实际光化学效率(ΦPSⅡ)的日变化均呈现"双峰"曲线, 中午期间的PSⅡ功能下调, 有明显的光抑制。在光合有效辐射(PAR)逐渐增强过程中, Fv/Fm和ΦPSⅡ降低, 非光化学猝灭(NPQ)增加, 激发能由PSⅠ向PSⅡ分配比例增加, 说明引起苜蓿光抑制的原因除了NPQ之外, 分配给PSⅡ能量增加后进一步促进PSⅡ可逆失活是非常重要的因素。PAR逐渐减小时, 光抑制逐渐减缓, 光化学效率恢复, 这主要是由于NPQ减小, 激发能分配不平衡性系数(β/α-1)下降, PSⅡ能量负荷减轻, 失活的PSⅡ恢复活性的结果。

关键词: 苜蓿, 叶绿素荧光, 非光化学猝灭, 状态转换

Abstract: A study was conducted, using chlorophyll fluorescence technique and a local alfalfa variety Yuedongzhixing as experimental material, to investigate the diurnal changes of PS Ⅱ and maximum quantum efficiency of photosystem Ⅱ photochemistry(Fv/Fm) in alfalfa leaves. The data acquired in this study showed that the diurnal changes of Fv/Fm and actual photochemical efficiency of PS Ⅱ (φPS Ⅱ) were related to light intensity, the functional photosystem Ⅱ reaction centers were decreased to cause significant photoinhibition. With the increasing of photosynthetic actual irradiation (PAR)to simulate PAR diurnal change, and φPS Ⅱ decreased, nonphotochemical quenching (NPQ)increased, and the fraction of excited energy allocated to PS Ⅱ increased to enhance PS Ⅱ burden carrying excited energy. These results indicated that photoinhibition in alfalfa leaves were caused primarily by increasing of excited energy allocated to PS Ⅱ to enhance reversible inactivation of PS Ⅱ centers except NPQ increasing. With the decreasing of PAR, Fv/Fm and φPS Ⅱ increased, NPQ and unbalance index of excited energy allocated to PS Ⅱ and PS Ⅰ (β/α -1) declined respectively. This indicated that the recovery of PS Ⅱ photochemical efficiency was caused primarily by renewing PS Ⅱ activation owing to unloading the energy burden of PS Ⅱ.

Key words: Medicago sativa L., Chlorophyll fluorescence, Nonphotochemical quenching, State transition

中图分类号:

S541.9

YANG Dan-xia, SUN Guang-yu, CAI Dun-jiang, HU Yan-bo. The Diurnal Changes of PS Ⅱ and Absorbed Light Allocation in Leaves of Alfalfa(Medicago sativa L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.029.

http://www.hbnxb.net/CN/Y2009/V24/IS2/130

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苜蓿叶片PSⅡ和光能分配的日变化特性

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