摘要： 研究了牛体细胞核移植过程的去核方法、融合条件等对重构胚发育的影响，旨在探索一种简化的核移植操作程序。结果表明，添加FSH，LH，最高成熟率为81.7%。在电压为2.6 kV/cm时，融合率为93.96%。激活后重构胚在压制的WOW内进行培养，采用半卵法去核的卵裂率和8-细胞发育率分别为61.2%和3.27%，盲切法去核的卵裂率和8-细胞发育率分别是 70.8%和7.6%。

关键词: 体细胞, 核移植, 徒手克隆, 牛

Abstract: The research was designed to explore a brief procedure on nuclear transfer,which included compare of di-f ferent methods for mature culture of bovine occytes,separation and general culture of fibroblast taken from the skin of bovine,enucleated method and condit ion on fusion which exactly affect the development of reconstructed embryos.Finally the highest mature rate was 81.7% with FSH and LH added,and fusion rate was 93.96% with voltage of 2.6 kV/cm.The cleavage rate and 8-cell development rate of embryos the percentage was 61.2% and 3.27% respect ively when two-half cytoplas-t method was applied,and the data up to 70.8% and 7.56% while blind-cutting manipulation was adopted,and these outcome were all obtained under the condition that reconstructed embryos were cultured in pressing WOW after activation.

Key words: Somatic cell, Nuclear transfer, Handmade cloning, Bovine

中图分类号: 

• S823